(Linear) (Six-base) MAPSORT of: KIEE6404.seq Check: 1762 from: 1 to: 1362 KIEE6404.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 788 1362 Size: 788 574 AccI GT'mk_AC Cuts at: 0 1175 1362 Size: 1175 187 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 1362 Size: 385 977 AfeI AGC'GCT Cuts at: 0 1107 1362 Size: 1107 255 AflIII A'CryG_T Cuts at: 0 1196 1362 Size: 1196 166 AleI CACnn'nnGTG Cuts at: 0 142 1219 1362 Size: 142 1077 143 Fragments arranged by size: 1077 143 142 AlwNI CAG_nnn'CTG Cuts at: 0 363 576 780 1362 Size: 363 213 204 582 Fragments arranged by size: 582 363 213 204 AvaI C'yCGr_G Cuts at: 0 170 912 1063 1271 1362 Size: 170 742 151 208 91 Fragments arranged by size: 742 208 170 151 91 BanI G'GyrC_C Cuts at: 0 502 916 1362 Size: 502 414 446 Fragments arranged by size: 502 446 414 BanII G_rGCy'C Cuts at: 0 400 995 1362 Size: 400 595 367 Fragments arranged by size: 595 400 367 BbeI G_GCGC'C Cuts at: 0 920 1362 Size: 920 442 BbvCI CC'TCA_GC Cuts at: 0 105 1362 Size: 105 1257 BfrBI ATG'CAT Cuts at: 0 131 1362 Size: 131 1231 BglI GCCn_nnn'nGGC Cuts at: 0 547 914 1362 Size: 547 367 448 Fragments arranged by size: 547 448 367 Bme1580I G_kGCm'C Cuts at: 0 805 1226 1362 Size: 805 421 136 Fragments arranged by size: 805 421 136 BmgBI CAC'GTC Cuts at: 0 1199 1221 1338 1362 Size: 1199 22 117 24 Fragments arranged by size: 1199 117 24 22 BmrI ACTGGGnnnn_n' Cuts at: 0 222 665 1362 Size: 222 443 697 Fragments arranged by size: 697 443 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 427 565 748 1362 Size: 253 174 138 183 614 Fragments arranged by size: 614 253 183 174 138 Bpu10I CC'TnA_GC Cuts at: 0 45 105 394 1362 Size: 45 60 289 968 Fragments arranged by size: 968 289 60 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 1362 Size: 265 1097 BsaI GGTCTCn'nnnn_ Cuts at: 0 283 1362 Size: 283 1079 BsaAI yAC'GTr Cuts at: 0 813 1143 1362 Size: 813 330 219 Fragments arranged by size: 813 330 219 BsaHI Gr'CG_yC Cuts at: 0 917 1362 Size: 917 445 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 811 841 1362 Size: 811 30 521 Fragments arranged by size: 811 521 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 466 508 918 1362 Size: 466 42 410 444 Fragments arranged by size: 466 444 410 42 BseYI C'CCAG_C Cuts at: 0 150 576 788 1162 1362 Size: 150 426 212 374 200 Fragments arranged by size: 426 374 212 200 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 919 1362 Size: 120 799 443 Fragments arranged by size: 799 443 120 BsiEI CG_ry'CG Cuts at: 0 143 1362 Size: 143 1219 BsiHKAI G_wGCw'C Cuts at: 0 46 400 1139 1316 1362 Size: 46 354 739 177 46 Fragments arranged by size: 739 354 177 46 46 BsmBI CGTCTCn'nnnn_ Cuts at: 0 228 1362 Size: 228 1134 Bsp1286I G_dGCh'C Cuts at: 0 46 400 805 995 1139 1226 1316 1362 Size: 46 354 405 190 144 87 90 46 Fragments arranged by size: 405 354 190 144 90 87 46 46 BspHI T'CATG_A Cuts at: 0 1121 1362 Size: 1121 241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 172 530 788 1362 Size: 172 358 258 574 Fragments arranged by size: 574 358 258 172 BsrBI CCG'CTC Cuts at: 0 9 881 989 1362 Size: 9 872 108 373 Fragments arranged by size: 872 373 108 9 BsrFI r'CCGG_y Cuts at: 0 919 1043 1087 1362 Size: 919 124 44 275 Fragments arranged by size: 919 275 124 44 BsrGI T'GTAC_A Cuts at: 0 98 818 1004 1362 Size: 98 720 186 358 Fragments arranged by size: 720 358 186 98 BssHII G'CGCG_C Cuts at: 0 1278 1362 Size: 1278 84 BstAPI GCAn_nnn'nTGC Cuts at: 0 783 1362 Size: 783 579 BstXI CCAn_nnnn'nTGG Cuts at: 0 1162 1362 Size: 1162 200 BtgI C'CryG_G Cuts at: 0 233 626 793 1362 Size: 233 393 167 569 Fragments arranged by size: 569 393 233 167 DraIII CAC_nnn'GTG Cuts at: 0 816 1311 1362 Size: 816 495 51 Fragments arranged by size: 816 495 51 EaeI y'GGCC_r Cuts at: 0 4 53 140 344 482 1302 1362 Size: 4 49 87 204 138 820 60 Fragments arranged by size: 820 204 138 87 60 49 4 EagI C'GGCC_G Cuts at: 0 140 1362 Size: 140 1222 EarI CTCTTCn'nnn_ Cuts at: 0 73 77 1362 Size: 73 4 1285 Fragments arranged by size: 1285 73 4 EcoICRI GAG'CTC Cuts at: 0 398 1362 Size: 398 964 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 385 427 565 748 1362 Size: 253 132 42 138 183 614 Fragments arranged by size: 614 253 183 138 132 42 EcoO109I rG'GnC_Cy Cuts at: 0 114 240 335 519 632 723 1356 1362 Size: 114 126 95 184 113 91 633 6 Fragments arranged by size: 633 184 126 114 113 95 91 6 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1194 1362 Size: 1194 168 HaeII r_GCGC'y Cuts at: 0 920 1109 1362 Size: 920 189 253 Fragments arranged by size: 920 253 189 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 50 82 737 769 1362 Size: 50 32 655 32 593 Fragments arranged by size: 655 593 50 32 32 KasI G'GCGC_C Cuts at: 0 916 1362 Size: 916 446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 986 1362 Size: 986 376 MslI CAynn'nnrTG Cuts at: 0 142 1219 1252 1362 Size: 142 1077 33 110 Fragments arranged by size: 1077 142 110 33 MspA1I CmG'CkG Cuts at: 0 27 96 328 1362 Size: 27 69 232 1034 Fragments arranged by size: 1034 232 69 27 NaeI GCC'GGC Cuts at: 0 1045 1362 Size: 1045 317 NarI GG'CG_CC Cuts at: 0 917 1362 Size: 917 445 NcoI C'CATG_G Cuts at: 0 233 626 1362 Size: 233 393 736 Fragments arranged by size: 736 393 233 NgoMIV G'CCGG_C Cuts at: 0 1043 1362 Size: 1043 319 NruI TCG'CGA Cuts at: 0 263 1362 Size: 263 1099 NsiI A_TGCA'T Cuts at: 0 133 1362 Size: 133 1229 PasI CC'CwG_GG Cuts at: 0 741 1362 Size: 741 621 PflMI CCAn_nnn'nTGG Cuts at: 0 234 576 1362 Size: 234 342 786 Fragments arranged by size: 786 342 234 PfoI T'CCnGG_A Cuts at: 0 356 404 646 725 1362 Size: 356 48 242 79 637 Fragments arranged by size: 637 356 242 79 48 PmlI CAC'GTG Cuts at: 0 813 1362 Size: 813 549 PpuMI rG'GwC_Cy Cuts at: 0 240 335 519 632 723 1356 1362 Size: 240 95 184 113 91 633 6 Fragments arranged by size: 633 240 184 113 95 91 6 PshAI GACnn'nnGTC Cuts at: 0 598 1362 Size: 598 764 PspXI vC'TCGA_Gb Cuts at: 0 1063 1362 Size: 1063 299 PstI C_TGCA'G Cuts at: 0 527 1362 Size: 527 835 PvuII CAG'CTG Cuts at: 0 96 328 1362 Size: 96 232 1034 Fragments arranged by size: 1034 232 96 SacI G_AGCT'C Cuts at: 0 400 1362 Size: 400 962 SanDI GG'GwC_CC Cuts at: 0 335 632 1356 1362 Size: 335 297 724 6 Fragments arranged by size: 724 335 297 6 SexAI A'CCwGG_T Cuts at: 0 1307 1362 Size: 1307 55 SfcI C'TryA_G Cuts at: 0 523 1362 Size: 523 839 SfiI GGCCn_nnn'nGGCC Cuts at: 0 547 1362 Size: 547 815 SfoI GGC'GCC Cuts at: 0 918 1362 Size: 918 444 SmaI CCC'GGG Cuts at: 0 914 1273 1362 Size: 914 359 89 Fragments arranged by size: 914 359 89 SmlI C'TyrA_G Cuts at: 0 170 244 1063 1362 Size: 170 74 819 299 Fragments arranged by size: 819 299 170 74 StuI AGG'CCT Cuts at: 0 50 1117 1362 Size: 50 1067 245 Fragments arranged by size: 1067 245 50 StyI C'CwwG_G Cuts at: 0 233 626 1169 1362 Size: 233 393 543 193 Fragments arranged by size: 543 393 233 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 390 490 1362 Size: 390 100 872 Fragments arranged by size: 872 390 100 TatI w'GTAC_w Cuts at: 0 98 413 818 1004 1211 1362 Size: 98 315 405 186 207 151 Fragments arranged by size: 405 315 207 186 151 98 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 86 1155 1362 Size: 86 1069 207 Fragments arranged by size: 1069 207 86 XhoI C'TCGA_G Cuts at: 0 170 1063 1362 Size: 170 893 299 Fragments arranged by size: 893 299 170 XmaI C'CCGG_G Cuts at: 0 912 1271 1362 Size: 912 359 91 Fragments arranged by size: 912 359 91 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AleI AlwNI AvaI BanI BanII BbeI BbvCI BfrBI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BtgI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI PasI PflMI PfoI PmlI PpuMI PshAI PspXI PstI PvuII SacI SanDI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BglII BlpI BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI BsrDI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NspI PacI PciI PmeI PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmnI ZraI