(Linear) (Six-base) MAPSORT of: KIEE1461.seq Check: 1735 from: 1 to: 1476 KIEE1461.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 152 1476 Size: 152 1324 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 1476 Size: 286 1190 AlwNI CAG_nnn'CTG Cuts at: 0 309 633 1014 1476 Size: 309 324 381 462 Fragments arranged by size: 462 381 324 309 ApaI G_GGCC'C Cuts at: 0 466 736 1226 1298 1476 Size: 466 270 490 72 178 Fragments arranged by size: 490 466 270 178 72 ApaLI G'TGCA_C Cuts at: 0 852 1476 Size: 852 624 AvaI C'yCGr_G Cuts at: 0 275 392 668 1044 1127 1225 1308 1421 Size: 275 117 276 376 83 98 83 113 Cuts at: 1421 1476 Size: 55 Fragments arranged by size: 376 276 275 117 113 98 83 83 55 BanI G'GyrC_C Cuts at: 0 509 648 1432 1476 Size: 509 139 784 44 Fragments arranged by size: 784 509 139 44 BanII G_rGCy'C Cuts at: 0 103 399 466 736 910 1058 1226 1232 Size: 103 296 67 270 174 148 168 6 Cuts at: 1232 1298 1315 1416 1476 Size: 66 17 101 60 Fragments arranged by size: 296 270 174 168 148 103 101 67 66 60 17 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 1188 1476 Size: 1188 288 BbvCI CC'TCA_GC Cuts at: 0 281 1476 Size: 281 1195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 130 164 1476 Size: 130 34 1312 Fragments arranged by size: 1312 130 34 BglI GCCn_nnn'nGGC Cuts at: 0 1181 1236 1476 Size: 1181 55 240 Fragments arranged by size: 1181 240 55 BlpI GC'TnA_GC Cuts at: 0 377 1476 Size: 377 1099 Bme1580I G_kGCm'C Cuts at: 0 466 736 856 1226 1298 1476 Size: 466 270 120 370 72 178 Fragments arranged by size: 466 370 270 178 120 72 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 117 1476 Size: 117 1359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 417 476 918 1476 Size: 417 59 442 558 Fragments arranged by size: 558 442 417 59 Bpu10I CC'TnA_GC Cuts at: 0 281 1476 Size: 281 1195 BsaI GGTCTCn'nnnn_ Cuts at: 0 1093 1274 1476 Size: 1093 181 202 Fragments arranged by size: 1093 202 181 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 415 445 641 671 1476 Size: 199 30 186 30 196 30 805 Fragments arranged by size: 805 199 196 186 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 928 1105 1476 Size: 928 177 371 Fragments arranged by size: 928 371 177 BseYI C'CCAG_C Cuts at: 0 96 401 781 842 1007 1014 1027 1271 Size: 96 305 380 61 165 7 13 244 Cuts at: 1271 1476 Size: 205 Fragments arranged by size: 380 305 244 205 165 96 61 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 124 943 1476 Size: 124 819 533 Fragments arranged by size: 819 533 124 BsiEI CG_ry'CG Cuts at: 0 1219 1476 Size: 1219 257 BsiHKAI G_wGCw'C Cuts at: 0 856 1416 1476 Size: 856 560 60 Fragments arranged by size: 856 560 60 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1418 1476 Size: 1418 58 Bsp1286I G_dGCh'C Cuts at: 0 103 399 466 736 856 910 1058 1226 Size: 103 296 67 270 120 54 148 168 Cuts at: 1226 1232 1298 1315 1416 1476 Size: 6 66 17 101 60 Fragments arranged by size: 296 270 168 148 120 103 101 67 66 60 54 17 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 152 980 1412 1475 1476 Size: 152 828 432 63 1 Fragments arranged by size: 828 432 152 63 1 BsrDI GCAATG_nn' Cuts at: 0 545 1476 Size: 545 931 BsrFI r'CCGG_y Cuts at: 0 585 1214 1476 Size: 585 629 262 Fragments arranged by size: 629 585 262 BstAPI GCAn_nnn'nTGC Cuts at: 0 633 1476 Size: 633 843 BstYI r'GATC_y Cuts at: 0 306 1476 Size: 306 1170 Bsu36I CC'TnA_GG Cuts at: 0 638 1289 1476 Size: 638 651 187 Fragments arranged by size: 651 638 187 BtgI C'CryG_G Cuts at: 0 1146 1179 1388 1476 Size: 1146 33 209 88 Fragments arranged by size: 1146 209 88 33 BtsI GCAGTG_nn' Cuts at: 0 322 618 979 1105 1476 Size: 322 296 361 126 371 Fragments arranged by size: 371 361 322 296 126 DraIII CAC_nnn'GTG Cuts at: 0 759 1476 Size: 759 717 EaeI y'GGCC_r Cuts at: 0 228 587 1216 1391 1476 Size: 228 359 629 175 85 Fragments arranged by size: 629 359 228 175 85 EagI C'GGCC_G Cuts at: 0 1216 1476 Size: 1216 260 EarI CTCTTCn'nnn_ Cuts at: 0 217 335 521 1071 1476 Size: 217 118 186 550 405 Fragments arranged by size: 550 405 217 186 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1201 1476 Size: 1201 275 EcoICRI GAG'CTC Cuts at: 0 1414 1476 Size: 1414 62 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 417 476 918 1476 Size: 286 131 59 442 558 Fragments arranged by size: 558 442 286 131 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 301 637 661 1476 Size: 301 336 24 815 Fragments arranged by size: 815 336 301 24 EcoO109I rG'GnC_Cy Cuts at: 0 462 673 687 771 822 1222 1294 1295 Size: 462 211 14 84 51 400 72 1 Cuts at: 1295 1476 Size: 181 Fragments arranged by size: 462 400 211 181 84 72 51 14 1 HaeII r_GCGC'y Cuts at: 0 192 1476 Size: 192 1284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 547 579 729 761 1027 1059 1476 Size: 547 32 150 32 266 32 417 Fragments arranged by size: 547 417 266 150 32 32 32 HincII GTy'rAC Cuts at: 0 579 1476 Size: 579 897 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 230 1424 1476 Size: 230 1194 52 Fragments arranged by size: 1194 230 52 MscI TGG'CCA Cuts at: 0 1393 1476 Size: 1393 83 MslI CAynn'nnrTG Cuts at: 0 152 267 331 572 1255 1476 Size: 152 115 64 241 683 221 Fragments arranged by size: 683 241 221 152 115 64 MspA1I CmG'CkG Cuts at: 0 17 167 376 1014 1181 1476 Size: 17 150 209 638 167 295 Fragments arranged by size: 638 295 209 167 150 17 NaeI GCC'GGC Cuts at: 0 1216 1476 Size: 1216 260 NgoMIV G'CCGG_C Cuts at: 0 1214 1476 Size: 1214 262 PflMI CCAn_nnn'nTGG Cuts at: 0 1014 1476 Size: 1014 462 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 20 52 1476 Size: 20 32 1424 Fragments arranged by size: 1424 32 20 PpuMI rG'GwC_Cy Cuts at: 0 673 687 1476 Size: 673 14 789 Fragments arranged by size: 789 673 14 PspOMI G'GGCC_C Cuts at: 0 462 732 1222 1294 1476 Size: 462 270 490 72 182 Fragments arranged by size: 490 462 270 182 72 PspXI vC'TCGA_Gb Cuts at: 0 392 1044 1308 1476 Size: 392 652 264 168 Fragments arranged by size: 652 392 264 168 PstI C_TGCA'G Cuts at: 0 16 629 1112 1409 1476 Size: 16 613 483 297 67 Fragments arranged by size: 613 483 297 67 16 PvuII CAG'CTG Cuts at: 0 17 1014 1476 Size: 17 997 462 Fragments arranged by size: 997 462 17 SacI G_AGCT'C Cuts at: 0 1416 1476 Size: 1416 60 SacII CC_GC'GG Cuts at: 0 1182 1476 Size: 1182 294 SanDI GG'GwC_CC Cuts at: 0 687 1476 Size: 687 789 SapI GCTCTTCn'nnn_ Cuts at: 0 335 1476 Size: 335 1141 SfcI C'TryA_G Cuts at: 0 12 625 1108 1405 1476 Size: 12 613 483 297 71 Fragments arranged by size: 613 483 297 71 12 SmaI CCC'GGG Cuts at: 0 277 670 1129 1476 Size: 277 393 459 347 Fragments arranged by size: 459 393 347 277 SmlI C'TyrA_G Cuts at: 0 392 1044 1308 1476 Size: 392 652 264 168 Fragments arranged by size: 652 392 264 168 SspI AAT'ATT Cuts at: 0 257 1476 Size: 257 1219 StyI C'CwwG_G Cuts at: 0 1189 1234 1476 Size: 1189 45 242 Fragments arranged by size: 1189 242 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 423 550 1351 1476 Size: 423 127 801 125 Fragments arranged by size: 801 423 127 125 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 641 673 1476 Size: 641 32 803 Fragments arranged by size: 803 641 32 XhoI C'TCGA_G Cuts at: 0 392 1044 1308 1476 Size: 392 652 264 168 Fragments arranged by size: 652 392 264 168 XmaI C'CCGG_G Cuts at: 0 275 668 1127 1476 Size: 275 393 459 349 Fragments arranged by size: 459 393 349 275 Enzymes that do cut: AarI AcuI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV PflMI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SacII SanDI SapI SfcI SmaI SmlI SspI StyI TaqII TstI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XcmI XmnI ZraI