(Circular) (Six-base) MAPSORT of: pFN21AE1037.seq Check: 4033 from: 1 to: 5866 pFN21AE1037 5866 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3165 3993 4366 1563 Size: 1602 828 373 3063 Fragments arranged by size: 3063 1602 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 2966 3929 5313 5581 1632 Size: 665 669 963 1384 268 1917 Fragments arranged by size: 1917 1384 963 669 665 268 AflII C'TTAA_G Cuts at: 820 1017 3139 820 Size: 197 2122 3547 Fragments arranged by size: 3547 2122 197 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 3071 3609 5181 3071 Size: 538 1572 3756 Fragments arranged by size: 3756 1572 538 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 2258 3175 3401 3553 5662 1493 Size: 765 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 765 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3187 4642 618 Size: 325 217 627 1400 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 217 BanII G_rGCy'C Cuts at: 1391 2047 2357 2364 3185 1391 Size: 656 310 7 821 4072 Fragments arranged by size: 4072 821 656 310 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BfrBI ATG'CAT Cuts at: 2414 2414 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4477 136 Size: 107 122 71 631 605 2805 1525 Fragments arranged by size: 2805 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 2600 3283 2600 Size: 683 5183 Bme1580I G_kGCm'C Cuts at: 1790 3927 5083 1790 Size: 2137 1156 2573 Fragments arranged by size: 2573 2137 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2359 2601 3118 Size: 416 53 83 399 24 942 242 517 Cuts at: 3118 3597 4555 442 Size: 479 958 1753 Fragments arranged by size: 1753 958 942 517 479 416 399 242 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2330 2330 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 4526 1239 Size: 1109 2178 2579 Fragments arranged by size: 2579 2178 1109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2420 3991 4856 5154 2420 Size: 1571 865 298 3132 Fragments arranged by size: 3132 1571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4529 882 Size: 617 3030 2219 Fragments arranged by size: 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 2057 5860 2057 Size: 3803 2063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2462 4299 4697 4971 5118 5529 2462 Size: 1837 398 274 147 411 2799 Fragments arranged by size: 2799 1837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5866 BseYI C'CCAG_C Cuts at: 2092 5069 5565 2092 Size: 2977 496 2393 Fragments arranged by size: 2977 2393 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2364 3927 4012 5083 2126 Size: 238 1563 85 1071 2909 Fragments arranged by size: 2909 1563 1071 238 85 BsmI GAATG_Cn' Cuts at: 2345 2747 3377 3470 2345 Size: 402 630 93 4741 Fragments arranged by size: 4741 630 402 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2126 2357 2364 3185 3927 4012 Size: 399 257 79 231 7 821 742 85 Cuts at: 4012 5083 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 821 742 399 257 231 85 79 7 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 2964 3755 5485 2964 Size: 791 1730 3345 Fragments arranged by size: 3345 1730 791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4510 4697 1700 Size: 110 2700 187 2869 Fragments arranged by size: 2869 2700 187 110 BsrGI T'GTAC_A Cuts at: 96 2226 2815 96 Size: 2130 589 3147 Fragments arranged by size: 3147 2130 589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5866 BstXI CCAn_nnnn'nTGG Cuts at: 2100 2100 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2058 3196 3563 3948 3965 5417 5503 5515 Size: 86 1138 367 385 17 1452 86 12 Cuts at: 5515 5861 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2742 2742 Size: 5866 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3382 4176 4196 2656 Size: 726 794 20 4326 Fragments arranged by size: 4326 794 726 20 ClaI AT'CG_AT Cuts at: 1554 2565 1554 Size: 1011 4855 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2559 2594 529 Size: 35 1995 35 3801 Fragments arranged by size: 3801 1995 35 35 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3046 3313 4202 Size: 54 1425 50 275 18 1216 267 889 Cuts at: 4202 5831 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1216 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2073 3796 1353 Size: 720 1723 3423 Fragments arranged by size: 3423 1723 720 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5866 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2348 2966 3929 4526 5313 5581 Size: 393 665 51 618 963 597 787 268 Cuts at: 5581 1239 Size: 1524 Fragments arranged by size: 1524 963 787 665 618 597 393 268 51 EcoRI G'AATT_C Cuts at: 2258 3175 3553 2258 Size: 917 378 4571 Fragments arranged by size: 4571 917 378 EcoRV GAT'ATC Cuts at: 3078 3078 Size: 5866 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2620 3007 2620 Size: 387 5479 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 3037 5013 1678 Size: 1359 1976 2531 Fragments arranged by size: 2531 1976 1359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 4507 4539 4581 Size: 32 135 32 494 32 2409 32 42 Cuts at: 4581 4613 1373 Size: 32 2626 Fragments arranged by size: 2626 2409 494 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2959 3210 3456 669 Size: 988 281 1021 251 246 3079 Fragments arranged by size: 3079 1021 988 281 251 246 HindIII A'AGCT_T Cuts at: 748 2602 748 Size: 1854 4012 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4980 5164 1305 Size: 3675 184 2007 Fragments arranged by size: 3675 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 2419 2792 3824 4183 4342 518 Size: 648 1087 166 373 1032 359 159 2042 Fragments arranged by size: 2042 1087 1032 648 373 359 166 159 MspA1I CmG'CkG Cuts at: 1606 2096 3282 3959 5107 5352 1606 Size: 490 1186 677 1148 245 2120 Fragments arranged by size: 2120 1186 1148 677 490 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NsiI A_TGCA'T Cuts at: 2416 2416 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2414 3224 4769 1132 Size: 97 1185 810 1545 2229 Fragments arranged by size: 2229 1545 1185 810 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2115 1613 Size: 502 5364 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2096 2096 Size: 5866 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 3214 4349 5030 5221 826 Size: 219 2169 1135 681 191 1471 Fragments arranged by size: 2169 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2435 3139 3970 4871 5133 820 Size: 197 926 492 704 831 901 262 1553 Fragments arranged by size: 1553 926 901 831 704 492 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 2901 3790 5 Size: 47 2849 889 2081 Fragments arranged by size: 2849 2081 889 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2004 3305 513 Size: 552 939 1301 3074 Fragments arranged by size: 3074 1301 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3893 3910 4063 4248 4693 325 Size: 1255 2313 17 153 185 445 1498 Fragments arranged by size: 2313 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2815 4112 Size: 274 80 33 51 1443 249 589 1297 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1297 589 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2602 2634 5826 5858 2602 Size: 32 3192 32 2610 Fragments arranged by size: 3192 2610 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3088 3202 3088 Size: 114 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2636 2636 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 2922 3995 2922 Size: 1073 4793 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI