Restriction map for
pFN21AB6642
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I EcoNI EcoRV
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1102 GTCTAC GT^[AC][GT]AC
AcsI 1260 GAATTC [GA]^AATT[CT]
AflIII 1076 ACGTGT A^C[GA][CT]GT
Aor51HI 644 AGCGCT AGC^GCT
ApoI 1260 GAATTC [GA]^AATT[CT]
AviII 870 TGCGCA TGC^GCA
BalI 827 TGGCCA TGG^CCA
BanI 1111 GGTGCC G^G[CT][GA]CC
BcgI 393 GCACGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1231 TACGTG [CT]AC^GT[GA]
BseRI 77 CTCCTC GAGGAG(10/8)
BsgI 391 CTGCAC GTGCAG(16/14)
BsiEI 1207 CGGCCG CG[GA][CT]^CG
BsmI 112 GAATGC GAATGC(1/-1)
BspHI 1268 TCATGA T^CATGA
BsrFI 1209 GCCGGT [GA]^CCGG[CT]
Cfr10I 1209 GCCGGT [GA]^CCGG[CT]
DsaI 909 CCATGG C^C[GA][CT]GG
EagI 1207 CGGCCG C^GGCCG
EarI 91 GAAGAG CTCTTC(1/4)
EclXI 1207 CGGCCG C^GGCCG
Eco47III 644 AGCGCT AGC^GCT
Eco52I 1207 CGGCCG C^GGCCG
EcoRI 1260 GAATTC G^AATTC
EcoT14I 909 CCATGG C^C[AT][AT]GG
FspI 870 TGCGCA TGC^GCA
HgaI 773 GCGTC GACGC(5/10)
Ksp632I 91 GAAGAG CTCTTC(1/4)
MboII 91 GAAGA GAAGA(8/7)
MluNI 827 TGGCCA TGG^CCA
MscI 827 TGGCCA TGG^CCA
MslI 703 CATCGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 909 CCATGG C^CATGG
PflMI 819 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 862 GAGTC GAGTC(4/5)
PshAI 1095 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 720 CTGCAG CTGCA^G
RcaI 1268 TCATGA T^CATGA
SapI 91 GAAGAGC GCTCTTC(1/4)
SfcI 720 CTGCAG C^T[GA][CT]AG
StuI 188 AGGCCT AGG^CCT
StyI 909 CCATGG C^C[AT][AT]GG
TaqI 1008 TCGA T^CGA
TfiI 85 GATTC G^A[AT]TC
Tsp509I 1261 AATT ^AATT
TthHB8I 1008 TCGA T^CGA
Van91I 819 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 444 GTGCAC G^TGCAC
1030 GTGCAC
AlwNI 385 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
820 CAGCTGCTG
AocI 79 CCTCAGG CC^T[ACGT]AGG
184 CCTGAGG
ApaLI 444 GTGCAC G^TGCAC
1030 GTGCAC
BglI 326 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
683 GCCACCAGGGC
BpmI 747 CTCCAG CTGGAG(16/14)
1243 CTGGAG
BsaI 29 GGTCTC GGTCTC(1/5)
963 GGTCTC
BspMI 448 ACCTGC ACCTGC(4/8)
536 ACCTGC
BstYI 1085 AGATCC [GA]^GATC[CT]
1130 AGATCC
Bsu36I 79 CCTCAGG CC^T[ACGT]AGG
184 CCTGAGG
EaeI 827 TGGCCA [CT]^GGCC[GA]
1207 CGGCCG
Eco81I 79 CCTCAGG CC^T[ACGT]AGG
184 CCTGAGG
HinfI 85 GATTC G^A[ACGT]TC
862 GAGTC
MflI 1085 AGATCC [GA]^GATC[CT]
1130 AGATCC
SfaNI 212 GCATC GCATC(5/9)
702 GCATC
SmaI 25 CCCGGG CCC^GGG
1223 CCCGGG
XcmI 420 CCATAACTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
935 CCAACCGCTGCGTGG
XhoII 1085 AGATCC [GA]^GATC[CT]
1130 AGATCC
XmaCI 25 CCCGGG C^CCGGG
1223 CCCGGG
XmaI 25 CCCGGG C^CCGGG
1223 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstEII BstF5I
BstNI BstPI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoO65I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HpaII HphI ItaI MaeII
MaeIII MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI TseI Tsp45I TspRI