(Circular) (Six-base) MAPSORT of: pFN21AB5057.seq Check: 4217 from: 1 to: 6304 pFN21AB5057 6304 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2080 2080 Size: 6304 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4114 278 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3647 1937 Size: 1710 4594 Acc65I G'GTAC_C Cuts at: 3625 3625 Size: 6304 AclI AA'CG_TT Cuts at: 1563 4431 4804 1563 Size: 2868 373 3063 Fragments arranged by size: 3063 2868 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3113 3346 3603 4367 5751 6019 1632 Size: 1481 233 257 764 1384 268 1917 Fragments arranged by size: 1917 1481 1384 764 268 257 233 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6107 AflIII A'CryG_T Cuts at: 1203 2784 5203 6180 1203 Size: 1581 2419 977 1327 Fragments arranged by size: 2419 1581 1327 977 AgeI A'CCGG_T Cuts at: 5135 5135 Size: 6304 AhdI GACnn_n'nnGTC Cuts at: 5033 5033 Size: 6304 AleI CACnn'nnGTG Cuts at: 1166 2177 2240 2856 1166 Size: 1011 63 616 4614 Fragments arranged by size: 4614 1011 616 63 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3407 1184 Size: 2223 4081 AlwNI CAG_nnn'CTG Cuts at: 2575 4047 5619 2575 Size: 1472 1572 3260 Fragments arranged by size: 3260 1572 1472 ApaI G_GGCC'C Cuts at: 2749 2749 Size: 6304 ApaLI G'TGCA_C Cuts at: 2618 4361 5517 2618 Size: 1743 1156 3405 Fragments arranged by size: 3405 1743 1156 ApoI r'AATT_y Cuts at: 1493 2451 3613 3839 3991 6100 1493 Size: 958 1162 226 152 2109 1697 Fragments arranged by size: 2109 1697 1162 958 226 152 AseI AT'TA_AT Cuts at: 160 1032 4858 160 Size: 872 3826 1606 Fragments arranged by size: 3826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 AvaI C'yCGr_G Cuts at: 1870 1943 3629 1870 Size: 73 1686 4545 Fragments arranged by size: 4545 1686 73 BamHI G'GATC_C Cuts at: 3634 4001 3634 Size: 367 5937 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2732 2858 3290 3625 5080 Size: 325 217 627 945 126 432 335 1455 Cuts at: 5080 618 Size: 1842 Fragments arranged by size: 1842 1455 945 627 432 335 325 217 126 BanII G_rGCy'C Cuts at: 1391 2749 3348 3623 1391 Size: 1358 599 275 4072 Fragments arranged by size: 4072 1358 599 275 BbeI G_GCGC'C Cuts at: 2736 3294 2736 Size: 558 5746 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2519 3352 928 Size: 971 620 833 3880 Fragments arranged by size: 3880 971 833 620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2632 2666 4495 4529 1903 Size: 34 695 34 1829 34 3678 Fragments arranged by size: 3678 1829 695 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 5406 4196 Size: 1210 5094 BclI T'GATC_A Cuts at: 1548 2343 1548 Size: 795 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4915 136 Size: 107 122 71 631 605 3243 1525 Fragments arranged by size: 3243 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 2589 6299 2067 Size: 522 3710 2072 Fragments arranged by size: 3710 2072 522 BlpI GC'TnA_GC Cuts at: 3183 3721 3183 Size: 538 5766 Bme1580I G_kGCm'C Cuts at: 1790 2622 2710 2749 4365 5521 1790 Size: 832 88 39 1616 1156 2573 Fragments arranged by size: 2573 1616 1156 832 88 39 BmgBI CAC'GTC Cuts at: 1318 2785 3526 1318 Size: 1467 741 4096 Fragments arranged by size: 4096 1467 741 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2668 4035 4993 Size: 416 53 83 399 24 1251 1367 958 Cuts at: 4993 442 Size: 1753 Fragments arranged by size: 1753 1367 1251 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2279 2603 4964 1239 Size: 959 81 324 2361 2579 Fragments arranged by size: 2579 2361 959 324 81 Bpu10I CC'TnA_GC Cuts at: 2111 3113 2111 Size: 1002 5302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4429 5294 5592 4429 Size: 865 298 5141 Fragments arranged by size: 5141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2554 3509 4967 882 Size: 617 1055 955 1458 2219 Fragments arranged by size: 2219 1458 1055 955 617 BsaAI yAC'GTr Cuts at: 493 1204 4095 493 Size: 711 2891 2702 Fragments arranged by size: 2891 2702 711 BsaBI GATnn'nnATC Cuts at: 6298 6298 Size: 6304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2733 3291 4111 4493 Size: 53 83 186 936 1200 558 820 382 Cuts at: 4493 5128 275 Size: 635 1451 Fragments arranged by size: 1451 1200 936 820 635 558 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3408 4737 5135 5409 5556 5967 3408 Size: 1329 398 274 147 411 3745 Fragments arranged by size: 3745 1329 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2706 2736 1178 Size: 30 165 30 1303 30 4746 Fragments arranged by size: 4746 1303 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2600 2616 3529 1186 Size: 949 465 16 913 3961 Fragments arranged by size: 3961 949 913 465 16 BseYI C'CCAG_C Cuts at: 3044 3101 3551 5507 6003 3044 Size: 57 450 1956 496 3345 Fragments arranged by size: 3345 1956 496 450 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2760 3222 1163 Size: 573 1024 462 4245 Fragments arranged by size: 4245 1024 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3754 4515 5543 1540 Size: 275 186 1753 761 1028 2301 Fragments arranged by size: 2301 1753 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 2622 4365 4450 5521 2034 Size: 588 1743 85 1071 2817 Fragments arranged by size: 2817 1743 1071 588 85 BsmI GAATG_Cn' Cuts at: 2959 3103 3269 3815 3908 2959 Size: 144 166 546 93 5355 Fragments arranged by size: 5355 546 166 144 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2622 2710 2749 3348 3623 4365 Size: 399 244 588 88 39 599 275 742 Cuts at: 4365 4450 5521 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 599 588 399 275 244 88 85 39 BspEI T'CCGG_A Cuts at: 3408 5967 3408 Size: 2559 3745 BspHI T'CATG_A Cuts at: 4193 5923 4193 Size: 1730 4574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2080 3399 3659 844 Size: 1236 1319 260 3489 Fragments arranged by size: 3489 1319 1236 260 BsrBI CCG'CTC Cuts at: 1394 1823 2407 4191 1394 Size: 429 584 1784 3507 Fragments arranged by size: 3507 1784 584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 4799 4973 65 Size: 1183 2190 1361 174 1396 Fragments arranged by size: 2190 1396 1361 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4948 5135 1700 Size: 110 3138 187 2869 Fragments arranged by size: 3138 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6304 BssSI C'ACGA_G Cuts at: 4364 5376 4364 Size: 1012 5292 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6304 BstBI TT'CG_AA Cuts at: 5108 5108 Size: 6304 BstEII G'GTnAC_C Cuts at: 1184 2179 1184 Size: 995 5309 BstYI r'GATC_y Cuts at: 1972 2067 2254 2589 3634 4001 4386 4403 5855 Size: 95 187 335 1045 367 385 17 1452 Cuts at: 5855 5941 5953 6299 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1045 385 367 346 335 187 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2710 2804 3276 513 Size: 552 939 706 94 472 3541 Fragments arranged by size: 3541 939 706 552 472 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5565 Fragments arranged by size: 5565 683 56 BtsI GCAGTG_nn' Cuts at: 3820 4614 4634 3820 Size: 794 20 5490 Fragments arranged by size: 5490 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6304 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6269 DraI TTT'AAA Cuts at: 3608 3608 Size: 6304 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5311 809 Size: 507 3995 1802 Fragments arranged by size: 3995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2355 2758 3273 Size: 54 1425 50 275 18 525 403 515 Cuts at: 3273 3751 4640 6269 8 Size: 478 889 1629 43 Fragments arranged by size: 1629 1425 889 525 515 478 403 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3751 1537 Size: 275 1939 4090 Fragments arranged by size: 4090 1939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2790 4234 1353 Size: 1437 1444 3423 Fragments arranged by size: 3423 1444 1437 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3176 4891 5265 5411 387 Size: 1064 138 1587 1715 374 146 1280 Fragments arranged by size: 1715 1587 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2279 2603 3113 3346 3603 4367 Size: 393 566 81 324 510 233 257 764 Cuts at: 4367 4964 5751 6019 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 764 597 566 510 393 324 268 257 233 81 EcoNI CCTnn'n_nnAGG Cuts at: 3143 3461 3143 Size: 318 5986 EcoO109I rG'GnC_Cy Cuts at: 2746 2962 3048 3388 2746 Size: 216 86 340 5662 Fragments arranged by size: 5662 340 216 86 EcoRI G'AATT_C Cuts at: 2451 3613 3991 2451 Size: 1162 378 4764 Fragments arranged by size: 4764 1162 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2299 2299 Size: 6304 FspI TGC'GCA Cuts at: 4810 4810 Size: 6304 HaeII r_GCGC'y Cuts at: 1678 2736 3294 5451 1678 Size: 1058 558 2157 2531 Fragments arranged by size: 2531 2157 1058 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 4945 4977 5019 Size: 32 135 32 1009 32 2332 32 42 Cuts at: 5019 5051 1373 Size: 32 2626 Fragments arranged by size: 2626 2332 1009 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3648 3894 669 Size: 988 281 1710 246 3079 Fragments arranged by size: 3079 1710 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6304 HpaI GTT'AAC Cuts at: 3894 3894 Size: 6304 KasI G'GCGC_C Cuts at: 2732 3290 2732 Size: 558 5746 KpnI G_GTAC'C Cuts at: 3629 3629 Size: 6304 MfeI C'AATT_G Cuts at: 3903 3903 Size: 6304 MluI A'CGCG_T Cuts at: 6180 6180 Size: 6304 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5418 5602 1305 Size: 4113 184 2007 Fragments arranged by size: 4113 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2357 2760 10 Size: 54 1425 343 525 403 3554 Fragments arranged by size: 3554 1425 525 403 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2240 2615 2832 2856 3068 4262 Size: 648 1011 63 375 217 24 212 1194 Cuts at: 4262 4621 4780 518 Size: 359 159 2042 Fragments arranged by size: 2042 1194 1011 648 375 359 217 212 159 63 24 MspA1I CmG'CkG Cuts at: 1606 2186 2204 2575 2844 2886 3720 4397 5545 Size: 580 18 371 269 42 834 677 1148 Cuts at: 5545 5790 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 834 677 580 371 269 245 42 18 NarI GG'CG_CC Cuts at: 2733 3291 2733 Size: 558 5746 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4813 Fragments arranged by size: 4813 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6304 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6304 NotI GC'GGCC_GC Cuts at: 3751 3751 Size: 6304 NspI r_CATG'y Cuts at: 1132 1229 3578 3662 5207 1132 Size: 97 2349 84 1545 2229 Fragments arranged by size: 2349 2229 1545 97 84 PasI CC'CwG_GG Cuts at: 1348 2446 1348 Size: 1098 5206 PciI A'CATG_T Cuts at: 5203 5203 Size: 6304 PflMI CCAn_nnn'nTGG Cuts at: 1108 2221 2997 3307 1108 Size: 1113 776 310 4105 Fragments arranged by size: 4105 1113 776 310 PmeI GTTT'AAAC Cuts at: 3608 3608 Size: 6304 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4373 4405 5886 5918 4373 Size: 32 1481 32 4759 Fragments arranged by size: 4759 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3048 3388 3048 Size: 340 5964 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6304 PsiI TTA'TAA Cuts at: 3874 3874 Size: 6304 PspOMI G'GGCC_C Cuts at: 2745 2745 Size: 6304 PstI C_TGCA'G Cuts at: 830 2091 2142 2754 3342 3656 830 Size: 1261 51 612 588 314 3478 Fragments arranged by size: 3478 1261 612 588 314 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6304 PvuII CAG'CTG Cuts at: 2204 2575 2844 2204 Size: 371 269 5664 Fragments arranged by size: 5664 371 269 SalI G'TCGA_C Cuts at: 1936 3646 1936 Size: 1710 4594 SanDI GG'GwC_CC Cuts at: 3048 3048 Size: 6304 SapI GCTCTTCn'nnn_ Cuts at: 2790 2790 Size: 6304 SbfI CC_TGCA'GG Cuts at: 3656 3656 Size: 6304 ScaI AGT'ACT Cuts at: 4552 6264 4552 Size: 1712 4592 SfcI C'TryA_G Cuts at: 826 1045 2087 2138 2517 2750 3338 3652 4787 Size: 219 1042 51 379 233 588 314 1135 Cuts at: 4787 5468 5659 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 588 379 314 233 219 191 51 SfoI GGC'GCC Cuts at: 2734 3292 2734 Size: 558 5746 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6304 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6304 SmaI CCC'GGG Cuts at: 1872 3631 1872 Size: 1759 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4408 5309 5571 820 Size: 197 926 2465 901 262 1553 Fragments arranged by size: 2465 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4095 493 Size: 3602 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6304 SphI G_CATG'C Cuts at: 1132 3662 1132 Size: 2530 3774 SspI AAT'ATT Cuts at: 5 52 4228 5 Size: 47 4176 2081 Fragments arranged by size: 4176 2081 47 StuI AGG'CCT Cuts at: 1517 2154 1517 Size: 637 5667 StyI C'CwwG_G Cuts at: 513 1065 2004 2175 2817 2868 2996 3319 3743 Size: 552 939 171 642 51 128 323 424 Cuts at: 3743 513 Size: 3074 Fragments arranged by size: 3074 939 642 552 424 323 171 128 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2846 4331 4348 4501 4686 5131 325 Size: 1255 1266 1485 17 153 185 445 1498 Fragments arranged by size: 1498 1485 1266 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3334 4550 6262 Size: 274 80 33 51 1443 1357 1216 1712 Cuts at: 6262 96 Size: 138 Fragments arranged by size: 1712 1443 1357 1216 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2706 2738 6264 6296 2706 Size: 32 3526 32 2714 Fragments arranged by size: 3526 2714 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6304 XbaI T'CTAG_A Cuts at: 3640 3640 Size: 6304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6304 XmaI C'CCGG_G Cuts at: 1870 3629 1870 Size: 1759 4545 XmnI GAAnn'nnTTC Cuts at: 2414 4433 2414 Size: 2019 4285 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4112 276 Size: 53 83 186 936 2578 2468 Fragments arranged by size: 2578 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI