Restriction map for
pFN21AB8012
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspI BalI
BamHI BbeI BbsI BciVI BfrI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
Eco47III EcoO65I EcoRV EcoT22I FseI
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 1351 CGCG CG^CG
AcyI 1360 GGCGTC G[GA]^CG[CT]C
AspHI 361 GAGCTC G[AT]GC[AT]^C
AviII 149 TGCGCA TGC^GCA
AvrII 1122 CCTAGG C^CTAGG
BbrPI 1432 CACGTG CAC^GTG
BbvCI 1035 CCTCAGC CCTCAGC(-5/-2)
BcgI 1911 CGAGCCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 410 TGATCA T^GATCA
BglII 1484 AGATCT A^GATCT
BlnI 1122 CCTAGG C^CTAGG
BsaHI 1360 GGCGTC G[GA]^CG[CT]C
BsiHKAI 361 GAGCTC G[AT]GC[AT]^C
BsmBI 748 GAGACG CGTCTC(1/5)
BsmI 1858 GCATTC GAATGC(1/-1)
BspLU11I 1898 ACATGT A^CATGT
BsrDI 266 GCAATG GCAATG(2/0)
BssSI 1400 CTCGTG CACGAG(-5/-1)
BstAPI 2017 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1351 CGCG CG^CG
EarI 499 GAAGAG CTCTTC(1/4)
EcoRI 1585 GAATTC G^AATTC
FbaI 410 TGATCA T^GATCA
FspI 149 TGCGCA TGC^GCA
Hin1I 1360 GGCGTC G[GA]^CG[CT]C
HindIII 1069 AAGCTT A^AGCTT
Ksp632I 499 GAAGAG CTCTTC(1/4)
MfeI 1185 CAATTG C^AATTG
MunI 1185 CAATTG C^AATTG
MvnI 1351 CGCG CG^CG
NcoI 158 CCATGG C^CATGG
PaeR7I 1690 CTCGAG C^TCGAG
PflMI 830 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1432 CACGTG CAC^GTG
PmlI 1432 CACGTG CAC^GTG
SacI 361 GAGCTC GAGCT^C
SmaI 596 CCCGGG CCC^GGG
SnaBI 1393 TACGTA TAC^GTA
Van91I 830 CCACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1690 CTCGAG C^TCGAG
XmaCI 596 CCCGGG C^CCGGG
XmaI 596 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 50 enzymes) #
###################################
AcsI 943 GAATTT [GA]^AATT[CT]
1585 GAATTC
AfaI 348 GTAC GT^AC
1645 GTAC
AhdI 1228 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1728 GACCCCAAGTC
AlwI 1097 GGATC GGATC(4/5)
1260 GGATC
AocI 794 CCTCAGG CC^T[ACGT]AGG
1000 CCTGAGG
ApoI 943 GAATTT [GA]^AATT[CT]
1585 GAATTC
AseI 34 ATTAAT AT^TAAT
1678 ATTAAT
AsnI 34 ATTAAT AT^TAAT
1678 ATTAAT
AspEI 1228 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1728 GACCCCAAGTC
AvaI 596 CCCGGG C^[CT]CG[GA]G
1690 CTCGAG
BanI 315 GGCACC G^G[CT][GA]CC
1449 GGCACC
BfaI 1123 CTAG C^TAG
1297 CTAG
BsaAI 1393 TACGTA [CT]AC^GT[GA]
1432 CACGTG
BsiEI 458 CGGCCG CG[GA][CT]^CG
849 CGGCCG
BsmAI 748 GAGAC GTCTC(1/5)
1285 GAGAC
BsoBI 596 CCCGGG C^[CT]CG[GA]G
1690 CTCGAG
BsrFI 765 GCCGGC [GA]^CCGG[CT]
1873 GCCGGC
BstXI 125 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2099 CCAAACGGCTGG
BstYI 1260 GGATCT [GA]^GATC[CT]
1484 AGATCT
Bsu36I 794 CCTCAGG CC^T[ACGT]AGG
1000 CCTGAGG
Cfr10I 765 GCCGGC [GA]^CCGG[CT]
1873 GCCGGC
DraIII 831 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2039 CACGGCGTG
EagI 458 CGGCCG C^GGCCG
849 CGGCCG
Eam1105I 1228 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1728 GACCCCAAGTC
EclXI 458 CGGCCG C^GGCCG
849 CGGCCG
Eco52I 458 CGGCCG C^GGCCG
849 CGGCCG
Eco81I 794 CCTCAGG CC^T[ACGT]AGG
1000 CCTGAGG
EcoNI 178 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1929 CCTCTCAGAGG
HaeII 154 AGCGCC [GA]GCGC^[CT]
1593 AGCGCC
HgaI 1163 GACGC GACGC(5/10)
1361 GCGTC
HincII 1014 GTTGAC GT[CT]^[GA]AC
1180 GTCAAC
HindII 1014 GTTGAC GT[CT]^[GA]AC
1180 GTCAAC
HphI 899 TCACC GGTGA(8/7)
1763 TCACC
MaeI 1123 CTAG C^TAG
1297 CTAG
MaeIII 254 GTGAC ^GT[ACGT]AC
271 GTGAC
MboII 499 GAAGA GAAGA(8/7)
520 TCTTC
MflI 1260 GGATCT [GA]^GATC[CT]
1484 AGATCT
MseI 35 TTAA T^TAA
1679 TTAA
NaeI 765 GCCGGC GCC^GGC
1873 GCCGGC
NgoMIV 765 GCCGGC G^CCGGC
1873 GCCGGC
PshBI 34 ATTAAT AT^TAAT
1678 ATTAAT
PstI 118 CTGCAG CTGCA^G
1270 CTGCAG
RsaI 348 GTAC GT^AC
1645 GTAC
SfcI 118 CTGCAG C^T[GA][CT]AG
1270 CTGCAG
SphI 632 GCATGC GCATG^C
1955 GCATGC
TaqI 746 TCGA T^CGA
1691 TCGA
Tru9I 35 TTAA T^TAA
1679 TTAA
Tsp45I 254 GTGAC ^GT[GC]AC
271 GTGAC
TthHB8I 746 TCGA T^CGA
1691 TCGA
XhoII 1260 GGATCT [GA]^GATC[CT]
1484 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AflIII AluI AlwNI ApaI
AvaII BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfiI SmlI StuI StyI TfiI
TseI Tsp509I TspRI