################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 103 GTCTAC GT^[AC][GT]AC AcsI 364 GAATTC [GA]^AATT[CT] AocI 373 CCTGAGG CC^T[ACGT]AGG ApoI 364 GAATTC [GA]^AATT[CT] AvaII 113 GGACC G^G[AT]CC BalI 413 TGGCCA TGG^CCA BamHI 297 GGATCC G^GATCC BanI 250 GGCACC G^G[CT][GA]CC BbvI 288 GCTGC GCAGC(8/12) BglI 41 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiYI 83 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 83 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrI 416 CCAGT ACTGG(1/-1) BstEII 333 GGTGACC G^GT[ACGT]ACC BstF5I 237 GGATG GGATG(2/0) BstPI 333 GGTGACC G^GT[ACGT]ACC BstYI 297 GGATCC [GA]^GATC[CT] Bsu36I 373 CCTGAGG CC^T[ACGT]AGG Cac8I 381 GCAGGC GC[ACGT]^[ACGT]GC Cfr13I 113 GGACC G^G[ACGT]CC DsaI 109 CCATGG C^C[GA][CT]GG EaeI 413 TGGCCA [CT]^GGCC[GA] Eco81I 373 CCTGAGG CC^T[ACGT]AGG EcoO65I 333 GGTGACC G^GT[ACGT]ACC EcoRI 364 GAATTC G^AATTC Fnu4HI 288 GCTGC GC^[ACGT]GC FokI 237 GGATG GGATG(9/13) HaeIII 414 GGCC GG^CC ItaI 288 GCTGC GC^[ACGT]GC MflI 297 GGATCC [GA]^GATC[CT] MluNI 413 TGGCCA TGG^CCA MscI 413 TGGCCA TGG^CCA MslI 232 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 262 CAGCTG C[AC]G^C[GT]G MwoI 41 GCCTGTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 109 CCATGG C^CATGG NspI 291 GCATGT [GA]CATG^[CT] PstI 407 CTGCAG CTGCA^G PvuII 262 CAGCTG CAG^CTG Sau96I 113 GGACC G^G[ACGT]CC SfcI 407 CTGCAG C^T[GA][CT]AG SmlI 244 CTCAAG C^T[CT][GA]AG TseI 288 GCTGC G^C[AT]GC Tsp509I 365 AATT ^AATT XcmI 224 CCACAAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 297 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AlwI 298 GATCC GGATC(4/5) 297 GGATC BmyI 249 GGGCAC G[AGT]GC[ACT]^C 419 GTGCCC BpmI 429 CTCCAG CTGGAG(16/14) 71 CTGGAG BseRI 16 GAGGAG GAGGAG(10/8) 74 GAGGAG BsgI 274 CTGCAC GTGCAG(16/14) 379 GTGCAG Bsp1286I 249 GGGCAC G[AGT]GC[ACT]^C 419 GTGCCC DpnII 241 GATC ^GATC 298 GATC EcoT14I 109 CCATGG C^C[AT][AT]GG 212 CCTTGG MboI 241 GATC ^GATC 298 GATC MboII 64 GAAGA GAAGA(8/7) 396 GAAGA NdeII 241 GATC ^GATC 298 GATC Sau3AI 241 GATC ^GATC 298 GATC SfaNI 67 GATGC GCATC(5/9) 220 GATGC StyI 109 CCATGG C^C[AT][AT]GG 212 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BsaJI BssKI BstNI DdeI EcoRII HphI MaeIII MnlI MvaI NlaIII NlaIV ScrFI Tsp45I TspRI