################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 205 CGCG CG^CG ApaI 887 GGGCCC GGGCC^C AviII 969 TGCGCA TGC^GCA BalI 998 TGGCCA TGG^CCA BbvCI 476 CCTCAGC CCTCAGC(-5/-2) BcnI 27 CCCGG CC^[GC]GG BpmI 815 CTGGAG CTGGAG(16/14) BsmBI 1074 CGTCTC CGTCTC(1/5) BspHI 735 TCATGA T^CATGA BsrBI 1035 GAGCGG CCGCTC(-3/-3) BsrDI 117 CATTGC GCAATG(2/0) BsrFI 164 ACCGGC [GA]^CCGG[CT] BstEII 699 GGTCACC G^GT[ACGT]ACC BstPI 699 GGTCACC G^GT[ACGT]ACC BstUI 205 CGCG CG^CG BstYI 569 AGATCC [GA]^GATC[CT] Cfr10I 164 ACCGGC [GA]^CCGG[CT] DraII 887 GGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 749 CCATGG C^C[GA][CT]GG EagI 906 CGGCCG C^GGCCG EclXI 906 CGGCCG C^GGCCG Eco52I 906 CGGCCG C^GGCCG EcoO109I 887 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 699 GGTCACC G^GT[ACGT]ACC FspI 969 TGCGCA TGC^GCA MflI 569 AGATCC [GA]^GATC[CT] MluNI 998 TGGCCA TGG^CCA MscI 998 TGGCCA TGG^CCA MseI 860 TTAA T^TAA MvnI 205 CGCG CG^CG NciI 27 CCCGG CC^[GC]GG NcoI 749 CCATGG C^CATGG PflMI 375 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 735 TCATGA T^CATGA SbfI 915 CCTGCAGG CCTGCA^GG Sse8387I 915 CCTGCAGG CCTGCA^GG Tru9I 860 TTAA T^TAA Van91I 375 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 569 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 325 GTAGAC GT^[AC][GT]AC 637 GTCTAC AcyI 272 GGCGTC G[GA]^CG[CT]C 837 GGCGTC AfaI 411 GTAC GT^AC 1026 GTAC AlwI 570 GATCC GGATC(4/5) 800 GGATC AlwNI 181 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 525 CAGTATCTG AvaII 339 GGTCC G^G[AT]CC 585 GGACC BglI 913 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 928 GCCCTGAAGGC BsaHI 272 GGCGTC G[GA]^CG[CT]C 837 GGCGTC BstXI 503 CCAATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 534 CCAGCAGAATGG DpnII 570 GATC ^GATC 801 GATC EaeI 906 CGGCCG [CT]^GGCC[GA] 998 TGGCCA EarI 235 CTCTTC CTCTTC(1/4) 1069 CTCTTC FauI 787 CCCGC CCCGC(4/6) 944 GCGGG HapII 28 CCGG C^CGG 165 CCGG HgaI 273 GCGTC GACGC(5/10) 838 GCGTC Hin1I 272 GGCGTC G[GA]^CG[CT]C 837 GGCGTC HinfI 103 GAGTC G^A[ACGT]TC 1063 GAGTC HpaII 28 CCGG C^CGG 165 CCGG Ksp632I 235 CTCTTC CTCTTC(1/4) 1069 CTCTTC MboI 570 GATC ^GATC 801 GATC MspI 28 CCGG C^CGG 165 CCGG NdeII 570 GATC ^GATC 801 GATC PleI 103 GAGTC GAGTC(4/5) 1063 GAGTC PvuII 181 CAGCTG CAG^CTG 194 CAGCTG RsaI 411 GTAC GT^AC 1026 GTAC Sau3AI 570 GATC ^GATC 801 GATC StuI 920 AGGCCT AGG^CCT 935 AGGCCT XcmI 371 CCACCCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 375 CCAAGGGTTGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI Aor51HI BanI BanII BbvI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI Eco47III EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI HinP1I HphI ItaI MaeIII MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI