################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrDI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NspI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1174 GTCTAC GT^[AC][GT]AC AcyI 916 GGCGCC G[GA]^CG[CT]C AflIII 1196 ACACGT A^C[GA][CT]GT AlwI 147 GATCC GGATC(4/5) Aor51HI 1105 AGCGCT AGC^GCT BbeI 916 GGCGCC GGCGC^C BbrPI 811 CACGTG CAC^GTG BbvCI 104 CCTCAGC CCTCAGC(-5/-2) BsaHI 916 GGCGCC G[GA]^CG[CT]C BsaI 289 GAGACC GGTCTC(1/5) BsiEI 140 CGGCCG CG[GA][CT]^CG BsmBI 222 CGTCTC CGTCTC(1/5) BspHI 1121 TCATGA T^CATGA BssHII 1278 GCGCGC G^CGCGC BstAPI 777 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1155 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 140 CGGCCG C^GGCCG EclXI 140 CGGCCG C^GGCCG Eco47III 1105 AGCGCT AGC^GCT Eco52I 140 CGGCCG C^GGCCG EcoT22I 129 ATGCAT ATGCA^T Hin1I 916 GGCGCC G[GA]^CG[CT]C KasI 916 GGCGCC G^GCGCC NaeI 1043 GCCGGC GCC^GGC NarI 916 GGCGCC GG^CGCC NgoMIV 1043 GCCGGC G^CCGGC NruI 261 TCGCGA TCG^CGA NsiI 129 ATGCAT ATGCA^T PmaCI 811 CACGTG CAC^GTG PmlI 811 CACGTG CAC^GTG PshAI 594 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 523 CTGCAG CTGCA^G RcaI 1121 TCATGA T^CATGA SacI 396 GAGCTC GAGCT^C SexAI 1307 ACCAGGT A^CC[AT]GGT SfcI 523 CTGCAG C^T[GA][CT]AG SfiI 540 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 916 GGCGCC GGC^GCC TfiI 462 GAATC G^A[AT]TC TspRI 672 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### BanI 502 GGTGCC G^G[CT][GA]CC 916 GGCGCC BanII 396 GAGCTC G[GA]GC[CT]^C 991 GGGCTC BglI 541 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 908 GCCTCCCGGGC BsaAI 811 CACGTG [CT]AC^GT[GA] 1141 TACGTG BsgI 135 CTGCAC GTGCAG(16/14) 898 GTGCAG DpnII 147 GATC ^GATC 1125 GATC DraIII 811 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1306 CACCAGGTG EarI 67 CTCTTC CTCTTC(1/4) 82 GAAGAG HaeII 916 GGCGCC [GA]GCGC^[CT] 1105 AGCGCT Ksp632I 67 CTCTTC CTCTTC(1/4) 82 GAAGAG MboI 147 GATC ^GATC 1125 GATC MboII 82 GAAGA GAAGA(8/7) 68 TCTTC NcoI 233 CCATGG C^CATGG 626 CCATGG NdeII 147 GATC ^GATC 1125 GATC PaeR7I 170 CTCGAG C^TCGAG 1063 CTCGAG PflMI 228 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGAAGCTGG PleI 63 GACTC GAGTC(4/5) 183 GAGTC PvuII 94 CAGCTG CAG^CTG 326 CAGCTG Sau3AI 147 GATC ^GATC 1125 GATC SmaI 912 CCCGGG CCC^GGG 1271 CCCGGG StuI 48 AGGCCT AGG^CCT 1115 AGGCCT Tsp45I 1144 GTGAC ^GT[GC]AC 1324 GTCAC Van91I 228 CCAGTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCAGAAGCTGG XcmI 79 CCAGAAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1148 CCAACTCCCACCTGG XhoI 170 CTCGAG C^TCGAG 1063 CTCGAG XmaCI 912 CCCGGG C^CCGGG 1271 CCCGGG XmaI 912 CCCGGG C^CCGGG 1271 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI AvaII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI RsaI Sau96I ScrFI SfaNI SmlI SspBI StyI TaqI TseI TthHB8I