(Linear) (Six-base) MAPSORT of: KIEE4322.seq Check: 7288 from: 1 to: 975 KIEE4322.orf With 182 enzymes: SgfI * March 22, 2012 17:43 .. AccI GT'mk_AC Cuts at: 0 845 975 Size: 845 130 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 287 481 975 Size: 212 75 194 494 Fragments arranged by size: 494 212 194 75 AhdI GACnn_n'nnGTC Cuts at: 0 651 975 Size: 651 324 AleI CACnn'nnGTG Cuts at: 0 875 877 975 Size: 875 2 98 Fragments arranged by size: 875 98 2 ApoI r'AATT_y Cuts at: 0 324 975 Size: 324 651 AvaI C'yCGr_G Cuts at: 0 119 975 Size: 119 856 BanI G'GyrC_C Cuts at: 0 625 975 Size: 625 350 BanII G_rGCy'C Cuts at: 0 398 975 Size: 398 577 BbsI GAAGACnn'nnnn_ Cuts at: 0 842 975 Size: 842 133 BbvCI CC'TCA_GC Cuts at: 0 557 975 Size: 557 418 BglII A'GATC_T Cuts at: 0 536 596 975 Size: 536 60 379 Fragments arranged by size: 536 379 60 Bme1580I G_kGCm'C Cuts at: 0 82 628 975 Size: 82 546 347 Fragments arranged by size: 546 347 82 BmrI ACTGGGnnnn_n' Cuts at: 0 304 975 Size: 304 671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 143 377 871 975 Size: 143 234 494 104 Fragments arranged by size: 494 234 143 104 Bpu10I CC'TnA_GC Cuts at: 0 557 956 975 Size: 557 399 19 Fragments arranged by size: 557 399 19 BsaHI Gr'CG_yC Cuts at: 0 46 975 Size: 46 929 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 583 613 746 776 975 Size: 583 30 133 30 199 Fragments arranged by size: 583 199 133 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 445 975 Size: 445 530 BseYI C'CCAG_C Cuts at: 0 543 975 Size: 543 432 BsiHKAI G_wGCw'C Cuts at: 0 398 513 975 Size: 398 115 462 Fragments arranged by size: 462 398 115 BsmI GAATG_Cn' Cuts at: 0 281 866 975 Size: 281 585 109 Fragments arranged by size: 585 281 109 BsmBI CGTCTCn'nnnn_ Cuts at: 0 507 975 Size: 507 468 Bsp1286I G_dGCh'C Cuts at: 0 82 398 513 628 975 Size: 82 316 115 115 347 Fragments arranged by size: 347 316 115 115 82 BspHI T'CATG_A Cuts at: 0 638 975 Size: 638 337 BsrDI GCAATG_nn' Cuts at: 0 14 975 Size: 14 961 BstAPI GCAn_nnn'nTGC Cuts at: 0 349 672 975 Size: 349 323 303 Fragments arranged by size: 349 323 303 BstEII G'GTnAC_C Cuts at: 0 256 561 975 Size: 256 305 414 Fragments arranged by size: 414 305 256 BstXI CCAn_nnnn'nTGG Cuts at: 0 621 975 Size: 621 354 BstYI r'GATC_y Cuts at: 0 536 596 975 Size: 536 60 379 Fragments arranged by size: 536 379 60 BtgI C'CryG_G Cuts at: 0 25 975 Size: 25 950 BtsI GCAGTG_nn' Cuts at: 0 696 975 Size: 696 279 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 207 242 975 Size: 207 35 733 Fragments arranged by size: 733 207 35 EaeI y'GGCC_r Cuts at: 0 725 827 975 Size: 725 102 148 Fragments arranged by size: 725 148 102 EarI CTCTTCn'nnn_ Cuts at: 0 403 777 975 Size: 403 374 198 Fragments arranged by size: 403 374 198 EcoICRI GAG'CTC Cuts at: 0 396 975 Size: 396 579 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 143 212 287 377 481 871 975 Size: 143 69 75 90 104 390 104 Fragments arranged by size: 390 143 104 104 90 75 69 EcoNI CCTnn'n_nnAGG Cuts at: 0 527 975 Size: 527 448 HaeII r_GCGC'y Cuts at: 0 22 975 Size: 22 953 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 637 669 746 778 975 Size: 637 32 77 32 197 Fragments arranged by size: 637 197 77 32 32 HindIII A'AGCT_T Cuts at: 0 892 975 Size: 892 83 MfeI C'AATT_G Cuts at: 0 417 975 Size: 417 558 MscI TGG'CCA Cuts at: 0 727 829 975 Size: 727 102 146 Fragments arranged by size: 727 146 102 MslI CAynn'nnrTG Cuts at: 0 619 701 736 875 877 975 Size: 619 82 35 139 2 98 Fragments arranged by size: 619 139 98 82 35 2 MspA1I CmG'CkG Cuts at: 0 27 346 975 Size: 27 319 629 Fragments arranged by size: 629 319 27 NspI r_CATG'y Cuts at: 0 186 651 852 975 Size: 186 465 201 123 Fragments arranged by size: 465 201 186 123 PasI CC'CwG_GG Cuts at: 0 603 631 975 Size: 603 28 344 Fragments arranged by size: 603 344 28 PstI C_TGCA'G Cuts at: 0 749 975 Size: 749 226 SacI G_AGCT'C Cuts at: 0 398 975 Size: 398 577 SacII CC_GC'GG Cuts at: 0 28 975 Size: 28 947 SapI GCTCTTCn'nnn_ Cuts at: 0 403 975 Size: 403 572 SexAI A'CCwGG_T Cuts at: 0 839 975 Size: 839 136 SfcI C'TryA_G Cuts at: 0 745 975 Size: 745 230 SspI AAT'ATT Cuts at: 0 483 975 Size: 483 492 StyI C'CwwG_G Cuts at: 0 362 975 Size: 362 613 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 244 975 Size: 244 731 Tth111I GACn'n_nGTC Cuts at: 0 841 975 Size: 841 134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 709 735 837 975 Size: 709 26 102 138 Fragments arranged by size: 709 138 102 26 XmnI GAAnn'nnTTC Cuts at: 0 334 975 Size: 334 641 Enzymes that do cut: AccI AcuI AhdI AleI ApoI AvaI BanI BanII BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI HaeII Hin4I HindIII MfeI MscI MslI MspA1I NspI PasI PstI SacI SacII SapI SexAI SfcI SspI StyI TaqII Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI XbaI XhoI XmaI ZraI