Restriction map for
pFN21AB5237
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI NarI
NcoI NdeI NdeII NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 193 GTCTAC GT^[AC][GT]AC
AccII 29 CGCG CG^CG
AcsI 742 GAATTC [GA]^AATT[CT]
AgeI 352 ACCGGT A^CCGGT
ApoI 742 GAATTC [GA]^AATT[CT]
AspHI 348 GAGCAC G[AT]GC[AT]^C
AvaII 24 GGTCC G^G[AT]CC
BbvI 118 GCAGC GCAGC(8/12)
BglI 79 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 348 GAGCAC G[AGT]GC[ACT]^C
BpmI 406 CTGGAG CTGGAG(16/14)
BsiHKAI 348 GAGCAC G[AT]GC[AT]^C
BsmFI 25 GTCCC GGGAC(10/14)
Bsp1286I 348 GAGCAC G[AGT]GC[ACT]^C
BstUI 29 CGCG CG^CG
Cac8I 88 GCCGGC GC[ACGT]^[ACGT]GC
DraII 23 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 258 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 86 TGGCCG [CT]^GGCC[GA]
EarI 696 GAAGAG CTCTTC(1/4)
EcoO109I 23 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 742 GAATTC G^AATTC
FauI 27 CCCGC CCCGC(4/6)
Fnu4HI 118 GCAGC GC^[ACGT]GC
HaeII 91 GGCGCT [GA]GCGC^[CT]
HgaI 54 GACGC GACGC(5/10)
HincII 688 GTTAAC GT[CT]^[GA]AC
HindII 688 GTTAAC GT[CT]^[GA]AC
HinfI 672 GATTC G^A[ACGT]TC
HpaI 688 GTTAAC GTT^AAC
HphI 272 TCACC GGTGA(8/7)
ItaI 118 GCAGC GC^[ACGT]GC
Ksp632I 696 GAAGAG CTCTTC(1/4)
MaeII 304 ACGT A^CGT
MvnI 29 CGCG CG^CG
NaeI 88 GCCGGC GCC^GGC
NgoMIV 88 GCCGGC G^CCGGC
PinAI 352 ACCGGT A^CCGGT
PpuMI 23 GGGTCCC [GA]G^G[AT]CC[CT]
SmaI 20 CCCGGG CCC^GGG
SspI 753 AATATT AAT^ATT
TfiI 672 GATTC G^A[AT]TC
TseI 118 GCAGC G^C[AT]GC
XmaCI 20 CCCGGG C^CCGGG
XmaI 20 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 28 CCGC CCGC(-3/-1)
41 CCGC
AfaI 306 GTAC GT^AC
701 GTAC
AluI 771 AGCT AG^CT
853 AGCT
AvaI 20 CCCGGG C^[CT]CG[GA]G
34 CCCGAG
BbsI 73 GTCTTC GAAGAC(2/6)
319 GTCTTC
BpuAI 73 GTCTTC GAAGAC(2/6)
319 GTCTTC
BsaI 4 GAGACC GGTCTC(1/5)
37 GAGACC
BsaWI 352 ACCGGT [AT]^CCGG[AT]
692 ACCGGA
BsoBI 20 CCCGGG C^[CT]CG[GA]G
34 CCCGAG
BsrFI 88 GCCGGC [GA]^CCGG[CT]
352 ACCGGT
CfoI 30 GCGC GCG^C
92 GCGC
Cfr10I 88 GCCGGC [GA]^CCGG[CT]
352 ACCGGT
DdeI 215 CTAAG C^T[ACGT]AG
517 CTAAG
HhaI 30 GCGC GCG^C
92 GCGC
HinP1I 30 GCGC G^CGC
92 GCGC
MaeIII 266 GTGAC ^GT[ACGT]AC
469 GTGAC
MwoI 79 GCCCAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
92 GCGCTTCAGGC
PshAI 39 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
489 GACTTTAGTC
RsaI 306 GTAC GT^AC
701 GTAC
Tsp45I 266 GTGAC ^GT[GC]AC
469 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
BcnI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstNI Cfr13I EcoRII
FokI HaeIII HapII HpaII MboII
MnlI MseI MspI MvaI NciI
NlaIV Sau96I ScrFI Tru9I Tsp509I
TspRI