(Circular) (Six-base) MAPSORT of: pFN21AE9531.seq Check: 918 from: 1 to: 5590 pFN21AE9531 5590 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2933 1937 Size: 996 4594 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 2256 2325 2623 3333 4905 2256 Size: 69 298 710 1572 2941 Fragments arranged by size: 2941 1572 710 298 69 ApaI G_GGCC'C Cuts at: 2693 2693 Size: 5590 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2045 2915 1870 Size: 73 102 870 4545 Fragments arranged by size: 4545 870 102 73 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2761 2785 2911 4366 618 Size: 325 217 627 974 24 126 1455 1842 Fragments arranged by size: 1842 1455 974 627 325 217 126 24 BanII G_rGCy'C Cuts at: 1391 2046 2693 2909 1391 Size: 655 647 216 4072 Fragments arranged by size: 4072 655 647 216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2421 928 Size: 971 522 4097 Fragments arranged by size: 4097 971 522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2441 4201 136 Size: 107 122 71 631 605 769 1760 1525 Fragments arranged by size: 1760 1525 769 631 605 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 2171 3007 2171 Size: 836 4754 Bme1580I G_kGCm'C Cuts at: 1790 2693 2766 2790 3651 4807 1790 Size: 903 73 24 861 1156 2573 Fragments arranged by size: 2573 1156 903 861 73 24 BmgBI CAC'GTC Cuts at: 1318 2731 1318 Size: 1413 4177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2498 2633 3321 Size: 416 53 83 399 24 1081 135 688 Cuts at: 3321 4279 442 Size: 958 1753 Fragments arranged by size: 1753 1081 958 688 416 399 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2196 2196 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2627 4250 1239 Size: 1388 1623 2579 Fragments arranged by size: 2579 1623 1388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2006 2099 4253 882 Size: 617 507 93 2154 2219 Fragments arranged by size: 2219 2154 617 507 93 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2090 4023 4421 4695 4842 5253 2090 Size: 1933 398 274 147 411 2427 Fragments arranged by size: 2427 1933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5590 BseYI C'CCAG_C Cuts at: 2685 4793 5289 2685 Size: 2108 496 2986 Fragments arranged by size: 2986 2108 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 2355 2485 1163 Size: 573 617 2 130 4268 Fragments arranged by size: 4268 617 573 130 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2415 3651 3736 4807 2046 Size: 369 1236 85 1071 2829 Fragments arranged by size: 2829 1236 1071 369 85 BsmI GAATG_Cn' Cuts at: 2212 3101 3194 2212 Size: 889 93 4608 Fragments arranged by size: 4608 889 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2407 2407 Size: 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2415 2693 2766 2790 2909 3651 Size: 399 256 369 278 73 24 119 742 Cuts at: 3651 3736 4807 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 278 256 119 85 73 24 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 2827 4085 4259 65 Size: 1183 1524 55 1258 174 1396 Fragments arranged by size: 1524 1396 1258 1183 174 55 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 4234 4421 1700 Size: 110 220 2204 187 2869 Fragments arranged by size: 2869 2204 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 2761 794 Size: 1967 3623 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2326 2707 487 Size: 683 56 1100 381 3370 Fragments arranged by size: 3370 1100 683 381 56 BtsI GCAGTG_nn' Cuts at: 2338 2855 3106 3900 3920 2338 Size: 517 251 794 20 4008 Fragments arranged by size: 4008 794 517 251 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2635 3037 Size: 54 1425 50 275 18 486 319 402 Cuts at: 3037 3926 5555 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 486 402 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2420 2552 2588 2624 2861 3520 1353 Size: 1067 132 36 36 237 659 3423 Fragments arranged by size: 3423 1067 659 237 132 36 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 5590 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 3653 4250 5037 5305 1239 Size: 393 995 1026 597 787 268 1524 Fragments arranged by size: 1524 1026 995 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2689 2690 2689 Size: 1 5589 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2257 4737 1678 Size: 579 2480 2531 Fragments arranged by size: 2531 2480 579 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2623 2655 4231 4263 4305 Size: 32 135 32 1051 32 1576 32 42 Cuts at: 4305 4337 1373 Size: 32 2626 Fragments arranged by size: 2626 1576 1051 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 2637 10 Size: 54 1425 343 486 319 2963 Fragments arranged by size: 2963 1425 486 343 319 54 MslI CAynn'nnrTG Cuts at: 518 1166 3548 3907 4066 518 Size: 648 2382 359 159 2042 Fragments arranged by size: 2382 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2383 3006 3683 4831 5076 1606 Size: 777 623 677 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 623 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2499 2948 4493 1132 Size: 97 1270 449 1545 2229 Fragments arranged by size: 2229 1545 1270 449 97 PasI CC'CwG_GG Cuts at: 1348 2815 1348 Size: 1467 4123 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2164 2346 1108 Size: 1056 182 4352 Fragments arranged by size: 4352 1056 182 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PspOMI G'GGCC_C Cuts at: 2689 2689 Size: 5590 PspXI vC'TCGA_Gb Cuts at: 2045 2045 Size: 5590 PstI C_TGCA'G Cuts at: 830 2330 2757 2837 2942 830 Size: 1500 427 80 105 3478 Fragments arranged by size: 3478 1500 427 105 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 PvuII CAG'CTG Cuts at: 2383 2383 Size: 5590 SacI G_AGCT'C Cuts at: 2046 2046 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SapI GCTCTTCn'nnn_ Cuts at: 2420 2420 Size: 5590 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2326 2602 2753 2833 2938 4073 4754 Size: 219 1281 276 151 80 105 1135 681 Cuts at: 4754 4945 826 Size: 191 1471 Fragments arranged by size: 1471 1281 1135 681 276 219 191 151 105 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 3694 4595 4857 820 Size: 197 926 102 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 2886 3029 513 Size: 552 939 513 369 143 3074 Fragments arranged by size: 3074 939 552 513 369 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2895 3617 3634 3787 3972 4417 325 Size: 1255 1315 722 17 153 185 445 1498 Fragments arranged by size: 1498 1315 1255 722 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5550 5582 5550 Size: 32 5558 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2755 2755 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 2045 1943 Size: 102 5488 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI