################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BseRI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AlwI 191 GGATC GGATC(4/5) ApaI 684 GGGCCC GGGCC^C AvaI 40 CTCGAG C^[CT]CG[GA]G AvaII 873 GGACC G^G[AT]CC BbsI 423 GTCTTC GAAGAC(2/6) BcnI 132 CCCGG CC^[GC]GG BglI 430 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 165 GCTGAGC GC^T[ACGT]AGC BpmI 637 CTCCAG CTGGAG(16/14) Bpu1102I 165 GCTGAGC GC^T[ACGT]AGC BpuAI 423 GTCTTC GAAGAC(2/6) BsaWI 85 TCCGGT [AT]^CCGG[AT] BsmBI 396 CGTCTC CGTCTC(1/5) BsmI 201 GAATGC GAATGC(1/-1) BsoBI 40 CTCGAG C^[CT]CG[GA]G BsrFI 25 ACCGGC [GA]^CCGG[CT] BstAPI 750 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 165 GCTGAGC GC^T[ACGT]AGC CfoI 249 GCGC GCG^C Cfr10I 25 ACCGGC [GA]^CCGG[CT] EcoT14I 512 CCAAGG C^C[AT][AT]GG FauI 760 CCCGC CCCGC(4/6) HaeII 248 GGCGCT [GA]GCGC^[CT] HgaI 666 GACGC GACGC(5/10) HhaI 249 GCGC GCG^C HinP1I 249 GCGC G^CGC MaeII 725 ACGT A^CGT MspA1I 376 CAGCTG C[AC]G^C[GT]G NciI 132 CCCGG CC^[GC]GG NspI 490 ACATGC [GA]CATG^[CT] PaeR7I 40 CTCGAG C^TCGAG PleI 144 GACTC GAGTC(4/5) PvuII 376 CAGCTG CAG^CTG SacI 37 GAGCTC GAGCT^C SapI 408 GCTCTTC GCTCTTC(1/4) SfaNI 317 GCATC GCATC(5/9) SmlI 40 CTCGAG C^T[CT][GA]AG StyI 512 CCAAGG C^C[AT][AT]GG TaqI 41 TCGA T^CGA TfiI 836 GAATC G^A[AT]TC TthHB8I 41 TCGA T^CGA XcmI 743 CCAAACTGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 40 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 37 GAGCTC G[AT]GC[AT]^C 406 GTGCTC BalI 311 TGGCCA TGG^CCA 630 TGGCCA BanI 756 GGTGCC G^G[CT][GA]CC 780 GGTGCC BanII 37 GAGCTC G[GA]GC[CT]^C 684 GGGCCC BsaI 7 GAGACC GGTCTC(1/5) 88 GGTCTC BsiHKAI 37 GAGCTC G[AT]GC[AT]^C 406 GTGCTC BsmFI 487 GGGAC GGGAC(10/14) 518 GGGAC BsrDI 768 CATTGC GCAATG(2/0) 815 GCAATG DraII 683 GGGGCCC [GA]G^G[ACGT]CC[CT] 684 GGGCCCC EaeI 311 TGGCCA [CT]^GGCC[GA] 630 TGGCCA EcoO109I 683 GGGGCCC [GA]G^G[ACGT]CC[CT] 684 GGGCCCC HinfI 144 GACTC G^A[ACGT]TC 836 GAATC MluNI 311 TGGCCA TGG^CCA 630 TGGCCA MscI 311 TGGCCA TGG^CCA 630 TGGCCA PflMI 153 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 335 CCATCGCATGG Van91I 153 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 335 CCATCGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwNI BbvI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI