Restriction map for
pFN21AE4322
(Circular) (Six-base) MAPSORT of: pFN21AE4322.seq Check: 4822 from: 1 to: 5680
pFN21AE4322 5680 bp
With 182 enzymes: SgfI *
March 28, 2012 14:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3490 278
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 3023 1937
Size: 913 173 4594
Fragments arranged by size:
4594 913 173
Acc65I G'GTAC_C
Cuts at: 3001 3001
Size: 5680
AclI AA'CG_TT
Cuts at: 1563 3807 4180 1563
Size: 2244 373 3063
Fragments arranged by size:
3063 2244 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2292 2486 3743 5127 5395 1632
Size: 585 75 194 1257 1384 268 1917
Fragments arranged by size:
1917 1384 1257 585 268 194 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5483
AflIII A'CryG_T
Cuts at: 1203 4579 5556 1203
Size: 3376 977 1327
Fragments arranged by size:
3376 1327 977
AgeI A'CCGG_T
Cuts at: 4511 4511
Size: 5680
AhdI GACnn_n'nnGTC
Cuts at: 2656 4409 2656
Size: 1753 3927
AleI CACnn'nnGTG
Cuts at: 1166 2880 2882 1166
Size: 1714 2 3964
Fragments arranged by size:
3964 1714 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5680
AlwNI CAG_nnn'CTG
Cuts at: 3423 4995 3423
Size: 1572 4108
ApaLI G'TGCA_C
Cuts at: 3737 4893 3737
Size: 1156 4524
ApoI r'AATT_y
Cuts at: 1493 2329 2989 3215 3367 5476 1493
Size: 836 660 226 152 2109 1697
Fragments arranged by size:
2109 1697 836 660 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4234 160
Size: 872 3202 1606
Fragments arranged by size:
3202 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 3005 1870
Size: 73 181 881 4545
Fragments arranged by size:
4545 881 181 73
BamHI G'GATC_C
Cuts at: 3010 3377 3010
Size: 367 5313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 3001 4456 618
Size: 325 217 627 843 371 1455 1842
Fragments arranged by size:
1842 1455 843 627 371 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2999 1391
Size: 1012 596 4072
Fragments arranged by size:
4072 1012 596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2847 928
Size: 971 948 3761
Fragments arranged by size:
3761 971 948
BbvCI CC'TCA_GC
Cuts at: 2562 2562
Size: 5680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3871 3905 1903
Size: 34 1934 34 3678
Fragments arranged by size:
3678 1934 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3572 4782 3572
Size: 1210 4470
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5680
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4291 136
Size: 107 122 71 631 605 2619 1525
Fragments arranged by size:
2619 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2541 2601 5675 2541
Size: 60 3074 2546
Fragments arranged by size:
3074 2546 60
BlpI GC'TnA_GC
Cuts at: 3097 3097
Size: 5680
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2633 3741 4897 1790
Size: 297 546 1108 1156 2573
Fragments arranged by size:
2573 1156 1108 546 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5680
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 3411 4369
Size: 416 53 83 399 24 892 1102 958
Cuts at: 4369 442
Size: 1753
Fragments arranged by size:
1753 1102 958 892 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 2382 2876 4340 1239
Size: 909 234 494 1464 2579
Fragments arranged by size:
2579 1464 909 494 234
Bpu10I CC'TnA_GC
Cuts at: 2562 2961 2562
Size: 399 5281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3805 4670 4968 3805
Size: 865 298 4517
Fragments arranged by size:
4517 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4343 882
Size: 617 2844 2219
Fragments arranged by size:
2844 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3471 493
Size: 711 2267 2702
Fragments arranged by size:
2702 2267 711
BsaBI GATnn'nnATC
Cuts at: 5674 5674
Size: 5680
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2051 3487 3869 4504
Size: 53 83 186 936 518 1436 382 635
Cuts at: 4504 275
Size: 1451
Fragments arranged by size:
1451 1436 936 635 518 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4113 4511 4785 4932 5343 4113
Size: 398 274 147 411 4450
Fragments arranged by size:
4450 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 2751 2781 1178
Size: 30 165 30 1185 30 133 30 4077
Fragments arranged by size:
4077 1185 165 133 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2450 1186
Size: 1264 4416
BseYI C'CCAG_C
Cuts at: 2548 4883 5379 2548
Size: 2335 496 2849
Fragments arranged by size:
2849 2335 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3130 3891 4919 1540
Size: 275 186 1129 761 1028 2301
Fragments arranged by size:
2301 1129 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2518 3741 3826 4897 2403
Size: 115 1223 85 1071 3186
Fragments arranged by size:
3186 1223 1071 115 85
BsmI GAATG_Cn'
Cuts at: 2286 2871 3191 3284 2286
Size: 585 320 93 4682
Fragments arranged by size:
4682 585 320 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2512 2512
Size: 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2403 2518 2633 2999 3741 3826
Size: 399 297 316 115 115 366 742 85
Cuts at: 3826 4897 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 316 297 115
115 85
BspEI T'CCGG_A
Cuts at: 5343 5343
Size: 5680
BspHI T'CATG_A
Cuts at: 2643 3569 5299 2643
Size: 926 1730 3024
Fragments arranged by size:
3024 1730 926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3035 844
Size: 2191 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3567 1394
Size: 429 1744 3507
Fragments arranged by size:
3507 1744 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2019 4175 4349 65
Size: 1183 771 2156 174 1396
Fragments arranged by size:
2156 1396 1183 771 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4324 4511 1700
Size: 110 2514 187 2869
Fragments arranged by size:
2869 2514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5680
BssSI C'ACGA_G
Cuts at: 3740 4752 3740
Size: 1012 4668
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2354 2677 794
Size: 1560 323 3797
Fragments arranged by size:
3797 1560 323
BstBI TT'CG_AA
Cuts at: 4484 4484
Size: 5680
BstEII G'GTnAC_C
Cuts at: 1184 2261 2566 1184
Size: 1077 305 4298
Fragments arranged by size:
4298 1077 305
BstXI CCAn_nnnn'nTGG
Cuts at: 2626 2626
Size: 5680
BstYI r'GATC_y
Cuts at: 1972 2541 2601 3010 3377 3762 3779 5231 5317
Size: 569 60 409 367 385 17 1452 86
Cuts at: 5317 5329 5675 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 569 409 385 367 346 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 513
Size: 552 939 26 4163
Fragments arranged by size:
4163 939 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4941
Fragments arranged by size:
4941 683 56
BtsI GCAGTG_nn'
Cuts at: 2701 3196 3990 4010 2701
Size: 495 794 20 4371
Fragments arranged by size:
4371 794 495 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5680
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2212 2247 529
Size: 35 1648 35 3962
Fragments arranged by size:
3962 1648 35 35
DraI TTT'AAA
Cuts at: 2984 2984
Size: 5680
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4687 809
Size: 507 3371 1802
Fragments arranged by size:
3371 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2730 2832 3127
Size: 54 1425 50 275 18 900 102 295
Cuts at: 3127 4016 5645 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 900 889 295 275 102 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3127 1537
Size: 275 1315 4090
Fragments arranged by size:
4090 1315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2408 2782 3610 1353
Size: 1055 374 828 3423
Fragments arranged by size:
3423 1055 828 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4267 4641 4787 387
Size: 1064 138 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2217 2292 2382 2486 2876 3743
Size: 393 516 69 75 90 104 390 867
Cuts at: 3743 4340 5127 5395 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 867 787 597 516 393 390 268
104 90 75 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2532 2532
Size: 5680
EcoRI G'AATT_C
Cuts at: 2989 3367 2989
Size: 378 5302
FspI TGC'GCA
Cuts at: 4186 4186
Size: 5680
HaeII r_GCGC'y
Cuts at: 1678 2027 4827 1678
Size: 349 2800 2531
Fragments arranged by size:
2800 2531 349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 2751 2783 4321
Size: 32 135 32 1070 32 77 32 1538
Cuts at: 4321 4353 4395 4427 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1538 1070 135 77 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3024 3270 669
Size: 988 281 1086 246 3079
Fragments arranged by size:
3079 1086 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2897 748
Size: 2149 3531
HpaI GTT'AAC
Cuts at: 3270 3270
Size: 5680
KpnI G_GTAC'C
Cuts at: 3005 3005
Size: 5680
MfeI C'AATT_G
Cuts at: 2422 3279 2422
Size: 857 4823
MluI A'CGCG_T
Cuts at: 5556 5556
Size: 5680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4794 4978 1305
Size: 3489 184 2007
Fragments arranged by size:
3489 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2732 2834 10
Size: 54 1425 343 900 102 2856
Fragments arranged by size:
2856 1425 900 343 102 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2624 2706 2741 2880 2882 3638 3997
Size: 648 1458 82 35 139 2 756 359
Cuts at: 3997 4156 518
Size: 159 2042
Fragments arranged by size:
2042 1458 756 648 359 159 139 82
35 2
MspA1I CmG'CkG
Cuts at: 1606 2032 2351 3096 3773 4921 5166 1606
Size: 426 319 745 677 1148 245 2120
Fragments arranged by size:
2120 1148 745 677 426 319 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4189
Fragments arranged by size:
4189 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5680
NotI GC'GGCC_GC
Cuts at: 3127 3127
Size: 5680
NspI r_CATG'y
Cuts at: 1132 1229 2191 2656 2857 3038 4583 1132
Size: 97 962 465 201 181 1545 2229
Fragments arranged by size:
2229 1545 962 465 201 181 97
PasI CC'CwG_GG
Cuts at: 1348 2608 2636 1348
Size: 1260 28 4392
Fragments arranged by size:
4392 1260 28
PciI A'CATG_T
Cuts at: 4579 4579
Size: 5680
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5680
PmeI GTTT'AAAC
Cuts at: 2984 2984
Size: 5680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 5262 5294 3749
Size: 32 1481 32 4135
Fragments arranged by size:
4135 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5680
PsiI TTA'TAA
Cuts at: 3250 3250
Size: 5680
PstI C_TGCA'G
Cuts at: 830 2754 3032 830
Size: 1924 278 3478
Fragments arranged by size:
3478 1924 278
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5680
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5680
SacII CC_GC'GG
Cuts at: 2033 2033
Size: 5680
SalI G'TCGA_C
Cuts at: 1936 3022 1936
Size: 1086 4594
SapI GCTCTTCn'nnn_
Cuts at: 2408 2408
Size: 5680
SbfI CC_TGCA'GG
Cuts at: 3032 3032
Size: 5680
ScaI AGT'ACT
Cuts at: 3928 5640 3928
Size: 1712 3968
SexAI A'CCwGG_T
Cuts at: 2844 2844
Size: 5680
SfcI C'TryA_G
Cuts at: 826 1045 2750 3028 4163 4844 5035 826
Size: 219 1705 278 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 681 278 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5680
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5680
SmaI CCC'GGG
Cuts at: 1872 3007 1872
Size: 1135 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3784 4685 4947 820
Size: 197 926 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3471 493
Size: 2978 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5680
SphI G_CATG'C
Cuts at: 1132 3038 1132
Size: 1906 3774
SspI AAT'ATT
Cuts at: 5 52 2488 3604 5
Size: 47 2436 1116 2081
Fragments arranged by size:
2436 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5680
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 3119 513
Size: 552 939 363 752 3074
Fragments arranged by size:
3074 939 752 552 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3707 3724 3877 4062 4507 325
Size: 1255 669 1458 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 669 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3926 5638 96
Size: 274 80 33 51 1443 1949 1712 138
Fragments arranged by size:
1949 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5640 5672 5640
Size: 32 5648
Tth111I GACn'n_nGTC
Cuts at: 1316 2846 1316
Size: 1530 4150
XbaI T'CTAG_A
Cuts at: 3016 3016
Size: 5680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2714 2740 2842 2714
Size: 26 102 5552
Fragments arranged by size:
5552 102 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5680
XmaI C'CCGG_G
Cuts at: 1870 3005 1870
Size: 1135 4545
XmnI GAAnn'nnTTC
Cuts at: 2339 3809 2339
Size: 1470 4210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3488 276
Size: 53 83 186 936 1954 2468
Fragments arranged by size:
2468 1954 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SanDI SfiI SfoI SrfI SwaI