Restriction map for
pFN21AB9452
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaBI BseAI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI Eco47III Eco81I EcoO65I EcoRV
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1174 GTCTAC GT^[AC][GT]AC
AhdI 1345 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1110 GGGCCC GGGCC^C
AspEI 1345 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1168 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 632 GGCGCC GGCGC^C
BlpI 1406 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1406 GCTCAGC GC^T[ACGT]AGC
BsaAI 1552 TACGTG [CT]AC^GT[GA]
BsaI 559 GAGACC GGTCTC(1/5)
BsaWI 740 ACCGGA [AT]^CCGG[AT]
BsiEI 14 CGGCCG CG[GA][CT]^CG
BsiWI 1442 CGTACG C^GTACG
BsmBI 144 CGTCTC CGTCTC(1/5)
BspHI 1358 TCATGA T^CATGA
BsrDI 1271 GCAATG GCAATG(2/0)
BsrFI 1008 GCCGGT [GA]^CCGG[CT]
BstAPI 68 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 170 CCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1406 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1008 GCCGGT [GA]^CCGG[CT]
DraIII 214 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 14 CGGCCG [CT]^GGCC[GA]
EagI 14 CGGCCG C^GGCCG
Eam1105I 1345 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 14 CGGCCG C^GGCCG
Eco52I 14 CGGCCG C^GGCCG
EcoNI 1095 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1287 GAATTC G^AATTC
EcoT22I 1087 ATGCAT ATGCA^T
FauI 57 GCGGG CCCGC(4/6)
HaeII 632 GGCGCC [GA]GCGC^[CT]
HincII 655 GTCAAC GT[CT]^[GA]AC
HindII 655 GTCAAC GT[CT]^[GA]AC
KasI 632 GGCGCC G^GCGCC
MslI 1267 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1435 CAGCTG C[AC]G^C[GT]G
NarI 632 GGCGCC GG^CGCC
NsiI 1087 ATGCAT ATGCA^T
PflFI 1168 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 387 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 200 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 775 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 698 CTGCAG CTGCA^G
PvuII 1435 CAGCTG CAG^CTG
RcaI 1358 TCATGA T^CATGA
SacI 1336 GAGCTC GAGCT^C
ScaI 389 AGTACT AGT^ACT
SfoI 632 GGCGCC GGC^GCC
SmaI 87 CCCGGG CCC^GGG
Tth111I 1168 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 387 CCAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 364 AAATTT [GA]^AATT[CT]
1287 GAATTC
ApoI 364 AAATTT [GA]^AATT[CT]
1287 GAATTC
AvaI 87 CCCGGG C^[CT]CG[GA]G
460 CCCGAG
BbsI 1062 GAAGAC GAAGAC(2/6)
1456 GAAGAC
BbvCI 51 GCTGAGG CCTCAGC(-5/-2)
759 GCTGAGG
BpmI 178 CTGGAG CTGGAG(16/14)
691 CTGGAG
BpuAI 1062 GAAGAC GAAGAC(2/6)
1456 GAAGAC
BsgI 723 CTGCAC GTGCAG(16/14)
1293 CTGCAC
BsoBI 87 CCCGGG C^[CT]CG[GA]G
460 CCCGAG
BsrBI 493 CCGCTC CCGCTC(-3/-3)
1377 CCGCTC
BstYI 152 GGATCT [GA]^GATC[CT]
335 AGATCC
DrdI 1003 GACTTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1440 GACGTACGAGTC
DsaI 44 CCGTGG C^C[GA][CT]GG
734 CCGTGG
MflI 152 GGATCT [GA]^GATC[CT]
335 AGATCC
SfcI 698 CTGCAG C^T[GA][CT]AG
1038 CTACAG
SmlI 838 CTCAAG C^T[CT][GA]AG
1162 CTCAAG
StuI 470 AGGCCT AGG^CCT
1387 AGGCCT
TaqI 903 TCGA T^CGA
956 TCGA
Tsp509I 365 AATT ^AATT
1288 AATT
TthHB8I 903 TCGA T^CGA
956 TCGA
XcmI 167 CCACCAAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
680 CCAAGCAGCCACTGG
XhoII 152 GGATCT [GA]^GATC[CT]
335 AGATCC
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI TseI
Tsp45I TspRI