################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BcgI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1288 GACGTC GACGT^C AccI 1858 GTAGAC GT^[AC][GT]AC AcsI 1952 AAATTC [GA]^AATT[CT] Alw44I 166 GTGCAC G^TGCAC ApaI 2217 GGGCCC GGGCC^C ApaLI 166 GTGCAC G^TGCAC ApoI 1952 AAATTC [GA]^AATT[CT] Asp700I 1632 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 166 GTGCAC G[AT]GC[AT]^C BalI 135 TGGCCA TGG^CCA BbeI 1669 GGCGCC GGCGC^C BbrPI 1278 CACGTG CAC^GTG BbvCI 1214 CCTCAGC CCTCAGC(-5/-2) BciVI 770 GTATCC GTATCC(6/5) BsaAI 1278 CACGTG [CT]AC^GT[GA] BsiHKAI 166 GTGCAC G[AT]GC[AT]^C BsrFI 315 GCCGGC [GA]^CCGG[CT] BssSI 1414 CTCGTG CACGAG(-5/-1) BstEII 1347 GGTCACC G^GT[ACGT]ACC BstPI 1347 GGTCACC G^GT[ACGT]ACC Cfr10I 315 GCCGGC [GA]^CCGG[CT] EaeI 135 TGGCCA [CT]^GGCC[GA] EcoNI 1173 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1347 GGTCACC G^GT[ACGT]ACC FseI 314 GGCCGGCC GGCCGG^CC KasI 1669 GGCGCC G^GCGCC MluNI 135 TGGCCA TGG^CCA MscI 135 TGGCCA TGG^CCA NaeI 315 GCCGGC GCC^GGC NarI 1669 GGCGCC GG^CGCC NcoI 681 CCATGG C^CATGG NgoMIV 315 GCCGGC G^CCGGC PmaCI 1278 CACGTG CAC^GTG PmlI 1278 CACGTG CAC^GTG PshAI 1437 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 81 GCTCTTC GCTCTTC(1/4) SfoI 1669 GGCGCC GGC^GCC SmaI 483 CCCGGG CCC^GGG SmlI 1993 CTCAAG C^T[CT][GA]AG SphI 1076 GCATGC GCATG^C XcmI 1049 CCATAGGAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 483 CCCGGG C^CCGGG XmaI 483 CCCGGG C^CCGGG XmnI 1632 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 1288 GACGTC G[GA]^CG[CT]C 1669 GGCGCC AlwNI 397 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 726 CAGCTTCTG AocI 607 CCTGAGG CC^T[ACGT]AGG 1957 CCTGAGG AvaI 483 CCCGGG C^[CT]CG[GA]G 1751 CCCGAG BamHI 1692 GGATCC G^GATCC 2187 GGATCC BanI 461 GGCACC G^G[CT][GA]CC 1669 GGCGCC BfaI 2158 CTAG C^TAG 2320 CTAG BglI 300 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1643 GCCCCAGTGGC BglII 1430 AGATCT A^GATCT 2348 AGATCT BsaHI 1288 GACGTC G[GA]^CG[CT]C 1669 GGCGCC BsmAI 202 GAGAC GTCTC(1/5) 1594 GTCTC BsmI 252 GAATGC GAATGC(1/-1) 1419 GAATGC BsoBI 483 CCCGGG C^[CT]CG[GA]G 1751 CCCGAG Bsu36I 607 CCTGAGG CC^T[ACGT]AGG 1957 CCTGAGG EarI 82 CTCTTC CTCTTC(1/4) 199 GAAGAG Eco81I 607 CCTGAGG CC^T[ACGT]AGG 1957 CCTGAGG Hin1I 1288 GACGTC G[GA]^CG[CT]C 1669 GGCGCC Ksp632I 82 CTCTTC CTCTTC(1/4) 199 GAAGAG MaeI 2158 CTAG C^TAG 2320 CTAG MseI 1252 TTAA T^TAA 1477 TTAA PflMI 1160 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1427 CCAAGATCTGG TfiI 1494 GATTC G^A[AT]TC 2161 GAATC Tru9I 1252 TTAA T^TAA 1477 TTAA Tsp509I 71 AATT ^AATT 1953 AATT Van91I 1160 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1427 CCAAGATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI XhoII