Restriction map for
pFN21AE4322
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstF5I BstZ17I Bsu36I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HpaI KasI KpnI
MamI MluI MroI MseI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tru9I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 844 GTCTAC GT^[AC][GT]AC
AccII 26 CGCG CG^CG
AcsI 324 AAATTT [GA]^AATT[CT]
AcyI 45 GGCGTC G[GA]^CG[CT]C
AfaI 498 GTAC GT^AC
AhdI 646 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 635 GGATC GGATC(4/5)
ApoI 324 AAATTT [GA]^AATT[CT]
Asp700I 330 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 646 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 119 CCCGAG C^[CT]CG[GA]G
AvaII 115 GGTCC G^G[AT]CC
BanI 625 GGCACC G^G[CT][GA]CC
BanII 394 GAGCTC G[GA]GC[CT]^C
BbsI 835 GAAGAC GAAGAC(2/6)
BbvCI 556 GCTGAGG CCTCAGC(-5/-2)
BcnI 54 CCGGG CC^[GC]GG
BpuAI 835 GAAGAC GAAGAC(2/6)
BsaHI 45 GGCGTC G[GA]^CG[CT]C
BseRI 430 GAGGAG GAGGAG(10/8)
BsmBI 501 CGTCTC CGTCTC(1/5)
BsoBI 119 CCCGAG C^[CT]CG[GA]G
BspHI 638 TCATGA T^CATGA
BsrDI 7 GCAATG GCAATG(2/0)
BstUI 26 CGCG CG^CG
BstXI 614 CCATTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 25 CCGCGG C^C[GA][CT]GG
Eam1105I 646 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 523 CCTTATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 362 CCAAGG C^C[AT][AT]GG
HaeII 18 GGCGCT [GA]GCGC^[CT]
HapII 54 CCGG C^CGG
Hin1I 45 GGCGTC G[GA]^CG[CT]C
HindIII 892 AAGCTT A^AGCTT
HpaII 54 CCGG C^CGG
KspI 25 CCGCGG CCGC^GG
MaeII 500 ACGT A^CGT
MfeI 417 CAATTG C^AATTG
MspI 54 CCGG C^CGG
MunI 417 CAATTG C^AATTG
MvnI 26 CGCG CG^CG
NciI 54 CCGGG CC^[GC]GG
PflFI 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 745 CTGCAG CTGCA^G
RcaI 638 TCATGA T^CATGA
RsaI 498 GTAC GT^AC
SacI 394 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SapI 396 GCTCTTC GCTCTTC(1/4)
SexAI 839 ACCTGGT A^CC[AT]GGT
SfcI 745 CTGCAG C^T[GA][CT]AG
SspI 481 AATATT AAT^ATT
StyI 362 CCAAGG C^C[AT][AT]GG
TaqI 338 TCGA T^CGA
TfiI 882 GAATC G^A[AT]TC
Tth111I 838 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 338 TCGA T^CGA
XmnI 330 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AspHI 394 GAGCTC G[AT]GC[AT]^C
509 GTGCTC
BalI 725 TGGCCA TGG^CCA
827 TGGCCA
BfaI 853 CTAG C^TAG
941 CTAG
BglII 536 AGATCT A^GATCT
596 AGATCT
BsiHKAI 394 GAGCTC G[AT]GC[AT]^C
509 GTGCTC
BsmAI 231 GTCTC GTCTC(1/5)
502 GTCTC
BsmI 281 GCATTC GAATGC(1/-1)
866 GCATTC
BstAPI 343 GCAGCGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
666 GCAGGAAGTGC
BstEII 256 GGTGACC G^GT[ACGT]ACC
561 GGTCACC
BstPI 256 GGTGACC G^GT[ACGT]ACC
561 GGTCACC
BstYI 536 AGATCT [GA]^GATC[CT]
596 AGATCT
CfoI 19 GCGC GCG^C
469 GCGC
Cfr13I 115 GGTCC G^G[ACGT]CC
741 GGCCC
EaeI 725 TGGCCA [CT]^GGCC[GA]
827 TGGCCA
EarI 397 CTCTTC CTCTTC(1/4)
771 CTCTTC
EcoO65I 256 GGTGACC G^GT[ACGT]ACC
561 GGTCACC
FauI 93 GCGGG CCCGC(4/6)
492 GCGGG
HgaI 451 GACGC GACGC(5/10)
46 GCGTC
HhaI 19 GCGC GCG^C
469 GCGC
HinP1I 19 GCGC G^CGC
469 GCGC
Ksp632I 397 CTCTTC CTCTTC(1/4)
771 CTCTTC
MaeI 853 CTAG C^TAG
941 CTAG
MaeIII 257 GTGAC ^GT[ACGT]AC
562 GTCAC
MflI 536 AGATCT [GA]^GATC[CT]
596 AGATCT
MluNI 725 TGGCCA TGG^CCA
827 TGGCCA
MscI 725 TGGCCA TGG^CCA
827 TGGCCA
MspA1I 25 CCGCGG C[AC]G^C[GT]G
344 CAGCGG
PleI 200 GACTC GAGTC(4/5)
769 GACTC
Sau96I 115 GGTCC G^G[ACGT]CC
741 GGCCC
SfaNI 299 GCATC GCATC(5/9)
814 GCATC
Tsp45I 257 GTGAC ^GT[GC]AC
562 GTCAC
XhoII 536 AGATCT [GA]^GATC[CT]
596 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI DdeI DpnII
EcoRII Fnu4HI HaeIII HinfI HphI
ItaI MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI Sau3AI ScrFI TseI Tsp509I
TspRI XcmI