(Circular) (Six-base) MAPSORT of: pFN21AE6436.seq Check: 4763 from: 1 to: 6085 pFN21AE6436 6085 bp With 182 enzymes: SgfI * May 20, 2014 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2553 2553 Size: 6085 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3428 1937 Size: 1491 4594 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2038 2093 2195 2258 4148 5532 5800 1632 Size: 406 55 102 63 1890 1384 268 1917 Fragments arranged by size: 1917 1890 1384 406 268 102 63 55 AfeI AGC'GCT Cuts at: 3255 3255 Size: 6085 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 3133 4984 5961 1203 Size: 1930 1851 977 1327 Fragments arranged by size: 1930 1851 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 2448 1166 Size: 1282 4803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2561 1184 Size: 1377 4708 AlwNI CAG_nnn'CTG Cuts at: 2398 2610 3055 3828 5400 2398 Size: 212 445 773 1572 3083 Fragments arranged by size: 3083 1572 773 445 212 ApaI G_GGCC'C Cuts at: 3080 3359 3080 Size: 279 5806 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 3394 3620 3772 5881 1493 Size: 1901 226 152 2109 1697 Fragments arranged by size: 2109 1901 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2061 3237 3410 1870 Size: 73 118 1176 173 4545 Fragments arranged by size: 4545 1176 173 118 73 BamHI G'GATC_C Cuts at: 2388 3415 3782 2388 Size: 1027 367 4691 Fragments arranged by size: 4691 1027 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2672 3167 3406 4861 Size: 325 217 627 357 528 495 239 1455 Cuts at: 4861 618 Size: 1842 Fragments arranged by size: 1842 1455 627 528 495 357 325 239 217 BanII G_rGCy'C Cuts at: 1391 3080 3359 3404 1391 Size: 1689 279 45 4072 Fragments arranged by size: 4072 1689 279 45 BbeI G_GCGC'C Cuts at: 3171 3171 Size: 6085 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3355 928 Size: 971 1456 3658 Fragments arranged by size: 3658 1456 971 BbvCI CC'TCA_GC Cuts at: 3108 3108 Size: 6085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2102 2136 4276 4310 1903 Size: 34 165 34 2140 34 3678 Fragments arranged by size: 3678 2140 165 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 2769 1548 Size: 1221 4864 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 2282 3075 Size: 107 122 71 631 605 427 183 793 Cuts at: 3075 4696 136 Size: 1621 1525 Fragments arranged by size: 1621 1525 793 631 605 427 183 122 107 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 2399 2826 3502 2399 Size: 427 676 4982 Fragments arranged by size: 4982 676 427 Bme1580I G_kGCm'C Cuts at: 1790 2675 3080 3359 4146 5302 1790 Size: 885 405 279 787 1156 2573 Fragments arranged by size: 2573 1156 885 787 405 279 BmgBI CAC'GTC Cuts at: 1318 3295 1318 Size: 1977 4108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2442 3816 4774 Size: 416 53 83 399 24 1025 1374 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1753 1374 1025 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4745 1239 Size: 3506 2579 Bpu10I CC'TnA_GC Cuts at: 2495 2504 3108 2495 Size: 9 604 5472 Fragments arranged by size: 5472 604 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4210 5075 5373 4210 Size: 865 298 4922 Fragments arranged by size: 4922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2058 4748 882 Size: 617 559 2690 2219 Fragments arranged by size: 2690 2219 617 559 BsaAI yAC'GTr Cuts at: 493 1204 2845 3136 3876 493 Size: 711 1641 291 740 2702 Fragments arranged by size: 2702 1641 740 711 291 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2649 3168 3892 4274 Size: 53 83 186 936 1116 519 724 382 Cuts at: 4274 4909 275 Size: 635 1451 Fragments arranged by size: 1451 1116 936 724 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2474 2504 1178 Size: 30 165 30 1071 30 4759 Fragments arranged by size: 4759 1071 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2933 1186 Size: 874 873 4338 Fragments arranged by size: 4338 874 873 BseYI C'CCAG_C Cuts at: 2250 2254 2260 2680 2830 3327 3370 5288 5784 Size: 4 6 420 150 497 43 1918 496 Cuts at: 5784 2250 Size: 2551 Fragments arranged by size: 2551 1918 497 496 420 150 43 6 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5512 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3535 4296 5324 1540 Size: 275 186 1534 761 1028 2301 Fragments arranged by size: 2301 1534 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4146 4231 5302 4146 Size: 85 1071 4929 Fragments arranged by size: 4929 1071 85 BsmI GAATG_Cn' Cuts at: 3596 3689 3596 Size: 93 5992 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2675 3080 3359 3404 4146 4231 5302 Size: 399 885 405 279 45 742 85 1071 Cuts at: 5302 1391 Size: 2174 Fragments arranged by size: 2174 1071 885 742 405 399 279 85 45 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3974 5704 3974 Size: 1730 4355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2553 3440 844 Size: 1232 477 887 3489 Fragments arranged by size: 3489 1232 887 477 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 2877 4580 4754 65 Size: 1183 1221 408 1703 174 1396 Fragments arranged by size: 1703 1396 1221 1183 408 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4729 4916 1700 Size: 110 2919 187 2869 Fragments arranged by size: 2919 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3057 96 Size: 2961 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 2187 2657 4145 5157 2187 Size: 470 1488 1012 3115 Fragments arranged by size: 3115 1488 1012 470 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6085 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2309 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2388 2979 3415 3782 4167 4184 5636 5722 Size: 416 591 436 367 385 17 1452 86 Cuts at: 5722 5734 6080 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 591 436 416 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2622 2622 Size: 6085 BtgI C'CryG_G Cuts at: 513 1065 2004 2106 3042 513 Size: 552 939 102 936 3556 Fragments arranged by size: 3556 939 936 552 102 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2626 487 Size: 683 56 1400 3946 Fragments arranged by size: 3946 1400 683 56 BtsI GCAGTG_nn' Cuts at: 2611 3601 4395 4415 2611 Size: 990 794 20 4281 Fragments arranged by size: 4281 990 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6085 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DraIII CAC_nnn'GTG Cuts at: 2572 3348 2572 Size: 776 5309 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3149 5092 809 Size: 507 1833 1943 1802 Fragments arranged by size: 1943 1833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050 Size: 54 1425 50 275 18 1702 889 1629 Cuts at: 6050 8 Size: 43 Fragments arranged by size: 1702 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3532 1537 Size: 275 1720 4090 Fragments arranged by size: 4090 1720 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2748 4015 1353 Size: 1395 1267 3423 Fragments arranged by size: 3423 1395 1267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4672 5046 5192 387 Size: 1064 138 3083 374 146 1280 Fragments arranged by size: 3083 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2038 2093 2195 2258 4148 4745 5532 Size: 393 406 55 102 63 1890 597 787 Cuts at: 5532 5800 1239 Size: 268 1524 Fragments arranged by size: 1890 1524 787 597 406 393 268 102 63 55 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2963 2072 Size: 891 5194 EcoO109I rG'GnC_Cy Cuts at: 2091 3076 3112 3204 3356 2091 Size: 985 36 92 152 4820 Fragments arranged by size: 4820 985 152 92 36 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 2839 3171 3257 5232 1678 Size: 1161 332 86 1975 2531 Fragments arranged by size: 2531 1975 1161 332 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 4726 4758 4800 Size: 32 135 32 902 32 2220 32 42 Cuts at: 4800 4832 1373 Size: 32 2626 Fragments arranged by size: 2626 2220 902 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KasI G'GCGC_C Cuts at: 3167 3167 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5199 5383 1305 Size: 3894 184 2007 Fragments arranged by size: 3894 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4263 Fragments arranged by size: 4263 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2448 2961 4043 4402 4561 518 Size: 648 1282 513 1082 359 159 2042 Fragments arranged by size: 2042 1282 1082 648 513 359 159 MspA1I CmG'CkG Cuts at: 1606 2254 2395 3268 3501 4178 5326 5571 1606 Size: 648 141 873 233 677 1148 245 2120 Fragments arranged by size: 2120 1148 873 677 648 245 233 141 NarI GG'CG_CC Cuts at: 3168 3168 Size: 6085 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 3443 4988 1132 Size: 97 2214 1545 2229 Fragments arranged by size: 2229 2214 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3204 3204 Size: 6085 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 3076 3355 3076 Size: 279 5806 PstI C_TGCA'G Cuts at: 830 2540 3030 3280 3437 830 Size: 1710 490 250 157 3478 Fragments arranged by size: 3478 1710 490 250 157 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2254 2395 3268 2254 Size: 141 873 5071 Fragments arranged by size: 5071 873 141 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SanDI GG'GwC_CC Cuts at: 3204 3204 Size: 6085 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 4333 6045 4333 Size: 1712 4373 SfcI C'TryA_G Cuts at: 826 1045 2536 3026 3276 3433 4568 5249 5440 Size: 219 1491 490 250 157 1135 681 191 Cuts at: 5440 826 Size: 1471 Fragments arranged by size: 1491 1471 1135 681 490 250 219 191 157 SfiI GGCCn_nnn'nGGCC Cuts at: 2099 2099 Size: 6085 SfoI GGC'GCC Cuts at: 3169 3169 Size: 6085 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 3239 3412 1872 Size: 1367 173 4545 Fragments arranged by size: 4545 1367 173 SmlI C'TyrA_G Cuts at: 820 1017 1943 4189 5090 5352 820 Size: 197 926 2246 901 262 1553 Fragments arranged by size: 2246 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 2627 3212 1517 Size: 1110 585 4390 Fragments arranged by size: 4390 1110 585 StyI C'CwwG_G Cuts at: 513 1065 2004 2707 2781 3524 513 Size: 552 939 703 74 743 3074 Fragments arranged by size: 3074 939 743 703 552 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2132 2573 4112 4129 4282 4467 4912 Size: 1255 552 441 1539 17 153 185 445 Cuts at: 4912 325 Size: 1498 Fragments arranged by size: 1539 1498 1255 552 445 441 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2790 3057 4331 Size: 274 80 33 51 1443 813 267 1274 Cuts at: 4331 6043 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1274 813 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3290 3322 6045 6077 3290 Size: 32 2723 32 3298 Fragments arranged by size: 3298 2723 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6085 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2455 3238 2455 Size: 783 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 3237 3410 1870 Size: 1367 173 4545 Fragments arranged by size: 4545 1367 173 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SrfI SwaI