(Linear) (Six-base) MAPSORT of: KIEE1538.seq  Check: 7420  from: 1  to: 351



KIEE1538.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:41  ..

BsaWI w'CCGG_w

Cuts at:      0     242     351 
   Size:        242     109
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      68      98     351 
   Size:         68      30     253

  Fragments arranged by size: 

                 253      68      30
 
BseYI C'CCAG_C

Cuts at:      0      43     351 
   Size:         43     308
 
BstYI r'GATC_y

Cuts at:      0     132     351 
   Size:        132     219
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      24     351 
   Size:         24     327
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      86     351 
   Size:         86     265
 
MspA1I CmG'CkG

Cuts at:      0      67     272     351 
   Size:         67     205      79

  Fragments arranged by size: 

                 205      79      67
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     124     351 
   Size:        124     227
 
PvuII CAG'CTG

Cuts at:      0      67     351 
   Size:         67     284
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      63      95     351 
   Size:         63      32     256

  Fragments arranged by size: 

                 256      63      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     100     351 
   Size:        100     251
 
XbaI T'CTAG_A

Cuts at:      0      57     351 
   Size:         57     294
 

 Enzymes that do cut:

    BsaWI    BsaXI    BseYI    BstYI    BtgZI    EcoNI   MspA1I    PflMI
    PvuII     TstI  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     XcmI     XhoI     XmaI     XmnI     ZraI