################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI NarI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 301 GTCTAC GT^[AC][GT]AC AciI 223 GCGG CCGC(-3/-1) AfaI 183 GTAC GT^AC AluI 278 AGCT AG^CT AlwI 134 GGATC GGATC(4/5) AspI 258 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 201 GGTCC G^G[AT]CC BpmI 280 CTCCAG CTGGAG(16/14) BsrDI 168 CATTGC GCAATG(2/0) BsrFI 194 GCCGGC [GA]^CCGG[CT] BstF5I 297 GGATG GGATG(2/0) Cfr10I 194 GCCGGC [GA]^CCGG[CT] DdeI 254 CTGAG C^T[ACGT]AG DpnII 135 GATC ^GATC EaeI 176 TGGCCG [CT]^GGCC[GA] EcoNI 17 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 297 GGATG GGATG(9/13) HinfI 262 GAGTC G^A[ACGT]TC HphI 111 GGTGA GGTGA(8/7) MboI 135 GATC ^GATC NaeI 194 GCCGGC GCC^GGC NdeII 135 GATC ^GATC NgoMIV 194 GCCGGC G^CCGGC PflFI 258 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 262 GAGTC GAGTC(4/5) PstI 18 CTGCAG CTGCA^G RsaI 183 GTAC GT^AC SacI 277 GAGCTC GAGCT^C Sau3AI 135 GATC ^GATC SexAI 149 ACCTGGT A^CC[AT]GGT SmaI 117 CCCGGG CCC^GGG Tth111I 258 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 117 CCCGGG C^CCGGG XmaI 117 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AspHI 163 GTGCTC G[AT]GC[AT]^C 277 GAGCTC AvaI 117 CCCGGG C^[CT]CG[GA]G 206 CTCGGG BanII 114 GAGCCC G[GA]GC[CT]^C 277 GAGCTC BsiHKAI 163 GTGCTC G[AT]GC[AT]^C 277 GAGCTC BsiYI 17 CCTGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCAGGCCCAGG BslI 17 CCTGCAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 75 CCAGGCCCAGG BsmFI 6 GGGAC GGGAC(10/14) 202 GTCCC BsoBI 117 CCCGGG C^[CT]CG[GA]G 206 CTCGGG NlaIV 26 GGCTCC GG[ACGT]^[ACGT]CC 201 GGTCCC SfcI 18 CTGCAG C^T[GA][CT]AG 303 CTACAG TspRI 161 CAGTG CA[GC]TG[ACGT][ACGT]^ 252 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### BbvI BcnI BmyI BsaI BsaJI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NciI Sau96I ScrFI TseI