################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AlwI AlwNI AocI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbvCI BciVI BclI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraII DraIII DrdI Eam1105I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### AccII 37 CGCG CG^CG AfaI 325 GTAC GT^AC AflII 159 CTTAAG C^TTAAG AflIII 549 ACGTGT A^C[GA][CT]GT Aor51HI 70 AGCGCT AGC^GCT ApaI 866 GGGCCC GGGCC^C AseI 529 ATTAAT AT^TAAT AsnI 529 ATTAAT AT^TAAT BalI 308 TGGCCA TGG^CCA BanII 866 GGGCCC G[GA]GC[CT]^C BbrPI 548 CACGTG CAC^GTG BbsI 412 GTCTTC GAAGAC(2/6) BcgI 147 CGATAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 57 CCGGG CC^[GC]GG BfrI 159 CTTAAG C^TTAAG BglII 395 AGATCT A^GATCT BpuAI 412 GTCTTC GAAGAC(2/6) BsaAI 548 CACGTG [CT]AC^GT[GA] BsgI 734 GTGCAG GTGCAG(16/14) BsiEI 27 CGGCCG CG[GA][CT]^CG BspDI 145 ATCGAT AT^CGAT BstF5I 669 GGATG GGATG(2/0) BstUI 37 CGCG CG^CG BstYI 395 AGATCT [GA]^GATC[CT] ClaI 145 ATCGAT AT^CGAT DdeI 632 CTAAG C^T[ACGT]AG DpnII 396 GATC ^GATC DraI 724 TTTAAA TTT^AAA EagI 27 CGGCCG C^GGCCG EclXI 27 CGGCCG C^GGCCG Eco47III 70 AGCGCT AGC^GCT Eco52I 27 CGGCCG C^GGCCG EcoNI 689 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 767 GAATTC G^AATTC FauI 38 GCGGG CCCGC(4/6) FokI 669 GGATG GGATG(9/13) HapII 57 CCGG C^CGG HpaII 57 CCGG C^CGG HphI 334 GGTGA GGTGA(8/7) KspI 36 CCGCGG CCGC^GG MaeII 549 ACGT A^CGT MaeIII 857 GTGAC ^GT[ACGT]AC MboI 396 GATC ^GATC MflI 395 AGATCT [GA]^GATC[CT] MluNI 308 TGGCCA TGG^CCA MscI 308 TGGCCA TGG^CCA MslI 300 CACGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 57 CCGG C^CGG MvnI 37 CGCG CG^CG NciI 57 CCGGG CC^[GC]GG NcoI 875 CCATGG C^CATGG NdeII 396 GATC ^GATC PmaCI 548 CACGTG CAC^GTG PmlI 548 CACGTG CAC^GTG PshBI 529 ATTAAT AT^TAAT PstI 516 CTGCAG CTGCA^G PvuII 538 CAGCTG CAG^CTG RsaI 325 GTAC GT^AC SacII 36 CCGCGG CCGC^GG SapI 66 GAAGAGC GCTCTTC(1/4) Sau3AI 396 GATC ^GATC ScaI 324 AGTACT AGT^ACT SmlI 159 CTTAAG C^T[CT][GA]AG SspI 787 AATATT AAT^ATT SwaI 723 ATTTAAAT ATTT^AAAT Tsp45I 857 GTGAC ^GT[GC]AC XhoII 395 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Asp700I 579 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 922 GAAGGTATTC AvaII 190 GGACC G^G[AT]CC 555 GGACC BfaI 268 CTAG C^TAG 676 CTAG BmyI 297 GGGCAC G[AGT]GC[ACT]^C 866 GGGCCC BpmI 74 CTGGAG CTGGAG(16/14) 500 CTGGAG Bsp1286I 297 GGGCAC G[AGT]GC[ACT]^C 866 GGGCCC BstNI 193 CCTGG CC^[AT]GG 499 CCTGG CfoI 54 GCGC GCG^C 71 GCGC DsaI 36 CCGCGG C^C[GA][CT]GG 875 CCATGG EaeI 27 CGGCCG [CT]^GGCC[GA] 308 TGGCCA EarI 399 CTCTTC CTCTTC(1/4) 66 GAAGAG EcoRII 193 CCTGG ^CC[AT]GG 499 CCTGG HaeII 53 AGCGCC [GA]GCGC^[CT] 70 AGCGCT HhaI 54 GCGC GCG^C 71 GCGC HinP1I 54 GCGC G^CGC 71 GCGC Ksp632I 399 CTCTTC CTCTTC(1/4) 66 GAAGAG MaeI 268 CTAG C^TAG 676 CTAG MspA1I 36 CCGCGG C[AC]G^C[GT]G 538 CAGCTG MvaI 193 CCTGG CC^[AT]GG 499 CCTGG NlaIV 19 GGGGCC GG[ACGT]^[ACGT]CC 866 GGGCCC PleI 80 GACTC GAGTC(4/5) 521 GACTC SfcI 516 CTGCAG C^T[GA][CT]AG 762 CTACAG TaqI 146 TCGA T^CGA 432 TCGA TthHB8I 146 TCGA T^CGA 432 TCGA XmnI 579 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 922 GAAGGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BsaJI BsiYI BslI BsrI BssKI Cac8I Cfr13I EcoT14I Fnu4HI HaeIII HinfI ItaI MboII MnlI MseI MwoI NlaIII Sau96I ScrFI StyI TfiI Tru9I TseI Tsp509I TspRI