################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp509I Tth111I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1180 CGCG CG^CG Alw44I 852 GTGCAC G^TGCAC AlwI 306 GGATC GGATC(4/5) ApaLI 852 GTGCAC G^TGCAC BalI 1391 TGGCCA TGG^CCA BbsI 1195 GTCTTC GAAGAC(2/6) BbvCI 280 GCTGAGG CCTCAGC(-5/-2) BcgI 141 GCACCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 418 CTAG C^TAG BlpI 376 GCTGAGC GC^T[ACGT]AGC Bpu1102I 376 GCTGAGC GC^T[ACGT]AGC BpuAI 1195 GTCTTC GAAGAC(2/6) BsiEI 1216 CGGCCG CG[GA][CT]^CG BsmBI 1424 GAGACG CGTCTC(1/5) BsrDI 546 CATTGC GCAATG(2/0) BsrI 590 CCAGT ACTGG(1/-1) BstAPI 627 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1180 CGCG CG^CG BstYI 306 GGATCT [GA]^GATC[CT] CelII 376 GCTGAGC GC^T[ACGT]AGC CfoI 189 GCGC GCG^C DpnII 307 GATC ^GATC DraIII 754 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1216 CGGCCG C^GGCCG EclXI 1216 CGGCCG C^GGCCG Eco52I 1216 CGGCCG C^GGCCG HaeII 188 GGCGCT [GA]GCGC^[CT] HgaI 120 GACGC GACGC(5/10) HhaI 189 GCGC GCG^C HinP1I 189 GCGC G^CGC HincII 577 GTCAAC GT[CT]^[GA]AC HindII 577 GTCAAC GT[CT]^[GA]AC KspI 1179 CCGCGG CCGC^GG MaeI 418 CTAG C^TAG MaeII 866 ACGT A^CGT MboI 307 GATC ^GATC MflI 306 GGATCT [GA]^GATC[CT] MluNI 1391 TGGCCA TGG^CCA MscI 1391 TGGCCA TGG^CCA MvnI 1180 CGCG CG^CG NaeI 1214 GCCGGC GCC^GGC NdeII 307 GATC ^GATC NgoMIV 1214 GCCGGC G^CCGGC PflMI 1008 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 1412 GAGCTC GAGCT^C SacII 1179 CCGCGG CCGC^GG SapI 340 GAAGAGC GCTCTTC(1/4) Sau3AI 307 GATC ^GATC SspI 255 AATATT AAT^ATT TfiI 470 GATTC G^A[AT]TC Van91I 1008 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 306 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 637 CCTGAGG CC^T[ACGT]AGG 1288 CCTAAGG AspHI 852 GTGCAC G[AT]GC[AT]^C 1412 GAGCTC AvaII 673 GGACC G^G[AT]CC 687 GGACC BglI 1175 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1230 GCCCCCAAGGC BsaI 1099 GAGACC GGTCTC(1/5) 1280 GAGACC BseRI 913 GAGGAG GAGGAG(10/8) 1090 GAGGAG BsgI 139 CTGCAC GTGCAG(16/14) 958 CTGCAC BsiHKAI 852 GTGCAC G[AT]GC[AT]^C 1412 GAGCTC BsrFI 585 ACCGGC [GA]^CCGG[CT] 1214 GCCGGC Bsu36I 637 CCTGAGG CC^T[ACGT]AGG 1288 CCTAAGG Cfr10I 585 ACCGGC [GA]^CCGG[CT] 1214 GCCGGC Eco81I 637 CCTGAGG CC^T[ACGT]AGG 1288 CCTAAGG EcoT14I 1189 CCAAGG C^C[AT][AT]GG 1234 CCAAGG MaeIII 67 GTGAC ^GT[ACGT]AC 868 GTCAC MseI 266 TTAA T^TAA 1244 TTAA PpuMI 672 GGGACCT [GA]G^G[AT]CC[CT] 686 GGGACCC PvuII 15 CAGCTG CAG^CTG 1012 CAGCTG StyI 1189 CCAAGG C^C[AT][AT]GG 1234 CCAAGG Tru9I 266 TTAA T^TAA 1244 TTAA Tsp45I 67 GTGAC ^GT[GC]AC 868 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AlwNI ApaI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PaeR7I PleI PstI Sau96I ScrFI SfaNI SfcI SmaI SmlI TaqI TseI TspRI TthHB8I XhoI XmaCI XmaI