(Circular) (Six-base) MAPSORT of: pFN21AB5237.seq Check: 8314 from: 1 to: 5590 pFN21AB5237 5590 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2199 2933 1937 Size: 262 734 4594 Fragments arranged by size: 4594 734 262 Acc65I G'GTAC_C Cuts at: 2911 2911 Size: 5590 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2085 3653 5037 5305 1632 Size: 453 1568 1384 268 1917 Fragments arranged by size: 1917 1568 1384 453 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 2357 4421 2357 Size: 2064 3526 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5590 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2747 2899 3125 3277 5386 1493 Size: 1254 152 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 226 152 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2039 2915 1870 Size: 73 82 14 876 4545 Fragments arranged by size: 4545 876 82 73 14 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2911 4366 618 Size: 325 217 627 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 627 325 217 BanII G_rGCy'C Cuts at: 1391 2909 1391 Size: 1518 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 2317 928 Size: 971 172 246 4201 Fragments arranged by size: 4201 971 246 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3482 4692 3482 Size: 1210 4380 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 4201 136 Size: 107 122 71 631 605 418 2111 1525 Fragments arranged by size: 2111 1525 631 605 418 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 3007 3007 Size: 5590 Bme1580I G_kGCm'C Cuts at: 1790 3651 4807 1790 Size: 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5590 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 4250 1239 Size: 1193 1818 2579 Fragments arranged by size: 2579 1818 1193 Bpu10I CC'TnA_GC Cuts at: 2522 2522 Size: 5590 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2036 4253 882 Size: 617 504 33 2217 2219 Fragments arranged by size: 2219 2217 617 504 33 BsaAI yAC'GTr Cuts at: 493 1204 3381 493 Size: 711 2177 2702 Fragments arranged by size: 2702 2177 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3397 3779 4414 275 Size: 53 83 186 936 1864 382 635 1451 Fragments arranged by size: 1864 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2357 2697 4023 4421 4695 4842 5253 2357 Size: 340 1326 398 274 147 411 2694 Fragments arranged by size: 2694 1326 411 398 340 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 2766 2796 1178 Size: 30 165 30 960 30 373 30 3972 Fragments arranged by size: 3972 960 373 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5590 BseYI C'CCAG_C Cuts at: 2073 2366 4793 5289 2073 Size: 293 2427 496 2374 Fragments arranged by size: 2427 2374 496 293 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5017 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 3801 4829 1540 Size: 275 186 1039 761 1028 2301 Fragments arranged by size: 2301 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 3651 3736 4807 2357 Size: 1294 85 1071 3140 Fragments arranged by size: 3140 1294 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 2909 3651 3736 4807 1391 Size: 399 567 552 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 552 399 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2945 844 Size: 2101 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3477 1394 Size: 429 1654 3507 Fragments arranged by size: 3507 1654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4085 4259 65 Size: 1183 2837 174 1396 Fragments arranged by size: 2837 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2093 2357 4234 4421 1700 Size: 110 283 264 1877 187 2869 Fragments arranged by size: 2869 1877 283 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5590 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2679 3106 3900 3920 2679 Size: 427 794 20 4349 Fragments arranged by size: 4349 794 427 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2100 2135 529 Size: 35 1536 35 3984 Fragments arranged by size: 3984 1536 35 35 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DraIII CAC_nnn'GTG Cuts at: 2268 2268 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 3037 3926 Size: 54 1425 50 275 18 261 946 889 Cuts at: 3926 5555 8 Size: 1629 43 Fragments arranged by size: 1629 1425 946 889 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2696 3520 1353 Size: 1343 824 3423 Fragments arranged by size: 3423 1343 824 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4177 4551 4697 387 Size: 1064 138 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2432 3653 4250 5037 5305 1239 Size: 393 453 347 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 597 453 393 347 268 EcoO109I rG'GnC_Cy Cuts at: 2029 2029 Size: 5590 EcoRI G'AATT_C Cuts at: 2747 2899 3277 2747 Size: 152 378 5060 Fragments arranged by size: 5060 378 152 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2100 4737 1678 Size: 422 2637 2531 Fragments arranged by size: 2637 2531 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2695 2934 3180 669 Size: 988 281 757 239 246 3079 Fragments arranged by size: 3079 988 757 281 246 239 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 2695 3180 2695 Size: 485 5105 KpnI G_GTAC'C Cuts at: 2915 2915 Size: 5590 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3768 Fragments arranged by size: 3768 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3548 3907 4066 518 Size: 648 2382 359 159 2042 Fragments arranged by size: 2382 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3006 3683 4831 5076 1606 Size: 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 677 245 NaeI GCC'GGC Cuts at: 2095 2095 Size: 5590 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NgoMIV G'CCGG_C Cuts at: 2093 2093 Size: 5590 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2948 4493 1132 Size: 97 1719 1545 2229 Fragments arranged by size: 2229 1719 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5590 PfoI T'CCnGG_A Cuts at: 2529 2529 Size: 5590 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2344 2376 3659 3691 5172 5204 2344 Size: 32 1283 32 1481 32 2730 Fragments arranged by size: 2730 1481 1283 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2029 2029 Size: 5590 PshAI GACnn'nnGTC Cuts at: 1613 2048 2498 1613 Size: 435 450 4705 Fragments arranged by size: 4705 450 435 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PstI C_TGCA'G Cuts at: 830 2942 830 Size: 2112 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5590 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SanDI GG'GwC_CC Cuts at: 2029 2029 Size: 5590 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2938 4073 4754 4945 826 Size: 219 1893 1135 681 191 1471 Fragments arranged by size: 1893 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2027 2917 1872 Size: 155 890 4545 Fragments arranged by size: 4545 890 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 3694 4595 4857 820 Size: 197 926 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2948 1132 Size: 1816 3774 SspI AAT'ATT Cuts at: 5 52 2760 3514 5 Size: 47 2708 754 2081 Fragments arranged by size: 2708 2081 754 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 3029 513 Size: 552 939 1025 3074 Fragments arranged by size: 3074 1025 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3617 3634 3787 3972 4417 325 Size: 1255 2037 17 153 185 445 1498 Fragments arranged by size: 2037 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2705 3836 5548 Size: 274 80 33 51 1443 728 1131 1712 Cuts at: 5548 96 Size: 138 Fragments arranged by size: 1712 1443 1131 728 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2451 2483 5550 5582 2451 Size: 32 3067 32 2459 Fragments arranged by size: 3067 2459 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2025 2915 1870 Size: 155 890 4545 Fragments arranged by size: 4545 890 155 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI