(Circular) (Six-base) MAPSORT of: pFN21AE6404.seq Check: 9956 from: 1 to: 6067 pFN21AE6404 6067 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2793 2793 Size: 6067 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3410 1937 Size: 1243 230 4594 Fragments arranged by size: 4594 1243 230 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2390 4130 5514 5782 1632 Size: 758 1740 1384 268 1917 Fragments arranged by size: 1917 1740 1384 758 268 AfeI AGC'GCT Cuts at: 3112 3112 Size: 6067 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 3201 4966 5943 1203 Size: 1998 1765 977 1327 Fragments arranged by size: 1998 1765 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 2147 3224 1166 Size: 981 1077 4009 Fragments arranged by size: 4009 1077 981 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2368 2581 2785 3810 5382 2368 Size: 213 204 1025 1572 3053 Fragments arranged by size: 3053 1572 1025 213 204 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3376 3602 3754 5863 1493 Size: 1883 226 152 2109 1697 Fragments arranged by size: 2109 1883 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2917 3068 3276 3392 1870 Size: 73 232 742 151 208 116 4545 Fragments arranged by size: 4545 742 232 208 151 116 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2507 2921 3388 4843 618 Size: 325 217 627 720 414 467 1455 1842 Fragments arranged by size: 1842 1455 720 627 467 414 325 217 BanII G_rGCy'C Cuts at: 1391 2405 3000 3386 1391 Size: 1014 595 386 4072 Fragments arranged by size: 4072 1014 595 386 BbeI G_GCGC'C Cuts at: 2925 2925 Size: 6067 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5096 BbvCI CC'TCA_GC Cuts at: 2110 2110 Size: 6067 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BfrBI ATG'CAT Cuts at: 2136 2136 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2552 2919 4678 Size: 107 122 71 631 605 880 367 1759 Cuts at: 4678 136 Size: 1525 Fragments arranged by size: 1759 1525 880 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2810 3231 4128 5284 1790 Size: 1020 421 897 1156 2573 Fragments arranged by size: 2573 1156 1020 897 421 BmgBI CAC'GTC Cuts at: 1318 3204 3226 3343 1318 Size: 1886 22 117 4042 Fragments arranged by size: 4042 1886 117 22 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2227 2670 3798 Size: 416 53 83 399 24 810 443 1128 Cuts at: 3798 4756 442 Size: 958 1753 Fragments arranged by size: 1753 1128 958 810 443 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2432 2570 2753 4727 1239 Size: 1019 174 138 183 1974 2579 Fragments arranged by size: 2579 1974 1019 183 174 138 Bpu10I CC'TnA_GC Cuts at: 2050 2110 2399 2050 Size: 60 289 5718 Fragments arranged by size: 5718 289 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2270 4192 5057 5355 2270 Size: 1922 865 298 2982 Fragments arranged by size: 2982 1922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2288 4730 882 Size: 617 789 2442 2219 Fragments arranged by size: 2442 2219 789 617 BsaAI yAC'GTr Cuts at: 493 1204 2818 3148 3858 493 Size: 711 1614 330 710 2702 Fragments arranged by size: 2702 1614 711 710 330 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2922 3874 4256 4891 Size: 53 83 186 936 1389 952 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1451 1389 952 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2816 2846 1178 Size: 30 165 30 1413 30 4399 Fragments arranged by size: 4399 1413 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 2513 2923 1186 Size: 1285 42 410 4330 Fragments arranged by size: 4330 1285 410 42 BseYI C'CCAG_C Cuts at: 2155 2581 2793 3167 5270 5766 2155 Size: 426 212 374 2103 496 2456 Fragments arranged by size: 2456 2103 496 426 374 212 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2125 2924 1163 Size: 573 389 799 4306 Fragments arranged by size: 4306 799 573 389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2148 3517 4278 5306 1540 Size: 275 186 147 1369 761 1028 2301 Fragments arranged by size: 2301 1369 1028 761 275 186 147 BsiHKAI G_wGCw'C Cuts at: 2051 2405 3144 3321 4128 4213 5284 2051 Size: 354 739 177 807 85 1071 2834 Fragments arranged by size: 2834 1071 807 739 354 177 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2233 2233 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2405 2810 3000 3144 3231 3321 Size: 399 261 354 405 190 144 87 90 Cuts at: 3321 3386 4128 4213 5284 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 354 261 190 144 90 87 85 65 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3126 3956 5686 3126 Size: 830 1730 3507 Fragments arranged by size: 3507 1730 830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2177 2535 2793 3422 844 Size: 1333 358 258 629 3489 Fragments arranged by size: 3489 1333 629 358 258 BsrBI CCG'CTC Cuts at: 1394 1823 2014 2886 2994 3954 1394 Size: 429 191 872 108 960 3507 Fragments arranged by size: 3507 960 872 429 191 108 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2924 3048 3092 4711 4898 1700 Size: 110 1114 124 44 1619 187 2869 Fragments arranged by size: 2869 1619 1114 187 124 110 44 BsrGI T'GTAC_A Cuts at: 96 2103 2823 3009 96 Size: 2007 720 186 3154 Fragments arranged by size: 3154 2007 720 186 BssHII G'CGCG_C Cuts at: 1925 3283 1925 Size: 1358 4709 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 2788 794 Size: 1994 4073 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstXI CCAn_nnnn'nTGG Cuts at: 3167 3167 Size: 6067 BstYI r'GATC_y Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062 Size: 1425 367 385 17 1452 86 12 346 Cuts at: 6062 1972 Size: 1977 Fragments arranged by size: 1977 1452 1425 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2238 2631 2798 513 Size: 552 1173 393 167 3782 Fragments arranged by size: 3782 1173 552 393 167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4377 4397 3583 Size: 794 20 5253 Fragments arranged by size: 5253 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DraIII CAC_nnn'GTG Cuts at: 2821 3316 2821 Size: 495 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2009 2058 2145 Size: 54 1425 50 275 18 179 49 87 Cuts at: 2145 2349 2487 3307 3514 4403 6032 8 Size: 204 138 820 207 889 1629 43 Fragments arranged by size: 1629 1425 889 820 275 207 204 179 138 87 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2145 3514 1537 Size: 275 333 1369 4090 Fragments arranged by size: 4090 1369 333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2082 3997 1353 Size: 725 4 1915 3423 Fragments arranged by size: 3423 1915 725 4 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2403 2403 Size: 6067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2390 2432 2570 2753 4130 4727 Size: 393 626 132 42 138 183 1377 597 Cuts at: 4727 5514 5782 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1377 787 626 597 393 268 183 138 132 42 EcoO109I rG'GnC_Cy Cuts at: 2119 2245 2340 2524 2637 2728 3361 2119 Size: 126 95 184 113 91 633 4825 Fragments arranged by size: 4825 633 184 126 113 95 91 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3199 3199 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 2925 3114 5214 1678 Size: 1247 189 2100 2531 Fragments arranged by size: 2531 2100 1247 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2055 2087 2742 2774 4708 Size: 32 135 32 483 32 655 32 1934 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1934 655 483 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KasI G'GCGC_C Cuts at: 2921 2921 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2991 5181 5365 1305 Size: 1686 2190 184 2007 Fragments arranged by size: 2190 2007 1686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 3224 3257 4025 4384 4543 518 Size: 648 981 1077 33 768 359 159 2042 Fragments arranged by size: 2042 1077 981 768 648 359 159 33 MspA1I CmG'CkG Cuts at: 1606 2032 2101 2333 3483 4160 5308 5553 1606 Size: 426 69 232 1150 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 677 426 245 232 69 NaeI GCC'GGC Cuts at: 3050 3050 Size: 6067 NarI GG'CG_CC Cuts at: 2922 2922 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 2238 2631 513 Size: 552 1173 393 3949 Fragments arranged by size: 3949 1173 552 393 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 3048 3048 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NruI TCG'CGA Cuts at: 2268 2268 Size: 6067 NsiI A_TGCA'T Cuts at: 2138 2138 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 2746 1348 Size: 1398 4669 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2581 1108 Size: 1131 342 4594 Fragments arranged by size: 4594 1131 342 PfoI T'CCnGG_A Cuts at: 2361 2409 2651 2730 2361 Size: 48 242 79 5698 Fragments arranged by size: 5698 242 79 48 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PmlI CAC'GTG Cuts at: 2818 2818 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2245 2340 2524 2637 2728 3361 2245 Size: 95 184 113 91 633 4951 Fragments arranged by size: 4951 633 184 113 95 91 PshAI GACnn'nnGTC Cuts at: 1613 2603 1613 Size: 990 5077 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspXI vC'TCGA_Gb Cuts at: 3068 3068 Size: 6067 PstI C_TGCA'G Cuts at: 830 2532 3419 830 Size: 1702 887 3478 Fragments arranged by size: 3478 1702 887 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2101 2333 2101 Size: 232 5835 SacI G_AGCT'C Cuts at: 2405 2405 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SanDI GG'GwC_CC Cuts at: 2340 2637 3361 2340 Size: 297 724 5046 Fragments arranged by size: 5046 724 297 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SexAI A'CCwGG_T Cuts at: 3312 3312 Size: 6067 SfcI C'TryA_G Cuts at: 826 1045 2528 3415 4550 5231 5422 826 Size: 219 1483 887 1135 681 191 1471 Fragments arranged by size: 1483 1471 1135 887 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2552 2552 Size: 6067 SfoI GGC'GCC Cuts at: 2923 2923 Size: 6067 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 2919 3278 3394 1872 Size: 1047 359 116 4545 Fragments arranged by size: 4545 1047 359 116 SmlI C'TyrA_G Cuts at: 820 1017 1943 2175 2249 3068 4171 5072 5334 Size: 197 926 232 74 819 1103 901 262 Cuts at: 5334 820 Size: 1553 Fragments arranged by size: 1553 1103 926 901 819 262 232 197 74 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 2055 3122 1517 Size: 538 1067 4462 Fragments arranged by size: 4462 1067 538 StyI C'CwwG_G Cuts at: 513 1065 2238 2631 3174 3506 513 Size: 552 1173 393 543 332 3074 Fragments arranged by size: 3074 1173 552 543 393 332 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 2495 4094 4111 4264 4449 4894 Size: 1255 815 100 1599 17 153 185 445 Cuts at: 4894 325 Size: 1498 Fragments arranged by size: 1599 1498 1255 815 445 185 153 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2103 2418 2823 Size: 274 80 33 51 1443 126 315 405 Cuts at: 2823 3009 3216 4313 6025 96 Size: 186 207 1097 1712 138 Fragments arranged by size: 1712 1443 1097 405 315 274 207 186 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2091 3160 2091 Size: 1069 4998 XhoI C'TCGA_G Cuts at: 1943 2175 3068 1943 Size: 232 893 4942 Fragments arranged by size: 4942 893 232 XmaI C'CCGG_G Cuts at: 1870 2917 3276 3392 1870 Size: 1047 359 116 4545 Fragments arranged by size: 4545 1047 359 116 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI PacI PspOMI PsrI RsrII SacII SapI SrfI SwaI