(Circular) (Six-base) MAPSORT of: pFN21AE1539.seq Check: 7996 from: 1 to: 5644 pFN21AE1539 5644 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2965 2965 Size: 5644 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 2575 3707 5091 5359 1632 Size: 458 485 1132 1384 268 1917 Fragments arranged by size: 1917 1384 1132 485 458 268 AfeI AGC'GCT Cuts at: 2077 2077 Size: 5644 AflII C'TTAA_G Cuts at: 820 1017 2164 820 Size: 197 1147 4300 Fragments arranged by size: 4300 1147 197 AflIII A'CryG_T Cuts at: 1203 2554 4543 5520 1203 Size: 1351 1989 977 1327 Fragments arranged by size: 1989 1351 1327 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2309 1166 Size: 1143 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5644 AlwNI CAG_nnn'CTG Cuts at: 3387 4959 3387 Size: 1572 4072 ApaI G_GGCC'C Cuts at: 2875 2875 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2386 2477 2772 2953 3179 3331 5440 1493 Size: 893 91 295 181 226 152 2109 1697 Fragments arranged by size: 2109 1697 893 295 226 181 152 91 AseI AT'TA_AT Cuts at: 160 1032 2535 4198 160 Size: 872 1503 1663 1606 Fragments arranged by size: 1663 1606 1503 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2969 1870 Size: 73 1026 4545 Fragments arranged by size: 4545 1026 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2364 2397 2364 Size: 33 5611 BamHI G'GATC_C Cuts at: 2974 3341 2974 Size: 367 5277 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2965 4420 618 Size: 325 217 627 1178 1455 1842 Fragments arranged by size: 1842 1455 1178 627 325 217 BanII G_rGCy'C Cuts at: 1391 2875 2963 1391 Size: 1484 88 4072 Fragments arranged by size: 4072 1484 88 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2410 928 Size: 971 511 4162 Fragments arranged by size: 4162 971 511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2141 2175 3835 3869 1903 Size: 34 50 34 120 34 1660 34 3678 Fragments arranged by size: 3678 1660 120 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3536 4746 3536 Size: 1210 4434 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4255 136 Size: 107 122 71 631 605 2583 1525 Fragments arranged by size: 2583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2400 5639 2400 Size: 3239 2405 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2306 2875 3705 4861 1790 Size: 516 569 830 1156 2573 Fragments arranged by size: 2573 1156 830 569 516 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3375 4333 442 Size: 416 53 83 399 24 1958 958 1753 Fragments arranged by size: 1958 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2526 4304 1239 Size: 861 426 1778 2579 Fragments arranged by size: 2579 1778 861 426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4307 882 Size: 617 2808 2219 Fragments arranged by size: 2808 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2555 3435 493 Size: 711 1351 880 2702 Fragments arranged by size: 2702 1351 880 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5419 Fragments arranged by size: 5419 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5644 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2760 1163 Size: 573 1024 4047 Fragments arranged by size: 4047 1024 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2035 3094 3855 4883 1540 Size: 275 186 34 1059 761 1028 2301 Fragments arranged by size: 2301 1059 1028 761 275 186 34 BsiHKAI G_wGCw'C Cuts at: 3705 3790 4861 3705 Size: 85 1071 4488 Fragments arranged by size: 4488 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2306 2875 2963 3705 3790 4861 1391 Size: 399 516 569 88 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 516 399 88 85 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2999 844 Size: 2155 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4288 4475 1700 Size: 110 2478 187 2869 Fragments arranged by size: 2869 2478 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstYI r'GATC_y Cuts at: 1972 2400 2974 3341 3726 3743 5195 5281 5293 Size: 428 574 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 574 428 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2041 2880 513 Size: 552 976 839 3277 Fragments arranged by size: 3277 976 839 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 2748 3160 3954 3974 2748 Size: 412 794 20 4418 Fragments arranged by size: 4418 794 412 20 ClaI AT'CG_AT Cuts at: 1554 2151 1554 Size: 597 5047 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2731 2948 2731 Size: 217 5427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2032 2313 3091 Size: 54 1425 50 275 18 202 281 778 Cuts at: 3091 3980 5609 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 778 281 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2032 3091 1537 Size: 275 220 1059 4090 Fragments arranged by size: 4090 1059 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2066 2410 3574 1353 Size: 713 344 1164 3423 Fragments arranged by size: 3423 1164 713 344 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2100 2526 2575 3707 4304 5091 Size: 393 458 10 426 49 1132 597 787 Cuts at: 5091 5359 1239 Size: 268 1524 Fragments arranged by size: 1524 1132 787 597 458 426 393 268 49 10 EcoNI CCTnn'n_nnAGG Cuts at: 2698 2698 Size: 5644 EcoRI G'AATT_C Cuts at: 2772 2953 3331 2772 Size: 181 378 5085 Fragments arranged by size: 5085 378 181 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2605 2179 Size: 426 5218 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 2062 2079 4791 1678 Size: 384 17 2712 2531 Fragments arranged by size: 2712 2531 384 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4285 4317 4359 4391 1373 Size: 32 135 32 2713 32 42 32 2626 Fragments arranged by size: 2713 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2988 3234 669 Size: 988 281 1050 246 3079 Fragments arranged by size: 3079 1050 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5644 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2969 2969 Size: 5644 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2173 2512 4758 4942 1305 Size: 868 339 2246 184 2007 Fragments arranged by size: 2246 2007 868 339 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2315 10 Size: 54 1425 343 483 3339 Fragments arranged by size: 3339 1425 483 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 3602 3961 4120 518 Size: 648 1143 1293 359 159 2042 Fragments arranged by size: 2042 1293 1143 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2043 2545 3060 3737 4885 5130 1606 Size: 437 502 515 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 515 502 437 245 NcoI C'CATG_G Cuts at: 513 1065 2880 513 Size: 552 1815 3277 Fragments arranged by size: 3277 1815 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 3002 4547 1132 Size: 97 1773 1545 2229 Fragments arranged by size: 2229 1773 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 4543 4543 Size: 5644 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PmlI CAC'GTG Cuts at: 2555 2555 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3745 5226 5258 3713 Size: 32 1481 32 4099 Fragments arranged by size: 4099 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 2798 3214 2798 Size: 416 5228 PspOMI G'GGCC_C Cuts at: 2871 2871 Size: 5644 PstI C_TGCA'G Cuts at: 830 2525 2996 830 Size: 1695 471 3478 Fragments arranged by size: 3478 1695 471 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2545 2545 Size: 5644 SacII CC_GC'GG Cuts at: 2044 2044 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SapI GCTCTTCn'nnn_ Cuts at: 2066 2066 Size: 5644 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 2331 3892 5604 2331 Size: 1561 1712 2371 Fragments arranged by size: 2371 1712 1561 SfcI C'TryA_G Cuts at: 826 1045 2521 2767 2992 4127 4808 4999 826 Size: 219 1476 246 225 1135 681 191 1471 Fragments arranged by size: 1476 1471 1135 681 246 225 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2971 1872 Size: 1099 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2164 3748 4649 4911 820 Size: 197 926 221 1584 901 262 1553 Fragments arranged by size: 1584 1553 926 901 262 221 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 3002 1132 Size: 1870 3774 SspI AAT'ATT Cuts at: 5 52 2794 3568 5 Size: 47 2742 774 2081 Fragments arranged by size: 2742 2081 774 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5644 StyI C'CwwG_G Cuts at: 513 1065 2267 2604 2880 3083 513 Size: 552 1202 337 276 203 3074 Fragments arranged by size: 3074 1202 552 337 276 203 SwaI ATTT'AAAT Cuts at: 2731 2731 Size: 5644 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2327 2668 3671 3688 3841 4026 4471 Size: 1255 747 341 1003 17 153 185 445 Cuts at: 4471 325 Size: 1498 Fragments arranged by size: 1498 1255 1003 747 445 341 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2329 3890 5602 Size: 274 80 33 51 1443 352 1561 1712 Cuts at: 5602 96 Size: 138 Fragments arranged by size: 1712 1561 1443 352 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5644 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2969 1870 Size: 1099 4545 XmnI GAAnn'nnTTC Cuts at: 2588 2931 3773 2588 Size: 343 842 4459 Fragments arranged by size: 4459 842 343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI XcmI