################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 15 GTAC GT^AC AluI 152 AGCT AG^CT BcnI 136 CCGGG CC^[GC]GG BsaJI 108 CCAGGG C^C[ACGT][ACGT]GG BseRI 70 GAGGAG GAGGAG(10/8) BsgI 64 CTGCAC GTGCAG(16/14) BssSI 67 CACGAG CACGAG(-5/-1) BstNI 108 CCAGG CC^[AT]GG BstXI 108 CCAGGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 27 GCAGGC GC[ACGT]^[ACGT]GC DpnII 170 GATC ^GATC EarI 130 GAAGAG CTCTTC(1/4) EcoRII 108 CCAGG ^CC[AT]GG HaeIII 3 GGCC GG^CC HapII 136 CCGG C^CGG HgaI 44 GCGTC GACGC(5/10) HincII 46 GTCAAC GT[CT]^[GA]AC HindII 46 GTCAAC GT[CT]^[GA]AC HinfI 77 GATTC G^A[ACGT]TC HpaII 136 CCGG C^CGG Ksp632I 130 GAAGAG CTCTTC(1/4) MaeIII 228 GTTAC ^GT[ACGT]AC MboI 170 GATC ^GATC MboII 130 GAAGA GAAGA(8/7) MseI 143 TTAA T^TAA MslI 54 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 136 CCGG C^CGG MvaI 108 CCAGG CC^[AT]GG MwoI 66 GCACGAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 136 CCGGG CC^[GC]GG NdeII 170 GATC ^GATC PstI 25 CTGCAG CTGCA^G RsaI 15 GTAC GT^AC SapI 130 GAAGAGC GCTCTTC(1/4) Sau3AI 170 GATC ^GATC SfcI 25 CTGCAG C^T[GA][CT]AG SmlI 82 CTCAAG C^T[CT][GA]AG TaqI 168 TCGA T^CGA TfiI 77 GATTC G^A[AT]TC Tru9I 143 TTAA T^TAA TthHB8I 168 TCGA T^CGA XcmI 108 CCAGGGAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### BssKI 108 CCAGG ^CC[ACGT]GG 136 CCGGG MfeI 215 CAATTG C^AATTG 221 CAATTG MnlI 81 CCTC CCTC(7/6) 70 GAGG MunI 215 CAATTG C^AATTG 221 CAATTG ScrFI 108 CCAGG CC^[ACGT]GG 136 CCGGG TspRI 20 CACTG CA[GC]TG[ACGT][ACGT]^ 59 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### BbvI Fnu4HI ItaI TseI Tsp509I