(Linear) (Six-base) MAPSORT of: KIEE4579.seq Check: 1588 from: 1 to: 2355 KIEE4579.orf With 182 enzymes: SgfI * March 22, 2012 18:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 64 336 1251 2355 Size: 64 272 915 1104 Fragments arranged by size: 1104 915 272 64 AatII G_ACGT'C Cuts at: 0 1292 2355 Size: 1292 1063 AccI GT'mk_AC Cuts at: 0 1859 2355 Size: 1859 496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 1107 1159 1350 1585 1651 2355 Size: 259 848 52 191 235 66 704 Fragments arranged by size: 848 704 259 235 191 66 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 239 2355 Size: 239 2116 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 563 595 2355 Size: 563 32 1760 Fragments arranged by size: 1760 563 32 AlwNI CAG_nnn'CTG Cuts at: 0 402 731 2355 Size: 402 329 1624 Fragments arranged by size: 1624 402 329 ApaI G_GGCC'C Cuts at: 0 2221 2355 Size: 2221 134 ApaLI G'TGCA_C Cuts at: 0 166 2355 Size: 166 2189 ApoI r'AATT_y Cuts at: 0 1952 2355 Size: 1952 403 AvaI C'yCGr_G Cuts at: 0 483 1751 2355 Size: 483 1268 604 Fragments arranged by size: 1268 604 483 BamHI G'GATC_C Cuts at: 0 1692 2187 2355 Size: 1692 495 168 Fragments arranged by size: 1692 495 168 BanI G'GyrC_C Cuts at: 0 461 1669 2355 Size: 461 1208 686 Fragments arranged by size: 1208 686 461 BanII G_rGCy'C Cuts at: 0 83 484 1752 2221 2355 Size: 83 401 1268 469 134 Fragments arranged by size: 1268 469 401 134 83 BbeI G_GCGC'C Cuts at: 0 1673 2355 Size: 1673 682 BbsI GAAGACnn'nnnn_ Cuts at: 0 889 1299 1573 2019 2355 Size: 889 410 274 446 336 Fragments arranged by size: 889 446 410 336 274 BbvCI CC'TCA_GC Cuts at: 0 1215 2355 Size: 1215 1140 BciVI GTATCCnnnnn_n' Cuts at: 0 781 2355 Size: 781 1574 BglI GCCn_nnn'nGGC Cuts at: 0 306 1649 2355 Size: 306 1343 706 Fragments arranged by size: 1343 706 306 BglII A'GATC_T Cuts at: 0 1430 2348 2355 Size: 1430 918 7 Fragments arranged by size: 1430 918 7 Bme1580I G_kGCm'C Cuts at: 0 145 170 284 311 719 1186 2221 2355 Size: 145 25 114 27 408 467 1035 134 Fragments arranged by size: 1035 467 408 145 134 114 27 25 BmrI ACTGGGnnnn_n' Cuts at: 0 539 1176 1640 2355 Size: 539 637 464 715 Fragments arranged by size: 715 637 539 464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 343 1428 1537 1570 1594 2278 2292 Size: 229 114 1085 109 33 24 684 14 Cuts at: 2292 2355 Size: 63 Fragments arranged by size: 1085 684 229 114 109 63 33 24 14 Bpu10I CC'TnA_GC Cuts at: 0 406 1215 2355 Size: 406 809 1140 Fragments arranged by size: 1140 809 406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1978 2355 Size: 1978 377 BsaAI yAC'GTr Cuts at: 0 1280 2355 Size: 1280 1075 BsaHI Gr'CG_yC Cuts at: 0 1289 1670 2355 Size: 1289 381 685 Fragments arranged by size: 1289 685 381 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 343 373 1015 1045 1510 1540 2355 Size: 343 30 642 30 465 30 815 Fragments arranged by size: 815 642 465 343 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1081 1387 2355 Size: 1081 306 968 Fragments arranged by size: 1081 968 306 BseYI C'CCAG_C Cuts at: 0 158 304 364 860 1736 2355 Size: 158 146 60 496 876 619 Fragments arranged by size: 876 619 496 158 146 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 716 1176 1358 1874 1897 2355 Size: 716 460 182 516 23 458 Fragments arranged by size: 716 516 460 458 182 23 BsiHKAI G_wGCw'C Cuts at: 0 170 2355 Size: 170 2185 BsmI GAATG_Cn' Cuts at: 0 258 1425 2355 Size: 258 1167 930 Fragments arranged by size: 1167 930 258 Bsp1286I G_dGCh'C Cuts at: 0 83 145 170 284 311 484 719 1186 Size: 83 62 25 114 27 173 235 467 Cuts at: 1186 1752 2221 2355 Size: 566 469 134 Fragments arranged by size: 566 469 467 235 173 134 114 83 62 27 25 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 64 336 757 1251 1919 2261 2355 Size: 64 272 421 494 668 342 94 Fragments arranged by size: 668 494 421 342 272 94 64 BsrDI GCAATG_nn' Cuts at: 0 463 528 587 1825 2355 Size: 463 65 59 1238 530 Fragments arranged by size: 1238 530 463 65 59 BsrFI r'CCGG_y Cuts at: 0 315 2355 Size: 315 2040 BssSI C'ACGA_G Cuts at: 0 1414 2355 Size: 1414 941 BstAPI GCAn_nnn'nTGC Cuts at: 0 21 402 1190 2355 Size: 21 381 788 1165 Fragments arranged by size: 1165 788 381 21 BstEII G'GTnAC_C Cuts at: 0 1347 2355 Size: 1347 1008 BstXI CCAn_nnnn'nTGG Cuts at: 0 304 907 955 2355 Size: 304 603 48 1400 Fragments arranged by size: 1400 603 304 48 BstYI r'GATC_y Cuts at: 0 1430 1692 2187 2348 2355 Size: 1430 262 495 161 7 Fragments arranged by size: 1430 495 262 161 7 Bsu36I CC'TnA_GG Cuts at: 0 608 1958 2355 Size: 608 1350 397 Fragments arranged by size: 1350 608 397 BtgI C'CryG_G Cuts at: 0 681 737 1609 2355 Size: 681 56 872 746 Fragments arranged by size: 872 746 681 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 705 747 1746 2355 Size: 705 42 999 609 Fragments arranged by size: 999 705 609 42 BtsI GCAGTG_nn' Cuts at: 0 818 1592 2355 Size: 818 774 763 Fragments arranged by size: 818 774 763 EaeI y'GGCC_r Cuts at: 0 135 2355 Size: 135 2220 EarI CTCTTCn'nnn_ Cuts at: 0 88 194 2355 Size: 88 106 2161 Fragments arranged by size: 2161 106 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 235 2355 Size: 235 2120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 259 343 1107 1159 1350 1428 1537 Size: 229 30 84 764 52 191 78 109 Cuts at: 1537 1570 1585 1594 1651 2278 2292 2355 Size: 33 15 9 57 627 14 63 Fragments arranged by size: 764 627 229 191 109 84 78 63 57 52 33 30 15 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1177 2355 Size: 1177 1178 EcoO109I rG'GnC_Cy Cuts at: 0 1170 1211 1521 1757 2217 2218 2355 Size: 1170 41 310 236 460 1 137 Fragments arranged by size: 1170 460 310 236 137 41 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 936 2355 Size: 936 1419 FseI GG_CCGG'CC Cuts at: 0 319 2355 Size: 319 2036 HaeII r_GCGC'y Cuts at: 0 1228 1673 2297 2355 Size: 1228 445 624 58 Fragments arranged by size: 1228 624 445 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 613 645 927 959 2146 2178 2355 Size: 613 32 282 32 1187 32 177 Fragments arranged by size: 1187 613 282 177 32 32 32 KasI G'GCGC_C Cuts at: 0 1669 2355 Size: 1669 686 MscI TGG'CCA Cuts at: 0 137 2355 Size: 137 2218 MslI CAynn'nnrTG Cuts at: 0 32 499 659 967 2355 Size: 32 467 160 308 1388 Fragments arranged by size: 1388 467 308 160 32 MspA1I CmG'CkG Cuts at: 0 854 860 1219 1237 1827 2355 Size: 854 6 359 18 590 528 Fragments arranged by size: 854 590 528 359 18 6 NaeI GCC'GGC Cuts at: 0 317 2355 Size: 317 2038 NarI GG'CG_CC Cuts at: 0 1670 2355 Size: 1670 685 NcoI C'CATG_G Cuts at: 0 681 2355 Size: 681 1674 NgoMIV G'CCGG_C Cuts at: 0 315 2355 Size: 315 2040 NspI r_CATG'y Cuts at: 0 1080 1582 2049 2355 Size: 1080 502 467 306 Fragments arranged by size: 1080 502 467 306 PasI CC'CwG_GG Cuts at: 0 42 98 2355 Size: 42 56 2257 Fragments arranged by size: 2257 56 42 PflMI CCAn_nnn'nTGG Cuts at: 0 1166 1433 2355 Size: 1166 267 922 Fragments arranged by size: 1166 922 267 PfoI T'CCnGG_A Cuts at: 0 581 2355 Size: 581 1774 PmlI CAC'GTG Cuts at: 0 1280 2355 Size: 1280 1075 PpuMI rG'GwC_Cy Cuts at: 0 1170 1211 1521 1757 2355 Size: 1170 41 310 236 598 Fragments arranged by size: 1170 598 310 236 41 PshAI GACnn'nnGTC Cuts at: 0 1441 2355 Size: 1441 914 PspOMI G'GGCC_C Cuts at: 0 2217 2355 Size: 2217 138 PstI C_TGCA'G Cuts at: 0 859 1224 1262 2355 Size: 859 365 38 1093 Fragments arranged by size: 1093 859 365 38 PvuII CAG'CTG Cuts at: 0 854 860 1219 1237 1827 2355 Size: 854 6 359 18 590 528 Fragments arranged by size: 854 590 528 359 18 6 SapI GCTCTTCn'nnn_ Cuts at: 0 88 2355 Size: 88 2267 SfcI C'TryA_G Cuts at: 0 600 849 855 1220 1258 1761 2355 Size: 600 249 6 365 38 503 594 Fragments arranged by size: 600 594 503 365 249 38 6 SfoI GGC'GCC Cuts at: 0 1671 2355 Size: 1671 684 SmaI CCC'GGG Cuts at: 0 485 2355 Size: 485 1870 SmlI C'TyrA_G Cuts at: 0 1993 2355 Size: 1993 362 SphI G_CATG'C Cuts at: 0 1080 2355 Size: 1080 1275 SrfI GCCC'GGGC Cuts at: 0 485 2355 Size: 485 1870 StyI C'CwwG_G Cuts at: 0 227 681 2138 2355 Size: 227 454 1457 217 Fragments arranged by size: 1457 454 227 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 609 1622 2355 Size: 609 1013 733 Fragments arranged by size: 1013 733 609 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1510 1542 2355 Size: 1510 32 813 Fragments arranged by size: 1510 813 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1056 2355 Size: 1056 1299 XmaI C'CCGG_G Cuts at: 0 483 2355 Size: 483 1872 XmnI GAAnn'nnTTC Cuts at: 0 1636 2355 Size: 1636 719 ZraI GAC'GTC Cuts at: 0 1290 2355 Size: 1290 1065 Enzymes that do cut: AarI AatII AccI AcuI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SapI SfcI SfoI SmaI SmlI SphI SrfI StyI TaqII TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI Tth111I XbaI XhoI