(Circular) (Six-base) MAPSORT of: pFN21AE1461.seq Check: 7290 from: 1 to: 6181 pFN21AE1461 6181 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2157 2157 Size: 6181 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3524 1937 Size: 1587 4594 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 4244 5628 5896 1632 Size: 659 1953 1384 268 1917 Fragments arranged by size: 1953 1917 1384 659 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 5080 6057 1203 Size: 3877 977 1327 Fragments arranged by size: 3877 1327 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3488 1184 Size: 2304 3877 AlwNI CAG_nnn'CTG Cuts at: 2314 2638 3019 3924 5496 2314 Size: 324 381 905 1572 2999 Fragments arranged by size: 2999 1572 905 381 324 ApaI G_GGCC'C Cuts at: 2471 2741 3231 3303 2471 Size: 270 490 72 5349 Fragments arranged by size: 5349 490 270 72 ApaLI G'TGCA_C Cuts at: 2857 4238 5394 2857 Size: 1381 1156 3644 Fragments arranged by size: 3644 1381 1156 ApoI r'AATT_y Cuts at: 1493 3490 3716 3868 5977 1493 Size: 1997 226 152 2109 1697 Fragments arranged by size: 2109 1997 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2280 2397 2673 3049 3132 3230 3313 Size: 73 337 117 276 376 83 98 83 Cuts at: 3313 3426 3506 1870 Size: 113 80 4545 Fragments arranged by size: 4545 376 337 276 117 113 98 83 83 80 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2514 2653 3437 3502 4957 Size: 325 217 627 727 139 784 65 1455 Cuts at: 4957 618 Size: 1842 Fragments arranged by size: 1842 1455 784 727 627 325 217 139 65 BanII G_rGCy'C Cuts at: 1391 2108 2404 2471 2741 2915 3063 3231 3237 Size: 717 296 67 270 174 148 168 6 Cuts at: 3237 3303 3320 3421 3500 1391 Size: 66 17 101 79 4072 Fragments arranged by size: 4072 717 296 270 174 168 148 101 79 67 66 17 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3193 928 Size: 971 1294 3916 Fragments arranged by size: 3916 1294 971 BbvCI CC'TCA_GC Cuts at: 2286 2286 Size: 6181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2135 2169 4372 4406 1903 Size: 34 198 34 2203 34 3678 Fragments arranged by size: 3678 2203 198 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3186 3241 4792 Size: 107 122 71 631 605 1514 55 1551 Cuts at: 4792 136 Size: 1525 Fragments arranged by size: 1551 1525 1514 631 605 122 107 71 55 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 2382 3598 2382 Size: 1216 4965 Bme1580I G_kGCm'C Cuts at: 1790 2471 2741 2861 3231 3303 4242 5398 1790 Size: 681 270 120 370 72 939 1156 2573 Fragments arranged by size: 2573 1156 939 681 370 270 120 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3912 4870 442 Size: 416 53 83 399 24 2495 958 1753 Fragments arranged by size: 2495 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2422 2481 2923 4841 1239 Size: 1183 59 442 1918 2579 Fragments arranged by size: 2579 1918 1183 442 59 Bpu10I CC'TnA_GC Cuts at: 2286 2286 Size: 6181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3098 3279 4844 882 Size: 617 1599 181 1565 2219 Fragments arranged by size: 2219 1599 1565 617 181 BsaAI yAC'GTr Cuts at: 493 1204 3972 493 Size: 711 2768 2702 Fragments arranged by size: 2768 2702 711 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4370 5005 275 Size: 53 83 186 936 2455 382 635 1451 Fragments arranged by size: 2455 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4614 5012 5286 5433 5844 4614 Size: 398 274 147 411 4951 Fragments arranged by size: 4951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 2420 2450 2646 Size: 30 165 30 801 30 186 30 196 Cuts at: 2646 2676 1178 Size: 30 4683 Fragments arranged by size: 4683 801 196 186 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2933 3110 1186 Size: 1747 177 4257 Fragments arranged by size: 4257 1747 177 BseYI C'CCAG_C Cuts at: 2101 2406 2786 2847 3012 3019 3032 3276 5384 Size: 305 380 61 165 7 13 244 2108 Cuts at: 5384 5880 2101 Size: 496 2402 Fragments arranged by size: 2402 2108 496 380 305 244 165 61 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2129 2948 1163 Size: 573 393 819 4396 Fragments arranged by size: 4396 819 573 393 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3224 3631 4392 5420 1540 Size: 275 186 1223 407 761 1028 2301 Fragments arranged by size: 2301 1223 1028 761 407 275 186 BsiHKAI G_wGCw'C Cuts at: 2861 3421 4242 4327 5398 2861 Size: 560 821 85 1071 3644 Fragments arranged by size: 3644 1071 821 560 85 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 BsmBI CGTCTCn'nnnn_ Cuts at: 3423 3423 Size: 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2404 2471 2741 2861 2915 3063 Size: 399 318 296 67 270 120 54 148 Cuts at: 3063 3231 3237 3303 3320 3421 3500 4242 4327 Size: 168 6 66 17 101 79 742 85 Cuts at: 4327 5398 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 318 296 270 168 148 120 101 85 79 67 66 54 17 6 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 2985 3417 3480 3536 844 Size: 1313 828 432 63 56 3489 Fragments arranged by size: 3489 1313 828 432 63 56 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2550 4676 4850 65 Size: 1183 1302 2126 174 1396 Fragments arranged by size: 2126 1396 1302 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2590 3219 4825 5012 1700 Size: 110 780 629 1606 187 2869 Fragments arranged by size: 2869 1606 780 629 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 2638 794 Size: 1844 4337 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2311 3511 3878 4263 4280 5732 5818 5830 Size: 339 1200 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1200 385 367 346 339 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 3294 2643 Size: 651 5530 BtgI C'CryG_G Cuts at: 513 1065 2004 3151 3184 3393 513 Size: 552 939 1147 33 209 3301 Fragments arranged by size: 3301 1147 939 552 209 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 2327 2623 2984 3110 3697 4491 4511 2327 Size: 296 361 126 587 794 20 3997 Fragments arranged by size: 3997 794 587 361 296 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2764 2764 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2592 3221 Size: 54 1425 50 275 18 403 359 629 Cuts at: 3221 3396 3628 4517 6146 8 Size: 175 232 889 1629 43 Fragments arranged by size: 1629 1425 889 629 403 359 275 232 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3221 3628 1537 Size: 275 1409 407 4090 Fragments arranged by size: 4090 1409 407 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2340 2526 3076 4111 1353 Size: 869 118 186 550 1035 3423 Fragments arranged by size: 3423 1035 869 550 186 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3206 4768 5142 5288 387 Size: 1064 138 1617 1562 374 146 1280 Fragments arranged by size: 1617 1562 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3419 3419 Size: 6181 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2422 2481 2923 4244 4841 5628 Size: 393 659 131 59 442 1321 597 787 Cuts at: 5628 5896 1239 Size: 268 1524 Fragments arranged by size: 1524 1321 787 659 597 442 393 268 131 59 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2642 2666 2306 Size: 336 24 5821 Fragments arranged by size: 5821 336 24 EcoO109I rG'GnC_Cy Cuts at: 2467 2678 2692 2776 2827 3227 3299 3300 2467 Size: 211 14 84 51 400 72 1 5348 Fragments arranged by size: 5348 400 211 84 72 51 14 1 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 2197 5328 1678 Size: 519 3131 2531 Fragments arranged by size: 3131 2531 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2734 2766 3032 Size: 32 135 32 980 32 150 32 266 Cuts at: 3032 3064 4822 4854 4896 4928 1373 Size: 32 1758 32 42 32 2626 Fragments arranged by size: 2626 1758 980 266 150 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2584 3525 3771 669 Size: 988 281 646 941 246 3079 Fragments arranged by size: 3079 988 941 646 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 3429 5295 5479 1305 Size: 930 1194 1866 184 2007 Fragments arranged by size: 2007 1866 1194 930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3398 10 Size: 54 1425 343 1566 2793 Fragments arranged by size: 2793 1566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2272 2336 2577 3260 4139 4498 Size: 648 991 115 64 241 683 879 359 Cuts at: 4498 4657 518 Size: 159 2042 Fragments arranged by size: 2042 991 879 683 648 359 241 159 115 64 MspA1I CmG'CkG Cuts at: 1606 2022 2172 2381 3019 3186 3597 4274 5422 Size: 416 150 209 638 167 411 677 1148 Cuts at: 5422 5667 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 638 416 411 245 209 167 150 NaeI GCC'GGC Cuts at: 3221 3221 Size: 6181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4690 Fragments arranged by size: 4690 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NgoMIV G'CCGG_C Cuts at: 3219 3219 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3539 5084 1132 Size: 97 2310 1545 2229 Fragments arranged by size: 2310 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 3019 1108 Size: 1911 4270 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2025 2057 4250 4282 5763 5795 2025 Size: 32 2193 32 1481 32 2411 Fragments arranged by size: 2411 2193 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2678 2692 2678 Size: 14 6167 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2467 2737 3227 3299 2467 Size: 270 490 72 5349 Fragments arranged by size: 5349 490 270 72 PspXI vC'TCGA_Gb Cuts at: 2397 3049 3313 2397 Size: 652 264 5265 Fragments arranged by size: 5265 652 264 PstI C_TGCA'G Cuts at: 830 2021 2634 3117 3414 3533 830 Size: 1191 613 483 297 119 3478 Fragments arranged by size: 3478 1191 613 483 297 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2022 3019 2022 Size: 997 5184 SacI G_AGCT'C Cuts at: 3421 3421 Size: 6181 SacII CC_GC'GG Cuts at: 3187 3187 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SanDI GG'GwC_CC Cuts at: 2692 2692 Size: 6181 SapI GCTCTTCn'nnn_ Cuts at: 2340 2340 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 2017 2630 3113 3410 3529 4664 5345 Size: 219 972 613 483 297 119 1135 681 Cuts at: 5345 5536 826 Size: 191 1471 Fragments arranged by size: 1471 1135 972 681 613 483 297 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 2282 2675 3134 3508 1872 Size: 410 393 459 374 4545 Fragments arranged by size: 4545 459 410 393 374 SmlI C'TyrA_G Cuts at: 820 1017 1943 2397 3049 3313 4285 5186 5448 Size: 197 926 454 652 264 972 901 262 Cuts at: 5448 820 Size: 1553 Fragments arranged by size: 1553 972 926 901 652 454 264 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 2262 4105 5 Size: 47 2210 1843 2081 Fragments arranged by size: 2210 2081 1843 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6181 StyI C'CwwG_G Cuts at: 513 1065 2004 3194 3239 3620 513 Size: 552 939 1190 45 381 3074 Fragments arranged by size: 3074 1190 939 552 381 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2428 2555 3356 4208 4225 4378 4563 Size: 1255 848 127 801 852 17 153 185 Cuts at: 4563 5008 325 Size: 445 1498 Fragments arranged by size: 1498 1255 852 848 801 445 185 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4427 6139 96 Size: 274 80 33 51 1443 2450 1712 138 Fragments arranged by size: 2450 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2646 2678 6141 6173 2646 Size: 32 3463 32 2654 Fragments arranged by size: 3463 2654 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 2397 3049 3313 1943 Size: 454 652 264 4811 Fragments arranged by size: 4811 652 454 264 XmaI C'CCGG_G Cuts at: 1870 2280 2673 3132 3506 1870 Size: 410 393 459 374 4545 Fragments arranged by size: 4545 459 410 393 374 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SexAI SfiI SfoI SrfI SwaI XcmI