(Circular) (Six-base) MAPSORT of: pFN21AB6642.seq Check: 1853 from: 1 to: 6034 pFN21AB6642 6034 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2462 2462 Size: 6034 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3108 3377 1937 Size: 1171 269 4594 Fragments arranged by size: 4594 1171 269 Acc65I G'GTAC_C Cuts at: 3355 3355 Size: 6034 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 4097 5481 5749 1632 Size: 483 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 483 268 AfeI AGC'GCT Cuts at: 2651 2651 Size: 6034 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 3081 4933 5910 1203 Size: 1878 1852 977 1327 Fragments arranged by size: 1878 1852 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6034 AlwNI CAG_nnn'CTG Cuts at: 2395 2830 3777 5349 2395 Size: 435 947 1572 3080 Fragments arranged by size: 3080 1572 947 435 ApaI G_GGCC'C Cuts at: 2484 2638 3227 2484 Size: 154 589 5291 Fragments arranged by size: 5291 589 154 ApaLI G'TGCA_C Cuts at: 2449 3035 4091 5247 2449 Size: 586 1056 1156 3236 Fragments arranged by size: 3236 1156 1056 586 ApoI r'AATT_y Cuts at: 1493 3265 3343 3569 3721 5830 1493 Size: 1772 78 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 78 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 2030 2483 3228 3359 1870 Size: 73 87 453 745 131 4545 Fragments arranged by size: 4545 745 453 131 87 73 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3116 3355 4810 618 Size: 325 217 627 1329 239 1455 1842 Fragments arranged by size: 1842 1455 1329 627 325 239 217 BanII G_rGCy'C Cuts at: 1391 2103 2349 2484 2638 2958 3227 3353 1391 Size: 712 246 135 154 320 269 126 4072 Fragments arranged by size: 4072 712 320 269 246 154 135 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5063 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2387 2421 4225 4259 1903 Size: 34 50 34 366 34 1804 34 3678 Fragments arranged by size: 3678 1804 366 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2337 2694 4645 Size: 107 122 71 631 605 665 357 1951 Cuts at: 4645 136 Size: 1525 Fragments arranged by size: 1951 1525 665 631 605 357 122 107 71 BglII A'GATC_T Cuts at: 6029 6029 Size: 6034 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2389 2453 2484 2638 3039 3075 3227 4095 Size: 599 64 31 154 401 36 152 868 Cuts at: 4095 5251 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 868 599 401 154 152 64 36 31 BmgBI CAC'GTC Cuts at: 1318 3082 1318 Size: 1764 4270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3765 4723 442 Size: 416 53 83 399 24 2348 958 1753 Fragments arranged by size: 2348 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2737 3269 4694 1239 Size: 1498 532 1425 2579 Fragments arranged by size: 2579 1498 1425 532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 5024 5322 4159 Size: 865 298 4871 Fragments arranged by size: 4871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2040 2974 4697 882 Size: 617 541 934 1723 2219 Fragments arranged by size: 2219 1723 934 617 541 BsaAI yAC'GTr Cuts at: 493 1204 3238 3825 493 Size: 711 2034 587 2702 Fragments arranged by size: 2702 2034 711 587 BsaBI GATnn'nnATC Cuts at: 6028 6028 Size: 6034 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3841 4223 4858 275 Size: 53 83 186 936 2308 382 635 1451 Fragments arranged by size: 2308 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4467 4865 5139 5286 5697 4467 Size: 398 274 147 411 4804 Fragments arranged by size: 4804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2585 2615 2870 2900 1178 Size: 30 165 30 1182 30 255 30 4312 Fragments arranged by size: 4312 1182 255 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 1186 Size: 887 5147 BseYI C'CCAG_C Cuts at: 2411 2584 5237 5733 2411 Size: 173 2653 496 2712 Fragments arranged by size: 2712 2653 496 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 4816 Fragments arranged by size: 4816 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3215 3484 4245 5273 1540 Size: 275 186 1214 269 761 1028 2301 Fragments arranged by size: 2301 1214 1028 761 275 269 186 BsiHKAI G_wGCw'C Cuts at: 2453 2888 3039 4095 4180 5251 2453 Size: 435 151 1056 85 1071 3236 Fragments arranged by size: 3236 1071 1056 435 151 85 BsmI GAATG_Cn' Cuts at: 2123 3545 3638 2123 Size: 1422 93 4519 Fragments arranged by size: 4519 1422 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2349 2389 2453 2484 2638 2888 Size: 399 313 246 40 64 31 154 250 Cuts at: 2888 2958 3039 3075 3227 3353 4095 4180 5251 Size: 70 81 36 152 126 742 85 1071 Cuts at: 5251 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 313 250 246 154 152 126 85 81 70 64 40 36 31 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3273 3923 5653 3273 Size: 650 1730 3654 Fragments arranged by size: 3654 1730 650 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2462 2550 3389 844 Size: 1618 88 839 3489 Fragments arranged by size: 3489 1618 839 88 BsrBI CCG'CTC Cuts at: 1394 1823 2253 2312 2601 3064 3921 1394 Size: 429 430 59 289 463 857 3507 Fragments arranged by size: 3507 857 463 430 429 289 59 BsrDI GCAATG_nn' Cuts at: 65 1248 4529 4703 65 Size: 1183 3281 174 1396 Fragments arranged by size: 3281 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3214 4678 4865 1700 Size: 110 1404 1464 187 2869 Fragments arranged by size: 2869 1464 1404 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6034 BssSI C'ACGA_G Cuts at: 4094 5106 4094 Size: 1012 5022 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 2465 2819 3256 1184 Size: 1281 354 437 3962 Fragments arranged by size: 3962 1281 437 354 BstYI r'GATC_y Cuts at: 1972 3090 3135 3364 3731 4116 4133 5585 5671 Size: 1118 45 229 367 385 17 1452 86 Cuts at: 5671 5683 6029 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1118 385 367 346 229 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2085 2190 2085 Size: 105 5929 BtgI C'CryG_G Cuts at: 513 1065 2914 513 Size: 552 1849 3633 Fragments arranged by size: 3633 1849 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 2155 2734 2806 3550 4344 4364 2155 Size: 579 72 744 794 20 3825 Fragments arranged by size: 3825 794 744 579 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2832 3212 3481 Size: 54 1425 50 275 18 1002 380 269 Cuts at: 3481 4370 5999 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1002 889 380 275 269 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3212 3481 1537 Size: 275 1400 269 4090 Fragments arranged by size: 4090 1400 275 269 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 3964 1353 Size: 738 1873 3423 Fragments arranged by size: 3423 1873 738 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4621 4995 5141 387 Size: 1064 138 3032 374 146 1280 Fragments arranged by size: 3032 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2737 3269 4097 4694 5481 5749 Size: 393 483 622 532 828 597 787 268 Cuts at: 5749 1239 Size: 1524 Fragments arranged by size: 1524 828 787 622 597 532 483 393 268 EcoO109I rG'GnC_Cy Cuts at: 2140 2288 2392 2588 2634 2635 2670 3224 3321 Size: 148 104 196 46 1 35 554 97 Cuts at: 3321 2140 Size: 4853 Fragments arranged by size: 4853 554 196 148 104 97 46 35 1 EcoRI G'AATT_C Cuts at: 3265 3343 3721 3265 Size: 78 378 5578 Fragments arranged by size: 5578 378 78 FspI TGC'GCA Cuts at: 2877 4540 2877 Size: 1663 4371 FspAI rTGC'GCAy Cuts at: 2877 2877 Size: 6034 HaeII r_GCGC'y Cuts at: 1678 2054 2653 3195 5181 1678 Size: 376 599 542 1986 2531 Fragments arranged by size: 2531 1986 599 542 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2705 2737 2852 2884 4675 Size: 32 135 32 1133 32 115 32 1791 Cuts at: 4675 4707 4749 4781 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1791 1133 135 115 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2170 2782 2908 3378 3624 669 Size: 988 281 232 612 126 470 246 3079 Fragments arranged by size: 3079 988 612 470 281 246 232 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KpnI G_GTAC'C Cuts at: 3359 3359 Size: 6034 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 5148 5332 1305 Size: 1719 2124 184 2007 Fragments arranged by size: 2124 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2834 10 Size: 54 1425 343 1002 3210 Fragments arranged by size: 3210 1425 1002 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2712 3992 4351 4510 518 Size: 648 1546 1280 359 159 2042 Fragments arranged by size: 2042 1546 1280 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2258 2364 2532 2559 2584 2775 2827 2928 Size: 652 106 168 27 25 191 52 101 Cuts at: 2928 2946 2982 3077 3450 4127 5275 5520 1606 Size: 18 36 95 373 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 373 245 191 168 106 101 95 52 36 27 25 18 NcoI C'CATG_G Cuts at: 513 1065 2914 513 Size: 552 1849 3633 Fragments arranged by size: 3633 1849 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 3392 4937 1132 Size: 97 2163 1545 2229 Fragments arranged by size: 2229 2163 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6034 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 2830 1108 Size: 1722 4312 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4103 4135 5616 5648 4103 Size: 32 1481 32 4489 Fragments arranged by size: 4489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2288 2670 2140 Size: 148 382 5504 Fragments arranged by size: 5504 382 148 PshAI GACnn'nnGTC Cuts at: 1613 3104 1613 Size: 1491 4543 PsiI TTA'TAA Cuts at: 3604 3604 Size: 6034 PspOMI G'GGCC_C Cuts at: 2480 2634 3223 2480 Size: 154 589 5291 Fragments arranged by size: 5291 589 154 PstI C_TGCA'G Cuts at: 830 2729 3386 830 Size: 1899 657 3478 Fragments arranged by size: 3478 1899 657 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2364 2584 2827 2364 Size: 220 243 5571 Fragments arranged by size: 5571 243 220 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SanDI GG'GwC_CC Cuts at: 2140 2288 2140 Size: 148 5886 SapI GCTCTTCn'nnn_ Cuts at: 2091 2091 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3386 3386 Size: 6034 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SfcI C'TryA_G Cuts at: 826 1045 2725 3382 4517 5198 5389 826 Size: 219 1680 657 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 657 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 2032 3230 3361 1872 Size: 160 1198 131 4545 Fragments arranged by size: 4545 1198 160 131 SmlI C'TyrA_G Cuts at: 820 1017 1943 4138 5039 5301 820 Size: 197 926 2195 901 262 1553 Fragments arranged by size: 2195 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 3392 1132 Size: 2260 3774 SspI AAT'ATT Cuts at: 5 52 3958 5 Size: 47 3906 2081 Fragments arranged by size: 3906 2081 47 StuI AGG'CCT Cuts at: 1517 2195 1517 Size: 678 5356 StyI C'CwwG_G Cuts at: 513 1065 2914 3473 513 Size: 552 1849 559 3074 Fragments arranged by size: 3074 1849 559 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4061 4078 4231 4416 4861 325 Size: 1255 2481 17 153 185 445 1498 Fragments arranged by size: 2481 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3295 4280 5992 Size: 274 80 33 51 1443 1318 985 1712 Cuts at: 5992 96 Size: 138 Fragments arranged by size: 1712 1443 1318 985 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2198 2230 2870 2902 5994 6026 2198 Size: 32 640 32 3092 32 2206 Fragments arranged by size: 3092 2206 640 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6034 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2432 2947 2432 Size: 515 5519 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 2030 3228 3359 1870 Size: 160 1198 131 4545 Fragments arranged by size: 4545 1198 160 131 XmnI GAAnn'nnTTC Cuts at: 4163 4163 Size: 6034 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI