(Circular) (Six-base) MAPSORT of: pFN21AB8012.seq Check: 7862 from: 1 to: 6829 pFN21AB8012 6829 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3466 3466 Size: 6829 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4172 1937 Size: 2235 4594 Acc65I G'GTAC_C Cuts at: 4150 4150 Size: 6829 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3017 4118 4892 6276 6544 1632 Size: 1385 1101 774 1384 268 1917 Fragments arranged by size: 1917 1385 1384 1101 774 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 3438 3855 3903 5728 6705 1203 Size: 2235 417 48 1825 977 1327 Fragments arranged by size: 2235 1825 1327 977 417 48 AgeI A'CCGG_T Cuts at: 5660 5660 Size: 6829 AhdI GACnn_n'nnGTC Cuts at: 3238 3738 5558 3238 Size: 500 1820 4509 Fragments arranged by size: 4509 1820 500 AleI CACnn'nnGTG Cuts at: 1166 2135 1166 Size: 969 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6829 AlwNI CAG_nnn'CTG Cuts at: 2195 3325 3944 4084 4572 6144 2195 Size: 1130 619 140 488 1572 2880 Fragments arranged by size: 2880 1572 1130 619 488 140 ApaI G_GGCC'C Cuts at: 2333 3694 4120 2333 Size: 1361 426 5042 Fragments arranged by size: 5042 1361 426 ApaLI G'TGCA_C Cuts at: 4886 6042 4886 Size: 1156 5673 ApoI r'AATT_y Cuts at: 1493 2948 3590 4138 4364 4516 6625 1493 Size: 1455 642 548 226 152 2109 1697 Fragments arranged by size: 2109 1697 1455 642 548 226 152 AseI AT'TA_AT Cuts at: 160 1032 2040 3684 5383 160 Size: 872 1008 1644 1699 1606 Fragments arranged by size: 1699 1644 1606 1008 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 2601 3695 4154 1870 Size: 73 658 1094 459 4545 Fragments arranged by size: 4545 1094 658 459 73 AvrII C'CTAG_G Cuts at: 3127 3127 Size: 6829 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2336 2369 2336 Size: 33 6796 BamHI G'GATC_C Cuts at: 4159 4526 4159 Size: 367 6462 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 3454 4150 5605 618 Size: 325 217 627 533 1134 696 1455 1842 Fragments arranged by size: 1842 1455 1134 696 627 533 325 217 BanII G_rGCy'C Cuts at: 1391 2333 2370 2550 3694 3921 4094 4120 4148 Size: 942 37 180 1144 227 173 26 28 Cuts at: 4148 1391 Size: 4072 Fragments arranged by size: 4072 1144 942 227 180 173 37 28 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5858 BbvCI CC'TCA_GC Cuts at: 3041 3041 Size: 6829 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3905 3939 5020 5054 1903 Size: 34 1968 34 1081 34 3678 Fragments arranged by size: 3678 1968 1081 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4721 5931 4721 Size: 1210 5619 BclI T'GATC_A Cuts at: 1548 2415 1548 Size: 867 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2058 2249 2337 Size: 107 122 71 631 605 386 191 88 Cuts at: 2337 2471 4108 5440 136 Size: 134 1637 1332 1525 Fragments arranged by size: 1637 1525 1332 631 605 386 191 134 122 107 88 71 BglII A'GATC_T Cuts at: 3489 6824 3489 Size: 3335 3494 BlpI GC'TnA_GC Cuts at: 4246 4246 Size: 6829 Bme1580I G_kGCm'C Cuts at: 1790 2333 3694 4120 4890 6046 1790 Size: 543 1361 426 770 1156 2573 Fragments arranged by size: 2573 1361 1156 770 543 426 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2833 2961 4121 Size: 416 53 83 399 24 1416 128 1160 Cuts at: 4121 4560 5518 442 Size: 439 958 1753 Fragments arranged by size: 1753 1416 1160 958 439 416 399 128 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6829 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3511 3511 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2573 3302 3697 5489 1239 Size: 1145 189 729 395 1792 2579 Fragments arranged by size: 2579 1792 1145 729 395 189 Bpu10I CC'TnA_GC Cuts at: 2184 2544 3041 2184 Size: 360 497 5972 Fragments arranged by size: 5972 497 360 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2412 2653 3413 4954 5819 6117 2412 Size: 241 760 1541 865 298 3124 Fragments arranged by size: 3124 1541 865 760 298 241 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5492 882 Size: 617 3993 2219 Fragments arranged by size: 3993 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3400 3439 4620 493 Size: 711 2196 39 1181 2702 Fragments arranged by size: 2702 2196 1181 711 39 BsaBI GATnn'nnATC Cuts at: 6823 6823 Size: 6829 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3366 4636 5018 5653 Size: 53 83 186 936 1833 1270 382 635 Cuts at: 5653 275 Size: 1451 Fragments arranged by size: 1833 1451 1270 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5262 5660 5934 6081 6492 5262 Size: 398 274 147 411 5599 Fragments arranged by size: 5599 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3002 3032 1178 Size: 30 165 30 1599 30 4975 Fragments arranged by size: 4975 1599 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 2468 2492 2495 2498 2501 2522 2525 Size: 1123 159 24 3 3 3 21 3 Cuts at: 2525 2528 2906 3023 3080 3083 3164 3167 1186 Size: 3 378 117 57 3 81 3 4848 Fragments arranged by size: 4848 1123 378 159 117 81 57 24 21 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2673 2898 2989 3028 3132 3249 3595 3799 3883 Size: 225 91 39 104 117 346 204 84 Cuts at: 3883 4018 4111 6032 6528 2673 Size: 135 93 1921 496 2974 Fragments arranged by size: 2974 1921 496 346 225 204 135 117 104 93 91 84 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 2670 3314 3968 1163 Size: 573 336 598 644 654 4024 Fragments arranged by size: 4024 654 644 598 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2466 2857 4279 5040 6068 1540 Size: 275 186 465 391 1422 761 1028 2301 Fragments arranged by size: 2301 1422 1028 761 465 391 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 4890 4975 6046 2370 Size: 2520 85 1071 3153 Fragments arranged by size: 3153 2520 1071 85 BsmI GAATG_Cn' Cuts at: 3863 4340 4433 3863 Size: 477 93 6259 Fragments arranged by size: 6259 477 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2747 2747 Size: 6829 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2370 2550 3694 3921 4094 4120 Size: 399 543 37 180 1144 227 173 26 Cuts at: 4120 4148 4890 4975 6046 1391 Size: 28 742 85 1071 2174 Fragments arranged by size: 2174 1144 1071 742 543 399 227 180 173 85 37 28 26 BspEI T'CCGG_A Cuts at: 6492 6492 Size: 6829 BspHI T'CATG_A Cuts at: 4718 6448 4718 Size: 1730 5099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2160 2775 3234 3466 4184 844 Size: 1316 615 459 232 718 3489 Fragments arranged by size: 3489 1316 718 615 459 232 BsrBI CCG'CTC Cuts at: 1394 1823 4716 1394 Size: 429 2893 3507 Fragments arranged by size: 3507 2893 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2278 5324 5498 65 Size: 1183 1030 3046 174 1396 Fragments arranged by size: 3046 1396 1183 1030 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2770 3878 5473 5660 1700 Size: 110 960 1108 1595 187 2869 Fragments arranged by size: 2869 1595 1108 960 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 3405 4889 5901 3405 Size: 1484 1012 4333 Fragments arranged by size: 4333 1484 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4028 794 Size: 3234 3595 BstBI TT'CG_AA Cuts at: 5633 5633 Size: 6829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6829 BstXI CCAn_nnnn'nTGG Cuts at: 2137 4111 2137 Size: 1974 4855 BstYI r'GATC_y Cuts at: 1972 3265 3489 4159 4526 4911 4928 6380 6466 Size: 1293 224 670 367 385 17 1452 86 Cuts at: 6466 6478 6824 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1293 670 385 367 346 224 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2800 3006 2800 Size: 206 6623 BtgI C'CryG_G Cuts at: 513 1065 2053 2163 2247 3083 3837 513 Size: 552 988 110 84 836 754 3505 Fragments arranged by size: 3505 988 836 754 552 110 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3454 487 Size: 683 56 2228 3862 Fragments arranged by size: 3862 2228 683 56 BtsI GCAGTG_nn' Cuts at: 3181 3323 3801 4345 5139 5159 3181 Size: 142 478 544 794 20 4851 Fragments arranged by size: 4851 794 544 478 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6829 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6794 DraI TTT'AAA Cuts at: 4133 4133 Size: 6829 DraIII CAC_nnn'GTG Cuts at: 2841 4049 2841 Size: 1208 5621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5836 809 Size: 507 4520 1802 Fragments arranged by size: 4520 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2463 2854 Size: 54 1425 50 275 18 204 429 391 Cuts at: 2854 3507 3834 4276 5165 6794 8 Size: 653 327 442 889 1629 43 Fragments arranged by size: 1629 1425 889 653 442 429 391 327 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2463 2854 4276 1537 Size: 275 651 391 1422 4090 Fragments arranged by size: 4090 1422 651 391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2499 4759 1353 Size: 1146 2260 3423 Fragments arranged by size: 3423 2260 1146 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2306 5416 5790 5936 387 Size: 1064 138 717 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 717 374 146 138 EcoICRI GAG'CTC Cuts at: 2368 2368 Size: 6829 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2573 3017 3302 3697 4118 4892 Size: 393 752 189 444 285 395 421 774 Cuts at: 4892 5489 6276 6544 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 774 752 597 444 421 395 393 285 268 189 EcoNI CCTnn'n_nnAGG Cuts at: 2187 3938 2187 Size: 1751 5078 EcoO109I rG'GnC_Cy Cuts at: 2060 2141 2149 2192 2330 2405 2566 2577 2805 Size: 81 8 43 138 75 161 11 228 Cuts at: 2805 2977 3690 3691 4116 2060 Size: 172 713 1 425 4773 Fragments arranged by size: 4773 713 425 228 172 161 138 81 75 43 11 8 1 EcoRI G'AATT_C Cuts at: 3590 4138 4516 3590 Size: 548 378 5903 Fragments arranged by size: 5903 548 378 FspI TGC'GCA Cuts at: 2156 5335 2156 Size: 3179 3650 HaeII r_GCGC'y Cuts at: 1678 2163 3602 5976 1678 Size: 485 1439 2374 2531 Fragments arranged by size: 2531 2374 1439 485 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2539 2651 2683 3224 Size: 32 135 32 935 32 112 32 541 Cuts at: 3224 3256 3724 3756 5470 5502 5544 5576 1373 Size: 32 468 32 1714 32 42 32 2626 Fragments arranged by size: 2626 1714 935 541 468 135 112 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3187 4173 4419 669 Size: 988 281 1083 166 986 246 3079 Fragments arranged by size: 3079 1083 988 986 281 246 166 HindIII A'AGCT_T Cuts at: 748 3074 748 Size: 2326 4503 HpaI GTT'AAC Cuts at: 4419 4419 Size: 6829 KpnI G_GTAC'C Cuts at: 4154 4154 Size: 6829 MfeI C'AATT_G Cuts at: 3190 4428 3190 Size: 1238 5591 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2787 5943 6127 1305 Size: 1482 3156 184 2007 Fragments arranged by size: 3156 2007 1482 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5007 Fragments arranged by size: 5007 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3356 3747 4787 5146 5305 518 Size: 648 969 1221 391 1040 359 159 2042 Fragments arranged by size: 2042 1221 1040 969 648 391 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2135 3280 3528 4245 4922 6070 6315 Size: 501 28 1145 248 717 677 1148 245 Cuts at: 6315 1606 Size: 2120 Fragments arranged by size: 2120 1148 1145 717 677 501 248 245 28 NaeI GCC'GGC Cuts at: 2772 3880 2772 Size: 1108 5721 NcoI C'CATG_G Cuts at: 513 1065 2163 513 Size: 552 1098 5179 Fragments arranged by size: 5179 1098 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6829 NgoMIV G'CCGG_C Cuts at: 2770 3878 2770 Size: 1108 5721 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6829 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 2641 3907 3964 4187 5732 1132 Size: 97 1412 1266 57 223 1545 2229 Fragments arranged by size: 2229 1545 1412 1266 223 97 57 PasI CC'CwG_GG Cuts at: 1348 2534 2689 3098 1348 Size: 1186 155 409 5079 Fragments arranged by size: 5079 1186 409 155 PciI A'CATG_T Cuts at: 3903 5728 3903 Size: 1825 5004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2841 1108 Size: 1733 5096 PfoI T'CCnGG_A Cuts at: 2790 2790 Size: 6829 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PmlI CAC'GTG Cuts at: 3439 3439 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4898 4930 6411 6443 4898 Size: 32 1481 32 5284 Fragments arranged by size: 5284 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2149 2405 2805 2141 Size: 8 256 400 6165 Fragments arranged by size: 6165 400 256 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6829 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PspOMI G'GGCC_C Cuts at: 2329 3690 4116 2329 Size: 1361 426 5042 Fragments arranged by size: 5042 1361 426 PspXI vC'TCGA_Gb Cuts at: 3695 3695 Size: 6829 PstI C_TGCA'G Cuts at: 830 2127 3279 4181 830 Size: 1297 1152 902 3478 Fragments arranged by size: 3478 1297 1152 902 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 PvuII CAG'CTG Cuts at: 2107 2135 3280 2107 Size: 28 1145 5656 Fragments arranged by size: 5656 1145 28 SacI G_AGCT'C Cuts at: 2370 2370 Size: 6829 SalI G'TCGA_C Cuts at: 1936 4171 1936 Size: 2235 4594 SanDI GG'GwC_CC Cuts at: 2141 2141 Size: 6829 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 5077 6789 5077 Size: 1712 5117 SfcI C'TryA_G Cuts at: 826 1045 2123 3275 4177 5312 5993 6184 826 Size: 219 1078 1152 902 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 1078 902 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2249 2337 2471 2249 Size: 88 134 6607 Fragments arranged by size: 6607 134 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 2603 4156 1872 Size: 731 1553 4545 Fragments arranged by size: 4545 1553 731 SmlI C'TyrA_G Cuts at: 820 1017 1943 2427 2668 3428 3695 4933 5834 Size: 197 926 484 241 760 267 1238 901 Cuts at: 5834 6096 820 Size: 262 1553 Fragments arranged by size: 1553 1238 926 901 760 484 267 262 241 197 SnaBI TAC'GTA Cuts at: 493 3400 4620 493 Size: 2907 1220 2702 Fragments arranged by size: 2907 2702 1220 SpeI A'CTAG_T Cuts at: 152 152 Size: 6829 SphI G_CATG'C Cuts at: 1132 2641 3964 4187 1132 Size: 1509 1323 223 3774 Fragments arranged by size: 3774 1509 1323 223 SspI AAT'ATT Cuts at: 5 52 4753 5 Size: 47 4701 2081 Fragments arranged by size: 4701 2081 47 StuI AGG'CCT Cuts at: 1517 2340 2397 4001 1517 Size: 823 57 1604 4345 Fragments arranged by size: 4345 1604 823 57 StyI C'CwwG_G Cuts at: 513 1065 2145 2163 2286 2325 3127 4268 513 Size: 552 1080 18 123 39 802 1141 3074 Fragments arranged by size: 3074 1141 1080 802 552 123 39 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2852 3161 4856 4873 5026 5211 5656 Size: 1255 1272 309 1695 17 153 185 445 Cuts at: 5656 325 Size: 1498 Fragments arranged by size: 1695 1498 1272 1255 445 309 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 5075 6787 Size: 274 80 33 51 1443 375 2723 1712 Cuts at: 6787 96 Size: 138 Fragments arranged by size: 2723 1712 1443 375 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6789 6821 6789 Size: 32 6797 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6829 XbaI T'CTAG_A Cuts at: 4165 4165 Size: 6829 XhoI C'TCGA_G Cuts at: 1943 3695 1943 Size: 1752 5077 XmaI C'CCGG_G Cuts at: 1870 2601 4154 1870 Size: 731 1553 4545 Fragments arranged by size: 4545 1553 731 XmnI GAAnn'nnTTC Cuts at: 4958 4958 Size: 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI XcmI