Restriction map for
pFN21AB6642
(Linear) (Six-base) MAPSORT of: KIBB6642.seq Check: 2822 from: 1 to: 1329
KIBB6642.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 457 1329
Size: 457 872
AccI GT'mk_AC
Cuts at: 0 1103 1329
Size: 1103 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1329
Size: 110 1219
AfeI AGC'GCT
Cuts at: 0 646 1329
Size: 646 683
AflIII A'CryG_T
Cuts at: 0 1076 1329
Size: 1076 253
AlwNI CAG_nnn'CTG
Cuts at: 0 390 825 1329
Size: 390 435 504
Fragments arranged by size:
504 435 390
ApaI G_GGCC'C
Cuts at: 0 479 633 1222 1329
Size: 479 154 589 107
Fragments arranged by size:
589 479 154 107
ApaLI G'TGCA_C
Cuts at: 0 444 1030 1329
Size: 444 586 299
Fragments arranged by size:
586 444 299
ApoI r'AATT_y
Cuts at: 0 1260 1329
Size: 1260 69
AvaI C'yCGr_G
Cuts at: 0 25 478 1223 1329
Size: 25 453 745 106
Fragments arranged by size:
745 453 106 25
BanI G'GyrC_C
Cuts at: 0 1111 1329
Size: 1111 218
BanII G_rGCy'C
Cuts at: 0 98 344 479 633 953 1222 1329
Size: 98 246 135 154 320 269 107
Fragments arranged by size:
320 269 246 154 135 107 98
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 416 1329
Size: 382 34 913
Fragments arranged by size:
913 382 34
BglI GCCn_nnn'nGGC
Cuts at: 0 332 689 1329
Size: 332 357 640
Fragments arranged by size:
640 357 332
Bme1580I G_kGCm'C
Cuts at: 0 384 448 479 633 1034 1070 1222 1329
Size: 384 64 31 154 401 36 152 107
Fragments arranged by size:
401 384 154 152 107 64 36 31
BmgBI CAC'GTC
Cuts at: 0 1077 1329
Size: 1077 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1264 1329
Size: 732 532 65
Fragments arranged by size:
732 532 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 35 969 1329
Size: 35 934 360
Fragments arranged by size:
934 360 35
BsaAI yAC'GTr
Cuts at: 0 1233 1329
Size: 1233 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 580 610 865 895 1329
Size: 580 30 255 30 434
Fragments arranged by size:
580 434 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 68 1329
Size: 68 1261
BseYI C'CCAG_C
Cuts at: 0 406 579 1329
Size: 406 173 750
Fragments arranged by size:
750 406 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1329
Size: 376 953
BsiEI CG_ry'CG
Cuts at: 0 1210 1329
Size: 1210 119
BsiHKAI G_wGCw'C
Cuts at: 0 448 883 1034 1329
Size: 448 435 151 295
Fragments arranged by size:
448 435 295 151
BsmI GAATG_Cn'
Cuts at: 0 118 1329
Size: 118 1211
Bsp1286I G_dGCh'C
Cuts at: 0 98 344 384 448 479 633 883 953
Size: 98 246 40 64 31 154 250 70
Cuts at: 953 1034 1070 1222 1329
Size: 81 36 152 107
Fragments arranged by size:
250 246 154 152 107 98 81 70
64 40 36 31
BspHI T'CATG_A
Cuts at: 0 1268 1329
Size: 1268 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 457 545 1329
Size: 457 88 784
Fragments arranged by size:
784 457 88
BsrBI CCG'CTC
Cuts at: 0 248 307 596 1059 1329
Size: 248 59 289 463 270
Fragments arranged by size:
463 289 270 248 59
BsrFI r'CCGG_y
Cuts at: 0 1209 1329
Size: 1209 120
BstEII G'GTnAC_C
Cuts at: 0 460 814 1251 1329
Size: 460 354 437 78
Fragments arranged by size:
460 437 354 78
BstYI r'GATC_y
Cuts at: 0 1085 1130 1329
Size: 1085 45 199
Fragments arranged by size:
1085 199 45
Bsu36I CC'TnA_GG
Cuts at: 0 80 185 1329
Size: 80 105 1144
Fragments arranged by size:
1144 105 80
BtgI C'CryG_G
Cuts at: 0 909 1329
Size: 909 420
BtsI GCAGTG_nn'
Cuts at: 0 150 729 801 1329
Size: 150 579 72 528
Fragments arranged by size:
579 528 150 72
EaeI y'GGCC_r
Cuts at: 0 827 1207 1329
Size: 827 380 122
Fragments arranged by size:
827 380 122
EagI C'GGCC_G
Cuts at: 0 1207 1329
Size: 1207 122
EarI CTCTTCn'nnn_
Cuts at: 0 86 1329
Size: 86 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 732 1264 1329
Size: 110 622 532 65
Fragments arranged by size:
622 532 110 65
EcoO109I rG'GnC_Cy
Cuts at: 0 135 283 387 583 629 630 665 1219
Size: 135 148 104 196 46 1 35 554
Cuts at: 1219 1316 1329
Size: 97 13
Fragments arranged by size:
554 196 148 135 104 97 46 35
13 1
EcoRI G'AATT_C
Cuts at: 0 1260 1329
Size: 1260 69
FspI TGC'GCA
Cuts at: 0 872 1329
Size: 872 457
FspAI rTGC'GCAy
Cuts at: 0 872 1329
Size: 872 457
HaeII r_GCGC'y
Cuts at: 0 49 648 1190 1329
Size: 49 599 542 139
Fragments arranged by size:
599 542 139 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 847 879 1329
Size: 700 32 115 32 450
Fragments arranged by size:
700 450 115 32 32
HincII GTy'rAC
Cuts at: 0 165 777 903 1329
Size: 165 612 126 426
Fragments arranged by size:
612 426 165 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1329
Size: 1019 310
MscI TGG'CCA
Cuts at: 0 829 1329
Size: 829 500
MslI CAynn'nnrTG
Cuts at: 0 707 1329
Size: 707 622
MspA1I CmG'CkG
Cuts at: 0 253 359 527 554 579 770 822 923
Size: 253 106 168 27 25 191 52 101
Cuts at: 923 941 977 1072 1329
Size: 18 36 95 257
Fragments arranged by size:
257 253 191 168 106 101 95 52
36 27 25 18
NcoI C'CATG_G
Cuts at: 0 909 1329
Size: 909 420
PflMI CCAn_nnn'nTGG
Cuts at: 0 825 1329
Size: 825 504
PpuMI rG'GwC_Cy
Cuts at: 0 135 283 665 1329
Size: 135 148 382 664
Fragments arranged by size:
664 382 148 135
PshAI GACnn'nnGTC
Cuts at: 0 1099 1329
Size: 1099 230
PspOMI G'GGCC_C
Cuts at: 0 475 629 1218 1329
Size: 475 154 589 111
Fragments arranged by size:
589 475 154 111
PstI C_TGCA'G
Cuts at: 0 724 1329
Size: 724 605
PvuII CAG'CTG
Cuts at: 0 359 579 822 1329
Size: 359 220 243 507
Fragments arranged by size:
507 359 243 220
SanDI GG'GwC_CC
Cuts at: 0 135 283 1329
Size: 135 148 1046
Fragments arranged by size:
1046 148 135
SapI GCTCTTCn'nnn_
Cuts at: 0 86 1329
Size: 86 1243
SfcI C'TryA_G
Cuts at: 0 720 1329
Size: 720 609
SmaI CCC'GGG
Cuts at: 0 27 1225 1329
Size: 27 1198 104
Fragments arranged by size:
1198 104 27
StuI AGG'CCT
Cuts at: 0 190 1329
Size: 190 1139
StyI C'CwwG_G
Cuts at: 0 909 1329
Size: 909 420
TatI w'GTAC_w
Cuts at: 0 1290 1329
Size: 1290 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 865 897 1329
Size: 193 32 640 32 432
Fragments arranged by size:
640 432 193 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 942 1329
Size: 427 515 387
Fragments arranged by size:
515 427 387
XmaI C'CCGG_G
Cuts at: 0 25 1223 1329
Size: 25 1198 106
Fragments arranged by size:
1198 106 25
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BcgI BglI Bme1580I BmgBI
BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BstEII
BstYI Bsu36I BtgI BtsI EaeI EagI EarI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI PflMI PpuMI PshAI PspOMI
PstI PvuII SanDI SapI SfcI SmaI StuI StyI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiWI
BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRV FalI FseI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI