(Circular) (Six-base) MAPSORT of: pFN21AE2390.seq Check: 7646 from: 1 to: 6208 pFN21AE2390 6208 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2089 3551 1937 Size: 152 1462 4594 Fragments arranged by size: 4594 1462 152 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3392 4271 5655 5923 1632 Size: 1760 879 1384 268 1917 Fragments arranged by size: 1917 1760 1384 879 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 5107 6084 1203 Size: 3904 977 1327 Fragments arranged by size: 3904 1327 977 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 4937 4937 Size: 6208 AleI CACnn'nnGTG Cuts at: 1166 2231 2504 1166 Size: 1065 273 4870 Fragments arranged by size: 4870 1065 273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6208 AlwNI CAG_nnn'CTG Cuts at: 2454 3951 5523 2454 Size: 1497 1572 3139 Fragments arranged by size: 3139 1572 1497 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 2368 3517 3743 3895 6004 1493 Size: 875 1149 226 152 2109 1697 Fragments arranged by size: 2109 1697 1149 875 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2434 3098 3533 1870 Size: 73 491 664 435 4545 Fragments arranged by size: 4545 664 491 435 73 AvrII C'CTAG_G Cuts at: 2675 2675 Size: 6208 BamHI G'GATC_C Cuts at: 3538 3905 3538 Size: 367 5841 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 3529 4984 618 Size: 325 217 627 260 1482 1455 1842 Fragments arranged by size: 1842 1482 1455 627 325 260 217 BanII G_rGCy'C Cuts at: 1391 2020 2841 3123 3527 1391 Size: 629 821 282 404 4072 Fragments arranged by size: 4072 821 629 404 282 BbeI G_GCGC'C Cuts at: 2051 2051 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2794 928 Size: 971 895 4342 Fragments arranged by size: 4342 971 895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4100 5310 4100 Size: 1210 4998 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BfrBI ATG'CAT Cuts at: 2772 2772 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2650 4819 136 Size: 107 122 71 631 605 978 2169 1525 Fragments arranged by size: 2169 1525 978 631 605 122 107 71 BglII A'GATC_T Cuts at: 3048 6203 3048 Size: 3155 3053 BlpI GC'TnA_GC Cuts at: 2267 3625 2267 Size: 1358 4850 Bme1580I G_kGCm'C Cuts at: 1790 4269 5425 1790 Size: 2479 1156 2573 Fragments arranged by size: 2573 2479 1156 BmgBI CAC'GTC Cuts at: 1318 2341 2377 1318 Size: 1023 36 5149 Fragments arranged by size: 5149 1023 36 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3939 4897 442 Size: 416 53 83 399 24 2522 958 1753 Fragments arranged by size: 2522 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3331 3331 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2649 3123 3477 4868 1239 Size: 1028 382 474 354 1391 2579 Fragments arranged by size: 2579 1391 1028 474 382 354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4333 5198 5496 4333 Size: 865 298 5045 Fragments arranged by size: 5045 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2145 2180 2897 3099 4871 882 Size: 617 646 35 717 202 1772 2219 Fragments arranged by size: 2219 1772 717 646 617 202 35 BsaAI yAC'GTr Cuts at: 493 1204 3477 3999 493 Size: 711 2273 522 2702 Fragments arranged by size: 2702 2273 711 522 BsaBI GATnn'nnATC Cuts at: 6202 6202 Size: 6208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 2064 3471 4015 Size: 53 83 186 936 515 16 1407 544 Cuts at: 4015 4397 5032 275 Size: 382 635 1451 Fragments arranged by size: 1451 1407 936 635 544 515 382 186 83 53 16 BsaWI w'CCGG_w Cuts at: 3180 3295 4641 5039 5313 5460 5871 3180 Size: 115 1346 398 274 147 411 3517 Fragments arranged by size: 3517 1346 411 398 274 147 115 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2142 2172 1178 Size: 30 165 30 739 30 5214 Fragments arranged by size: 5214 739 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3307 1186 Size: 2121 4087 BseYI C'CCAG_C Cuts at: 2032 2041 2271 3150 5411 5907 2032 Size: 9 230 879 2261 496 2333 Fragments arranged by size: 2333 2261 879 496 230 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2984 1163 Size: 573 1248 4387 Fragments arranged by size: 4387 1248 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3658 4419 5447 1540 Size: 275 186 1657 761 1028 2301 Fragments arranged by size: 2301 1657 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2228 2289 4269 4354 5425 2228 Size: 61 1980 85 1071 3011 Fragments arranged by size: 3011 1980 1071 85 61 BsmI GAATG_Cn' Cuts at: 3719 3812 3719 Size: 93 6115 BsmBI CGTCTCn'nnnn_ Cuts at: 3319 3319 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2228 2289 2841 3123 3527 4269 Size: 399 230 208 61 552 282 404 742 Cuts at: 4269 4354 5425 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 404 399 282 230 208 85 61 BspEI T'CCGG_A Cuts at: 3180 3295 5871 3180 Size: 115 2576 3517 Fragments arranged by size: 3517 2576 115 BspHI T'CATG_A Cuts at: 2596 4097 5827 2596 Size: 1501 1730 2977 Fragments arranged by size: 2977 1730 1501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2817 3006 3084 3563 844 Size: 1973 189 78 479 3489 Fragments arranged by size: 3489 1973 479 189 78 BsrBI CCG'CTC Cuts at: 1394 1823 2053 3009 4095 1394 Size: 429 230 956 1086 3507 Fragments arranged by size: 3507 1086 956 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 2760 4703 4877 65 Size: 1183 1512 1943 174 1396 Fragments arranged by size: 1943 1512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4852 5039 1700 Size: 110 3042 187 2869 Fragments arranged by size: 3042 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 2454 794 Size: 1660 4548 BstBI TT'CG_AA Cuts at: 5012 5012 Size: 6208 BstEII G'GTnAC_C Cuts at: 1184 2467 1184 Size: 1283 4925 BstXI CCAn_nnnn'nTGG Cuts at: 3403 3403 Size: 6208 BstYI r'GATC_y Cuts at: 1972 3048 3538 3905 4290 4307 5759 5845 5857 Size: 1076 490 367 385 17 1452 86 12 Cuts at: 5857 6203 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 490 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2068 2949 3396 513 Size: 552 1003 881 447 3325 Fragments arranged by size: 3325 1003 881 552 447 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3316 487 Size: 683 56 2090 3379 Fragments arranged by size: 3379 2090 683 56 BtsI GCAGTG_nn' Cuts at: 3724 4518 4538 3724 Size: 794 20 5394 Fragments arranged by size: 5394 794 20 ClaI AT'CG_AT Cuts at: 1554 2298 3264 1554 Size: 744 966 4498 Fragments arranged by size: 4498 966 744 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2393 5215 809 Size: 507 1077 2822 1802 Fragments arranged by size: 2822 1802 1077 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2132 2620 2790 Size: 54 1425 50 275 18 302 488 170 Cuts at: 2790 3393 3655 4544 6173 8 Size: 603 262 889 1629 43 Fragments arranged by size: 1629 1425 889 603 488 302 275 262 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3655 1537 Size: 275 1843 4090 Fragments arranged by size: 4090 1843 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 2747 2790 3209 3419 4138 1353 Size: 858 536 43 419 210 719 3423 Fragments arranged by size: 3423 858 719 536 419 210 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2096 2333 2717 4795 5169 5315 Size: 1064 138 507 237 384 2078 374 146 Cuts at: 5315 387 Size: 1280 Fragments arranged by size: 2078 1280 1064 507 384 374 237 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2649 3123 3392 3477 4271 4868 Size: 393 635 382 474 269 85 794 597 Cuts at: 4868 5655 5923 1239 Size: 787 268 1524 Fragments arranged by size: 1524 794 787 635 597 474 393 382 269 268 85 EcoNI CCTnn'n_nnAGG Cuts at: 2679 2679 Size: 6208 EcoO109I rG'GnC_Cy Cuts at: 2275 2561 3450 2275 Size: 286 889 5033 Fragments arranged by size: 5033 889 286 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2254 2758 2254 Size: 504 5704 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2042 2051 2701 5355 1678 Size: 364 9 650 2654 2531 Fragments arranged by size: 2654 2531 650 364 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2142 2174 4849 4881 4923 Size: 32 135 32 570 32 2675 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2675 2626 570 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2090 2755 3552 3798 669 Size: 988 281 152 665 797 246 3079 Fragments arranged by size: 3079 988 797 665 281 246 152 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2047 2047 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 6084 6084 Size: 6208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 5322 5506 1305 Size: 1128 2889 184 2007 Fragments arranged by size: 2889 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2622 2792 3395 10 Size: 54 1425 343 790 170 603 2823 Fragments arranged by size: 2823 1425 790 603 343 170 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2504 2595 3401 4166 4525 4684 Size: 648 1065 273 91 806 765 359 159 Cuts at: 4684 518 Size: 2042 Fragments arranged by size: 2042 1065 806 765 648 359 273 159 91 MspA1I CmG'CkG Cuts at: 1606 2070 2454 3070 3343 3624 4301 5449 5694 Size: 464 384 616 273 281 677 1148 245 Cuts at: 5694 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 616 464 384 281 273 245 NarI GG'CG_CC Cuts at: 2048 2048 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 2949 3396 513 Size: 552 1884 447 3325 Fragments arranged by size: 3325 1884 552 447 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NsiI A_TGCA'T Cuts at: 2774 2774 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2012 1348 Size: 664 5544 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 3146 1108 Size: 2038 4170 PfoI T'CCnGG_A Cuts at: 2094 3139 3465 2094 Size: 1045 326 4837 Fragments arranged by size: 4837 1045 326 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2561 3450 2275 Size: 286 889 5033 Fragments arranged by size: 5033 889 286 PshAI GACnn'nnGTC Cuts at: 1613 3455 1613 Size: 1842 4366 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PstI C_TGCA'G Cuts at: 830 2459 2550 3348 3560 830 Size: 1629 91 798 212 3478 Fragments arranged by size: 3478 1629 798 212 91 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2454 3343 2454 Size: 889 5319 SacII CC_GC'GG Cuts at: 2071 2071 Size: 6208 SalI G'TCGA_C Cuts at: 1936 2088 3550 1936 Size: 152 1462 4594 Fragments arranged by size: 4594 1462 152 SanDI GG'GwC_CC Cuts at: 2275 3450 2275 Size: 1175 5033 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2748 2748 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 2260 2455 2518 2546 3344 3556 4691 Size: 219 1215 195 63 28 798 212 1135 Cuts at: 4691 5372 5563 826 Size: 681 191 1471 Fragments arranged by size: 1471 1215 1135 798 681 219 212 195 191 63 28 SfoI GGC'GCC Cuts at: 2049 2049 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 2436 3535 1872 Size: 564 1099 4545 Fragments arranged by size: 4545 1099 564 SmlI C'TyrA_G Cuts at: 820 1017 1943 4312 5213 5475 820 Size: 197 926 2369 901 262 1553 Fragments arranged by size: 2369 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3999 493 Size: 3506 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 3173 4132 5 Size: 47 3121 959 2081 Fragments arranged by size: 3121 2081 959 47 StuI AGG'CCT Cuts at: 1517 2556 1517 Size: 1039 5169 StyI C'CwwG_G Cuts at: 513 1065 2675 2949 3396 3647 513 Size: 552 1610 274 447 251 3074 Fragments arranged by size: 3074 1610 552 447 274 251 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3035 4235 4252 4405 4590 5035 325 Size: 1255 1455 1200 17 153 185 445 1498 Fragments arranged by size: 1498 1455 1255 1200 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 4454 6166 Size: 274 80 33 51 1443 258 2219 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 2219 1712 1443 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 6168 6200 2312 Size: 32 3824 32 2320 Fragments arranged by size: 3824 2320 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2156 1316 Size: 840 5368 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3147 3147 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6208 XmaI C'CCGG_G Cuts at: 1870 2434 3533 1870 Size: 564 1099 4545 Fragments arranged by size: 4545 1099 564 XmnI GAAnn'nnTTC Cuts at: 2105 4337 2105 Size: 2232 3976 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI