################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BbrPI BciVI BfrI BglII BlnI BpmI BsaBI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflIII 1128 ACACGT A^C[GA][CT]GT AocI 616 CCTCAGG CC^T[ACGT]AGG Aor51HI 1248 AGCGCT AGC^GCT BamHI 383 GGATCC G^GATCC BbeI 1162 GGCGCC GGCGC^C BbsI 1357 GTCTTC GAAGAC(2/6) BbvCI 1102 GCTGAGG CCTCAGC(-5/-2) BcgI 108 CGAGGTAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 764 TGATCA T^GATCA BfaI 895 CTAG C^TAG BpuAI 1357 GTCTTC GAAGAC(2/6) BsaI 59 GAGACC GGTCTC(1/5) BsmAI 59 GAGAC GTCTC(1/5) Bsp1407I 1052 TGTACA T^GTACA BsrGI 1052 TGTACA T^GTACA BstXI 297 CCAGCATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 616 CCTCAGG CC^T[ACGT]AGG DrdI 1138 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 748 GAAGAG CTCTTC(1/4) Eco47III 1248 AGCGCT AGC^GCT Eco81I 616 CCTCAGG CC^T[ACGT]AGG FbaI 764 TGATCA T^GATCA HgaI 643 GACGC GACGC(5/10) KasI 1162 GGCGCC G^GCGCC Ksp632I 748 GAAGAG CTCTTC(1/4) MaeI 895 CTAG C^TAG NarI 1162 GGCGCC GG^CGCC PpuMI 1198 GGGACCC [GA]G^G[AT]CC[CT] SfiI 87 GGCCTCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1162 GGCGCC GGC^GCC SmaI 1232 CCCGGG CCC^GGG SspBI 1052 TGTACA T^GTACA Tsp509I 351 AATT ^AATT XmaCI 1232 CCCGGG C^CCGGG XmaI 1232 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 643 GACGCC G[GA]^CG[CT]C 1162 GGCGCC ApaI 1071 GGGCCC GGGCC^C 1350 GGGCCC AvaI 56 CCCGAG C^[CT]CG[GA]G 1232 CCCGGG BanII 1071 GGGCCC G[GA]GC[CT]^C 1350 GGGCCC BlpI 393 GCTGAGC GC^T[ACGT]AGC 820 GCTGAGC Bpu1102I 393 GCTGAGC GC^T[ACGT]AGC 820 GCTGAGC BsaAI 838 TACGTG [CT]AC^GT[GA] 1129 CACGTA BsaHI 643 GACGCC G[GA]^CG[CT]C 1162 GGCGCC BseRI 40 GAGGAG GAGGAG(10/8) 913 GAGGAG BsoBI 56 CCCGAG C^[CT]CG[GA]G 1232 CCCGGG BspMI 62 ACCTGC ACCTGC(4/8) 539 ACCTGC BsrDI 465 CATTGC GCAATG(2/0) 873 CATTGC BssSI 182 CACGAG CACGAG(-5/-1) 652 CTCGTG BstYI 383 GGATCC [GA]^GATC[CT] 974 GGATCT CelII 393 GCTGAGC GC^T[ACGT]AGC 820 GCTGAGC DraIII 562 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 1338 CACCCTGTG DsaI 101 CCACGG C^C[GA][CT]GG 1037 CCACGG EcoNI 63 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 954 CCTTGATGAGG EcoT14I 702 CCTTGG C^C[AT][AT]GG 776 CCTTGG Hin1I 643 GACGCC G[GA]^CG[CT]C 1162 GGCGCC HphI 1217 TCACC GGTGA(8/7) 1361 TCACC MflI 383 GGATCC [GA]^GATC[CT] 974 GGATCT MslI 439 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 952 CACCTTGATG PleI 478 GACTC GAGTC(4/5) 185 GAGTC StuI 620 AGGCCT AGG^CCT 1205 AGGCCT StyI 702 CCTTGG C^C[AT][AT]GG 776 CCTTGG TfiI 19 GAATC G^A[AT]TC 308 GAATC XcmI 443 CCAGTGCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1226 CCATGTCCCGGGTGG XhoII 383 GGATCC [GA]^GATC[CT] 974 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI