Restriction map for
pFN21AE1538
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MamI
MboII MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp509I TthHB8I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AspI 97 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 199 GGTCC G^G[AT]CC
BfaI 58 CTAG C^TAG
BsaJI 294 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 242 TCCGGT [AT]^CCGG[AT]
BsmAI 205 GTCTC GTCTC(1/5)
BsmFI 46 GGGAC GGGAC(10/14)
BstF5I 329 GGATG GGATG(2/0)
BstYI 132 AGATCC [GA]^GATC[CT]
Cac8I 7 GCCAGC GC[ACGT]^[ACGT]GC
DdeI 212 CTAAG C^T[ACGT]AG
EcoNI 82 CCTGTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 326 GCGGG CCCGC(4/6)
FokI 329 GGATG GGATG(9/13)
HapII 243 CCGG C^CGG
HgaI 48 GACGC GACGC(5/10)
HpaII 243 CCGG C^CGG
MaeI 58 CTAG C^TAG
MaeIII 70 GTCAC ^GT[ACGT]AC
MflI 132 AGATCC [GA]^GATC[CT]
MspI 243 CCGG C^CGG
MwoI 311 GCTGTGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 252 CATG CATG^
NlaIV 220 GGCTCC GG[ACGT]^[ACGT]CC
PflFI 97 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 118 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 65 CAGCTG CAG^CTG
Tsp45I 70 GTCAC ^GT[GC]AC
TspRI 99 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 97 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 118 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 57 TCTAGA T^CTAGA
XhoII 132 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 270 CCGC CCGC(-3/-1)
326 GCGG
AluI 13 AGCT AG^CT
66 AGCT
AlwI 133 GATCC GGATC(4/5)
240 GATCC
BsrI 190 CCAGT ACTGG(1/-1)
202 CCAGT
BssKI 217 CCTGG ^CC[ACGT]GG
295 CCAGG
BstNI 217 CCTGG CC^[AT]GG
295 CCAGG
CfoI 181 GCGC GCG^C
248 GCGC
Cfr13I 5 GGGCC G^G[ACGT]CC
199 GGTCC
EcoRII 217 CCTGG ^CC[AT]GG
295 CCAGG
HhaI 181 GCGC GCG^C
248 GCGC
HinP1I 181 GCGC G^CGC
248 GCGC
HinfI 258 GAATC G^A[ACGT]TC
299 GAATC
MspA1I 65 CAGCTG C[AC]G^C[GT]G
270 CCGCTG
MvaI 217 CCTGG CC^[AT]GG
295 CCAGG
Sau96I 5 GGGCC G^G[ACGT]CC
199 GGTCC
ScrFI 217 CCTGG CC^[ACGT]GG
295 CCAGG
TfiI 258 GAATC G^A[AT]TC
299 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BsiYI BslI DpnII Fnu4HI
HaeIII ItaI MboI MnlI MseI
NdeII Sau3AI Tru9I TseI