Restriction map for
pFN21AE6436
(Linear) (Six-base) MAPSORT of: KIEE6436.seq Check: 7378 from: 1 to: 1380
KIEE6436.orf
With 182 enzymes: SgfI *
May 20, 2014 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 548 1380
Size: 548 832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 88 190 253 1380
Size: 33 55 102 63 1127
Fragments arranged by size:
1127 102 63 55 33
AfeI AGC'GCT
Cuts at: 0 1250 1380
Size: 1250 130
AflIII A'CryG_T
Cuts at: 0 1128 1380
Size: 1128 252
AleI CACnn'nnGTG
Cuts at: 0 443 1380
Size: 443 937
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 1380
Size: 556 824
AlwNI CAG_nnn'CTG
Cuts at: 0 393 605 1050 1380
Size: 393 212 445 330
Fragments arranged by size:
445 393 330 212
ApaI G_GGCC'C
Cuts at: 0 1075 1354 1380
Size: 1075 279 26
Fragments arranged by size:
1075 279 26
AvaI C'yCGr_G
Cuts at: 0 56 1232 1380
Size: 56 1176 148
Fragments arranged by size:
1176 148 56
BamHI G'GATC_C
Cuts at: 0 383 1380
Size: 383 997
BanI G'GyrC_C
Cuts at: 0 139 667 1162 1380
Size: 139 528 495 218
Fragments arranged by size:
528 495 218 139
BanII G_rGCy'C
Cuts at: 0 1075 1354 1380
Size: 1075 279 26
Fragments arranged by size:
1075 279 26
BbeI G_GCGC'C
Cuts at: 0 1166 1380
Size: 1166 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 1350 1380
Size: 1350 30
BbvCI CC'TCA_GC
Cuts at: 0 1103 1380
Size: 1103 277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 97 131 1380
Size: 97 34 1249
Fragments arranged by size:
1249 97 34
BclI T'GATC_A
Cuts at: 0 764 1380
Size: 764 616
BglI GCCn_nnn'nGGC
Cuts at: 0 94 277 1070 1380
Size: 94 183 793 310
Fragments arranged by size:
793 310 183 94
BlpI GC'TnA_GC
Cuts at: 0 394 821 1380
Size: 394 427 559
Fragments arranged by size:
559 427 394
Bme1580I G_kGCm'C
Cuts at: 0 670 1075 1354 1380
Size: 670 405 279 26
Fragments arranged by size:
670 405 279 26
BmgBI CAC'GTC
Cuts at: 0 1290 1380
Size: 1290 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 1380
Size: 437 943
Bpu10I CC'TnA_GC
Cuts at: 0 490 499 1103 1380
Size: 490 9 604 277
Fragments arranged by size:
604 490 277 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 53 1380
Size: 53 1327
BsaAI yAC'GTr
Cuts at: 0 840 1131 1380
Size: 840 291 249
Fragments arranged by size:
840 291 249
BsaHI Gr'CG_yC
Cuts at: 0 644 1163 1380
Size: 644 519 217
Fragments arranged by size:
644 519 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 1380
Size: 469 30 881
Fragments arranged by size:
881 469 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 928 1380
Size: 55 873 452
Fragments arranged by size:
873 452 55
BseYI C'CCAG_C
Cuts at: 0 245 249 255 675 825 1322 1365 1380
Size: 245 4 6 420 150 497 43 15
Fragments arranged by size:
497 420 245 150 43 15 6 4
Bsp1286I G_dGCh'C
Cuts at: 0 670 1075 1354 1380
Size: 670 405 279 26
Fragments arranged by size:
670 405 279 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 548 1380
Size: 71 477 832
Fragments arranged by size:
832 477 71
BsrDI GCAATG_nn'
Cuts at: 0 464 872 1380
Size: 464 408 508
Fragments arranged by size:
508 464 408
BsrGI T'GTAC_A
Cuts at: 0 1052 1380
Size: 1052 328
BssSI C'ACGA_G
Cuts at: 0 182 652 1380
Size: 182 470 728
Fragments arranged by size:
728 470 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 1380
Size: 304 1076
BstYI r'GATC_y
Cuts at: 0 383 974 1380
Size: 383 591 406
Fragments arranged by size:
591 406 383
Bsu36I CC'TnA_GG
Cuts at: 0 617 1380
Size: 617 763
BtgI C'CryG_G
Cuts at: 0 101 1037 1380
Size: 101 936 343
Fragments arranged by size:
936 343 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 621 1380
Size: 621 759
BtsI GCAGTG_nn'
Cuts at: 0 606 1380
Size: 606 774
DraIII CAC_nnn'GTG
Cuts at: 0 567 1343 1380
Size: 567 776 37
Fragments arranged by size:
776 567 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 1144 1380
Size: 1144 236
EarI CTCTTCn'nnn_
Cuts at: 0 743 1380
Size: 743 637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 88 190 253 1380
Size: 33 55 102 63 1127
Fragments arranged by size:
1127 102 63 55 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 958 1380
Size: 67 891 422
Fragments arranged by size:
891 422 67
EcoO109I rG'GnC_Cy
Cuts at: 0 86 1071 1107 1199 1351 1380
Size: 86 985 36 92 152 29
Fragments arranged by size:
985 152 92 86 36 29
HaeII r_GCGC'y
Cuts at: 0 834 1166 1252 1380
Size: 834 332 86 128
Fragments arranged by size:
834 332 128 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1380
Size: 469 32 879
Fragments arranged by size:
879 469 32
KasI G'GCGC_C
Cuts at: 0 1162 1380
Size: 1162 218
MslI CAynn'nnrTG
Cuts at: 0 443 956 1380
Size: 443 513 424
Fragments arranged by size:
513 443 424
MspA1I CmG'CkG
Cuts at: 0 249 390 1263 1380
Size: 249 141 873 117
Fragments arranged by size:
873 249 141 117
NarI GG'CG_CC
Cuts at: 0 1163 1380
Size: 1163 217
PpuMI rG'GwC_Cy
Cuts at: 0 1199 1380
Size: 1199 181
PspOMI G'GGCC_C
Cuts at: 0 1071 1350 1380
Size: 1071 279 30
Fragments arranged by size:
1071 279 30
PstI C_TGCA'G
Cuts at: 0 535 1025 1275 1380
Size: 535 490 250 105
Fragments arranged by size:
535 490 250 105
PvuII CAG'CTG
Cuts at: 0 249 390 1263 1380
Size: 249 141 873 117
Fragments arranged by size:
873 249 141 117
SanDI GG'GwC_CC
Cuts at: 0 1199 1380
Size: 1199 181
SfcI C'TryA_G
Cuts at: 0 531 1021 1271 1380
Size: 531 490 250 109
Fragments arranged by size:
531 490 250 109
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 94 1380
Size: 94 1286
SfoI GGC'GCC
Cuts at: 0 1164 1380
Size: 1164 216
SmaI CCC'GGG
Cuts at: 0 1234 1380
Size: 1234 146
StuI AGG'CCT
Cuts at: 0 622 1207 1380
Size: 622 585 173
Fragments arranged by size:
622 585 173
StyI C'CwwG_G
Cuts at: 0 702 776 1380
Size: 702 74 604
Fragments arranged by size:
702 604 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 127 568 1380
Size: 127 441 812
Fragments arranged by size:
812 441 127
TatI w'GTAC_w
Cuts at: 0 785 1052 1380
Size: 785 267 328
Fragments arranged by size:
785 328 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1285 1317 1380
Size: 1285 32 63
Fragments arranged by size:
1285 63 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 1233 1380
Size: 450 783 147
Fragments arranged by size:
783 450 147
XmaI C'CCGG_G
Cuts at: 0 1232 1380
Size: 1232 148
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI Bpu10I BsaI
BsaAI BsaHI BsaXI BseRI BseYI Bsp1286I BspMI BsrDI
BsrGI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MslI MspA1I NarI PpuMI PspOMI PstI PvuII
SanDI SfcI SfiI SfoI SmaI StuI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BglII BmtI BplI BpmI BpuEI BsaBI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI EaeI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI