Restriction map for
pFN21AB8012
(Linear) (Six-base) MAPSORT of: KIBB8012.seq Check: 7489 from: 1 to: 2124
KIBB8012.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1461 2124
Size: 1461 663
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 2113 2124
Size: 1012 1101 11
Fragments arranged by size:
1101 1012 11
AflIII A'CryG_T
Cuts at: 0 1433 1850 1898 2124
Size: 1433 417 48 226
Fragments arranged by size:
1433 417 226 48
AhdI GACnn_n'nnGTC
Cuts at: 0 1233 1733 2124
Size: 1233 500 391
Fragments arranged by size:
1233 500 391
AleI CACnn'nnGTG
Cuts at: 0 130 2124
Size: 130 1994
AlwNI CAG_nnn'CTG
Cuts at: 0 190 1320 1939 2079 2124
Size: 190 1130 619 140 45
Fragments arranged by size:
1130 619 190 140 45
ApaI G_GGCC'C
Cuts at: 0 328 1689 2115 2124
Size: 328 1361 426 9
Fragments arranged by size:
1361 426 328 9
ApoI r'AATT_y
Cuts at: 0 943 1585 2124
Size: 943 642 539
Fragments arranged by size:
943 642 539
AseI AT'TA_AT
Cuts at: 0 35 1679 2124
Size: 35 1644 445
Fragments arranged by size:
1644 445 35
AvaI C'yCGr_G
Cuts at: 0 596 1690 2124
Size: 596 1094 434
Fragments arranged by size:
1094 596 434
AvrII C'CTAG_G
Cuts at: 0 1122 2124
Size: 1122 1002
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 331 364 2124
Size: 331 33 1760
Fragments arranged by size:
1760 331 33
BanI G'GyrC_C
Cuts at: 0 315 1449 2124
Size: 315 1134 675
Fragments arranged by size:
1134 675 315
BanII G_rGCy'C
Cuts at: 0 328 365 545 1689 1916 2089 2115 2124
Size: 328 37 180 1144 227 173 26 9
Fragments arranged by size:
1144 328 227 180 173 37 26 9
BbvCI CC'TCA_GC
Cuts at: 0 1036 2124
Size: 1036 1088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1900 1934 2124
Size: 1900 34 190
Fragments arranged by size:
1900 190 34
BclI T'GATC_A
Cuts at: 0 410 2124
Size: 410 1714
BglI GCCn_nnn'nGGC
Cuts at: 0 53 244 332 466 2103 2124
Size: 53 191 88 134 1637 21
Fragments arranged by size:
1637 191 134 88 53 21
BglII A'GATC_T
Cuts at: 0 1484 2124
Size: 1484 640
Bme1580I G_kGCm'C
Cuts at: 0 328 1689 2115 2124
Size: 328 1361 426 9
Fragments arranged by size:
1361 426 328 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 828 956 2124
Size: 828 128 1168
Fragments arranged by size:
1168 828 128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1506 2124
Size: 1506 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 568 1297 1692 2124
Size: 379 189 729 395 432
Fragments arranged by size:
729 432 395 379 189
Bpu10I CC'TnA_GC
Cuts at: 0 179 539 1036 2124
Size: 179 360 497 1088
Fragments arranged by size:
1088 497 360 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 648 1408 2124
Size: 407 241 760 716
Fragments arranged by size:
760 716 407 241
BsaAI yAC'GTr
Cuts at: 0 1395 1434 2124
Size: 1395 39 690
Fragments arranged by size:
1395 690 39
BsaHI Gr'CG_yC
Cuts at: 0 1361 2124
Size: 1361 763
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 997 1027 2124
Size: 997 30 1097
Fragments arranged by size:
1097 997 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 463 487 490 493 496 517 520
Size: 304 159 24 3 3 3 21 3
Cuts at: 520 523 901 1018 1075 1078 1159 1162 2124
Size: 3 378 117 57 3 81 3 962
Fragments arranged by size:
962 378 304 159 117 81 57 24
21 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 668 893 984 1023 1127 1244 1590 1794
Size: 668 225 91 39 104 117 346 204
Cuts at: 1794 1878 2013 2106 2124
Size: 84 135 93 18
Fragments arranged by size:
668 346 225 204 135 117 104 93
91 84 39 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 665 1309 1963 2124
Size: 67 598 644 654 161
Fragments arranged by size:
654 644 598 161 67
BsiEI CG_ry'CG
Cuts at: 0 461 852 2124
Size: 461 391 1272
Fragments arranged by size:
1272 461 391
BsiHKAI G_wGCw'C
Cuts at: 0 365 2124
Size: 365 1759
BsmI GAATG_Cn'
Cuts at: 0 1858 2124
Size: 1858 266
BsmBI CGTCTCn'nnnn_
Cuts at: 0 742 2124
Size: 742 1382
Bsp1286I G_dGCh'C
Cuts at: 0 328 365 545 1689 1916 2089 2115 2124
Size: 328 37 180 1144 227 173 26 9
Fragments arranged by size:
1144 328 227 180 173 37 26 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 770 1229 1461 2124
Size: 155 615 459 232 663
Fragments arranged by size:
663 615 459 232 155
BsrDI GCAATG_nn'
Cuts at: 0 273 2124
Size: 273 1851
BsrFI r'CCGG_y
Cuts at: 0 765 1873 2124
Size: 765 1108 251
Fragments arranged by size:
1108 765 251
BssSI C'ACGA_G
Cuts at: 0 1400 2124
Size: 1400 724
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2023 2124
Size: 2023 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 2106 2124
Size: 132 1974 18
Fragments arranged by size:
1974 132 18
BstYI r'GATC_y
Cuts at: 0 1260 1484 2124
Size: 1260 224 640
Fragments arranged by size:
1260 640 224
Bsu36I CC'TnA_GG
Cuts at: 0 795 1001 2124
Size: 795 206 1123
Fragments arranged by size:
1123 795 206
BtgI C'CryG_G
Cuts at: 0 48 158 242 1078 1832 2124
Size: 48 110 84 836 754 292
Fragments arranged by size:
836 754 292 110 84 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1449 2124
Size: 1449 675
BtsI GCAGTG_nn'
Cuts at: 0 1176 1318 1796 2124
Size: 1176 142 478 328
Fragments arranged by size:
1176 478 328 142
DraIII CAC_nnn'GTG
Cuts at: 0 836 2044 2124
Size: 836 1208 80
Fragments arranged by size:
1208 836 80
EaeI y'GGCC_r
Cuts at: 0 29 458 849 1502 1829 2124
Size: 29 429 391 653 327 295
Fragments arranged by size:
653 429 391 327 295 29
EagI C'GGCC_G
Cuts at: 0 458 849 2124
Size: 458 391 1275
Fragments arranged by size:
1275 458 391
EarI CTCTTCn'nnn_
Cuts at: 0 494 2124
Size: 494 1630
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 301 2124
Size: 301 1823
EcoICRI GAG'CTC
Cuts at: 0 363 2124
Size: 363 1761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 568 1012 1297 1692 2113 2124
Size: 379 189 444 285 395 421 11
Fragments arranged by size:
444 421 395 379 285 189 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 182 1933 2124
Size: 182 1751 191
Fragments arranged by size:
1751 191 182
EcoO109I rG'GnC_Cy
Cuts at: 0 55 136 144 187 325 400 561 572
Size: 55 81 8 43 138 75 161 11
Cuts at: 572 800 972 1685 1686 2111 2124
Size: 228 172 713 1 425 13
Fragments arranged by size:
713 425 228 172 161 138 81 75
55 43 13 11 8 1
EcoRI G'AATT_C
Cuts at: 0 1585 2124
Size: 1585 539
FspI TGC'GCA
Cuts at: 0 151 2124
Size: 151 1973
HaeII r_GCGC'y
Cuts at: 0 158 1597 2124
Size: 158 1439 527
Fragments arranged by size:
1439 527 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 646 678 1219 1251 1719 1751
Size: 502 32 112 32 541 32 468 32
Cuts at: 1751 2124
Size: 373
Fragments arranged by size:
541 502 468 373 112 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1016 1182 2124
Size: 1016 166 942
Fragments arranged by size:
1016 942 166
HindIII A'AGCT_T
Cuts at: 0 1069 2124
Size: 1069 1055
MfeI C'AATT_G
Cuts at: 0 1185 2124
Size: 1185 939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2124
Size: 782 1342
MslI CAynn'nnrTG
Cuts at: 0 130 1351 1742 2124
Size: 130 1221 391 382
Fragments arranged by size:
1221 391 382 130
MspA1I CmG'CkG
Cuts at: 0 102 130 1275 1523 2124
Size: 102 28 1145 248 601
Fragments arranged by size:
1145 601 248 102 28
NaeI GCC'GGC
Cuts at: 0 767 1875 2124
Size: 767 1108 249
Fragments arranged by size:
1108 767 249
NcoI C'CATG_G
Cuts at: 0 158 2124
Size: 158 1966
NgoMIV G'CCGG_C
Cuts at: 0 765 1873 2124
Size: 765 1108 251
Fragments arranged by size:
1108 765 251
NspI r_CATG'y
Cuts at: 0 636 1902 1959 2124
Size: 636 1266 57 165
Fragments arranged by size:
1266 636 165 57
PasI CC'CwG_GG
Cuts at: 0 529 684 1093 2124
Size: 529 155 409 1031
Fragments arranged by size:
1031 529 409 155
PciI A'CATG_T
Cuts at: 0 1898 2124
Size: 1898 226
PflMI CCAn_nnn'nTGG
Cuts at: 0 836 2124
Size: 836 1288
PfoI T'CCnGG_A
Cuts at: 0 785 2124
Size: 785 1339
PmlI CAC'GTG
Cuts at: 0 1434 2124
Size: 1434 690
PpuMI rG'GwC_Cy
Cuts at: 0 136 144 400 800 2124
Size: 136 8 256 400 1324
Fragments arranged by size:
1324 400 256 136 8
PspOMI G'GGCC_C
Cuts at: 0 324 1685 2111 2124
Size: 324 1361 426 13
Fragments arranged by size:
1361 426 324 13
PspXI vC'TCGA_Gb
Cuts at: 0 1690 2124
Size: 1690 434
PstI C_TGCA'G
Cuts at: 0 122 1274 2124
Size: 122 1152 850
Fragments arranged by size:
1152 850 122
PvuII CAG'CTG
Cuts at: 0 102 130 1275 2124
Size: 102 28 1145 849
Fragments arranged by size:
1145 849 102 28
SacI G_AGCT'C
Cuts at: 0 365 2124
Size: 365 1759
SanDI GG'GwC_CC
Cuts at: 0 136 2124
Size: 136 1988
SfcI C'TryA_G
Cuts at: 0 118 1270 2124
Size: 118 1152 854
Fragments arranged by size:
1152 854 118
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 244 332 466 2124
Size: 244 88 134 1658
Fragments arranged by size:
1658 244 134 88
SmaI CCC'GGG
Cuts at: 0 598 2124
Size: 598 1526
SmlI C'TyrA_G
Cuts at: 0 422 663 1423 1690 2124
Size: 422 241 760 267 434
Fragments arranged by size:
760 434 422 267 241
SnaBI TAC'GTA
Cuts at: 0 1395 2124
Size: 1395 729
SphI G_CATG'C
Cuts at: 0 636 1959 2124
Size: 636 1323 165
Fragments arranged by size:
1323 636 165
StuI AGG'CCT
Cuts at: 0 335 392 1996 2124
Size: 335 57 1604 128
Fragments arranged by size:
1604 335 128 57
StyI C'CwwG_G
Cuts at: 0 140 158 281 320 1122 2124
Size: 140 18 123 39 802 1002
Fragments arranged by size:
1002 802 140 123 39 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 847 1156 2124
Size: 847 309 968
Fragments arranged by size:
968 847 309
TatI w'GTAC_w
Cuts at: 0 347 2124
Size: 347 1777
XhoI C'TCGA_G
Cuts at: 0 1690 2124
Size: 1690 434
XmaI C'CCGG_G
Cuts at: 0 596 2124
Size: 596 1528
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApoI
AseI AvaI AvrII BaeI BanI BanII BbvCI BcgI
BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII MfeI MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SanDI SfcI SfiI SmaI SmlI SnaBI SphI
StuI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI AscI AsiSI BamHI BbeI BbsI BciVI
BfrBI BlpI BmgBI BmtI BsaI BsaBI BsaWI BsiWI
BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstZ17I
ClaI CspCI DraI DrdI EcoRV FalI FseI FspAI
HpaI KasI KpnI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XmnI ZraI