(Circular) (Six-base) MAPSORT of: pFN21AE4371.seq Check: 1626 from: 1 to: 5875 pFN21AE4371 5875 bp With 182 enzymes: SgfI * March 28, 2012 13:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2307 2307 Size: 5875 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 2582 3938 5322 5590 1632 Size: 504 446 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 504 446 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2186 2552 1184 Size: 1002 366 4507 Fragments arranged by size: 4507 1002 366 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2495 2527 2495 Size: 32 5843 AlwNI CAG_nnn'CTG Cuts at: 3104 3173 3618 5190 3104 Size: 69 445 1572 3789 Fragments arranged by size: 3789 1572 445 69 ApaI G_GGCC'C Cuts at: 2018 2203 2409 2018 Size: 185 206 5484 Fragments arranged by size: 5484 206 185 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2018 3200 1870 Size: 73 75 1182 4545 Fragments arranged by size: 4545 1182 75 73 BamHI G'GATC_C Cuts at: 2854 3205 3572 2854 Size: 351 367 5157 Fragments arranged by size: 5157 367 351 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2116 2266 2869 3196 4651 Size: 325 217 627 329 150 603 327 1455 Cuts at: 4651 618 Size: 1842 Fragments arranged by size: 1842 1455 627 603 329 327 325 217 150 BanII G_rGCy'C Cuts at: 1391 2018 2189 2203 2409 2627 2813 3194 1391 Size: 627 171 14 206 218 186 381 4072 Fragments arranged by size: 4072 627 381 218 206 186 171 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2224 928 Size: 971 325 4579 Fragments arranged by size: 4579 971 325 BbvCI CC'TCA_GC Cuts at: 3166 3166 Size: 5875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4066 4100 1903 Size: 34 50 34 2045 34 3678 Fragments arranged by size: 3678 2045 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2364 4486 Size: 107 122 71 631 605 388 304 2122 Cuts at: 4486 136 Size: 1525 Fragments arranged by size: 2122 1525 631 605 388 304 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2018 2121 2203 2271 2409 2703 3069 3936 Size: 228 103 82 68 138 294 366 867 Cuts at: 3936 5092 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 867 366 294 228 138 103 82 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2110 3181 2110 Size: 1071 4804 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 2702 3023 4535 1239 Size: 1379 84 321 1512 2579 Fragments arranged by size: 2579 1512 1379 321 84 Bpu10I CC'TnA_GC Cuts at: 2639 3166 2639 Size: 527 5348 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4000 4865 5163 4000 Size: 865 298 4712 Fragments arranged by size: 4712 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2985 4538 882 Size: 617 1486 1553 2219 Fragments arranged by size: 2219 1553 1486 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4308 4706 4980 5127 5538 4308 Size: 398 274 147 411 4645 Fragments arranged by size: 4645 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5650 Fragments arranged by size: 5650 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 2768 3094 1186 Size: 1105 477 326 3967 Fragments arranged by size: 3967 1105 477 326 BseYI C'CCAG_C Cuts at: 2202 2370 3015 5078 5574 2202 Size: 168 645 2063 496 2503 Fragments arranged by size: 2503 2063 645 496 168 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2009 1163 Size: 573 273 5029 Fragments arranged by size: 5029 573 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2368 3325 4086 5114 1540 Size: 275 186 367 957 761 1028 2301 Fragments arranged by size: 2301 1028 957 761 367 275 186 BsiHKAI G_wGCw'C Cuts at: 2813 3936 4021 5092 2813 Size: 1123 85 1071 3596 Fragments arranged by size: 3596 1123 1071 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2121 2189 2203 2271 2409 2627 Size: 399 228 103 68 14 68 138 218 Cuts at: 2627 2703 2813 3069 3194 3936 4021 5092 1391 Size: 76 110 256 125 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 256 228 218 138 125 110 103 85 76 68 68 14 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2685 3230 844 Size: 1463 378 545 3489 Fragments arranged by size: 3489 1463 545 378 BsrBI CCG'CTC Cuts at: 1394 1823 2589 2748 3762 1394 Size: 429 766 159 1014 3507 Fragments arranged by size: 3507 1014 766 429 159 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2358 2478 2556 2823 4519 4706 1700 Size: 110 548 120 78 267 1696 187 2869 Fragments arranged by size: 2869 1696 548 267 187 120 110 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2710 2710 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2547 2565 2854 3205 3572 3957 3974 5426 Size: 575 18 289 351 367 385 17 1452 Cuts at: 5426 5512 5524 5870 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 575 385 367 351 346 289 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2010 2112 2925 3024 2010 Size: 102 813 99 4861 Fragments arranged by size: 4861 813 102 99 BtgI C'CryG_G Cuts at: 513 1065 2158 2401 513 Size: 552 1093 243 3987 Fragments arranged by size: 3987 1093 552 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2137 3109 3391 4185 4205 2137 Size: 972 282 794 20 3807 Fragments arranged by size: 3807 972 794 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DraIII CAC_nnn'GTG Cuts at: 2698 2698 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2365 2830 Size: 54 1425 50 275 18 201 334 465 Cuts at: 2830 3106 3322 4211 5840 8 Size: 276 216 889 1629 43 Fragments arranged by size: 1629 1425 889 465 334 276 275 216 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2365 3322 1537 Size: 275 553 957 4090 Fragments arranged by size: 4090 957 553 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3805 1353 Size: 2452 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2345 4462 4836 4982 387 Size: 1064 138 756 2117 374 146 1280 Fragments arranged by size: 2117 1280 1064 756 374 146 138 EcoICRI GAG'CTC Cuts at: 2811 2811 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2582 2618 2702 3023 3938 4535 Size: 393 504 446 36 84 321 915 597 Cuts at: 4535 5322 5590 1239 Size: 787 268 1524 Fragments arranged by size: 1524 915 787 597 504 446 393 321 268 84 36 EcoNI CCTnn'n_nnAGG Cuts at: 3041 3041 Size: 5875 EcoO109I rG'GnC_Cy Cuts at: 2014 2015 2199 2374 2406 2494 2602 2962 3046 Size: 1 184 175 32 88 108 360 84 Cuts at: 3046 3162 2014 Size: 116 4727 Fragments arranged by size: 4727 360 184 175 116 108 88 84 32 1 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2066 5022 1678 Size: 388 2956 2531 Fragments arranged by size: 2956 2531 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 4516 4548 4590 Size: 32 135 32 1556 32 1356 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 1556 1356 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4989 5173 1305 Size: 3684 184 2007 Fragments arranged by size: 3684 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 3108 10 Size: 54 1425 343 201 1075 2777 Fragments arranged by size: 2777 1425 1075 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 3833 4192 4351 518 Size: 648 2667 359 159 2042 Fragments arranged by size: 2667 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 2263 2370 2403 2917 3019 3291 3968 Size: 554 103 107 33 514 102 272 677 Cuts at: 3968 5116 5361 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 554 514 272 245 107 103 102 33 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5323 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2248 2800 3233 4778 1132 Size: 97 1019 552 433 1545 2229 Fragments arranged by size: 2229 1545 1019 552 433 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2499 2595 2857 2969 2499 Size: 96 262 112 5405 Fragments arranged by size: 5405 262 112 96 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 3046 3162 2374 Size: 672 116 5087 Fragments arranged by size: 5087 672 116 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2014 2199 2405 2014 Size: 185 206 5484 Fragments arranged by size: 5484 206 185 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2417 2449 2417 Size: 32 5843 PstI C_TGCA'G Cuts at: 830 2060 2610 2667 2820 3116 3227 830 Size: 1230 550 57 153 296 111 3478 Fragments arranged by size: 3478 1230 550 296 153 111 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2263 2917 2263 Size: 654 5221 SacI G_AGCT'C Cuts at: 2813 2813 Size: 5875 SacII CC_GC'GG Cuts at: 2161 2404 2161 Size: 243 5632 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 2060 2820 3227 2060 Size: 760 407 4708 Fragments arranged by size: 4708 760 407 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2056 2606 2663 2816 3112 3223 4358 Size: 219 1011 550 57 153 296 111 1135 Cuts at: 4358 5039 5230 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1011 681 550 296 219 191 153 111 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3202 1872 Size: 1330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3979 4880 5142 820 Size: 197 926 2036 901 262 1553 Fragments arranged by size: 2036 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 2800 3233 1132 Size: 1668 433 3774 Fragments arranged by size: 3774 1668 433 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2054 2471 1517 Size: 537 417 4921 Fragments arranged by size: 4921 537 417 StyI C'CwwG_G Cuts at: 513 1065 2377 2703 2904 3147 3314 513 Size: 552 1312 326 201 243 167 3074 Fragments arranged by size: 3074 1312 552 326 243 201 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2581 2887 3902 3919 4072 4257 4702 Size: 1255 1001 306 1015 17 153 185 445 Cuts at: 4702 325 Size: 1498 Fragments arranged by size: 1498 1255 1015 1001 445 306 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4121 5833 96 Size: 274 80 33 51 1443 2144 1712 138 Fragments arranged by size: 2144 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2855 2855 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3200 1870 Size: 1330 4545 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI