Restriction map for
pFN21AE4527
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 88 GTCGAC GT^[AC][GT]AC
AlwI 438 GGATC GGATC(4/5)
Aor51HI 140 AGCGCT AGC^GCT
AseI 304 ATTAAT AT^TAAT
AsnI 304 ATTAAT AT^TAAT
AspHI 993 GAGCAC G[AT]GC[AT]^C
AvaII 778 GGTCC G^G[AT]CC
AvrII 168 CCTAGG C^CTAGG
BanII 190 GGGCTC G[GA]GC[CT]^C
BcnI 999 CCCGG CC^[GC]GG
BglII 117 AGATCT A^GATCT
BlnI 168 CCTAGG C^CTAGG
BpmI 181 CTGGAG CTGGAG(16/14)
BsaI 1516 GAGACC GGTCTC(1/5)
BsgI 959 GTGCAG GTGCAG(16/14)
BsiHKAI 993 GAGCAC G[AT]GC[AT]^C
BsmBI 13 GAGACG CGTCTC(1/5)
BstAPI 1084 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 160 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 140 AGCGCT AGC^GCT
EcoO109I 160 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 276 GAATTC G^AATTC
EcoT14I 168 CCTAGG C^C[AT][AT]GG
HaeII 140 AGCGCT [GA]GCGC^[CT]
HapII 1000 CCGG C^CGG
HincII 88 GTCGAC GT[CT]^[GA]AC
HindII 88 GTCGAC GT[CT]^[GA]AC
HindIII 1438 AAGCTT A^AGCTT
HpaII 1000 CCGG C^CGG
MaeII 1322 ACGT A^CGT
MspA1I 1403 CAGCTG C[AC]G^C[GT]G
MspI 1000 CCGG C^CGG
NciI 999 CCCGG CC^[GC]GG
NlaIII 1233 CATG CATG^
NspI 1232 GCATGC [GA]CATG^[CT]
PshAI 1378 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 304 ATTAAT AT^TAAT
PvuII 1403 CAGCTG CAG^CTG
SalI 88 GTCGAC G^TCGAC
SphI 1232 GCATGC GCATG^C
StyI 168 CCTAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 213 CGCG CG^CG
215 CGCG
AlwNI 1085 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1718 CAGAACCTG
BmyI 190 GGGCTC G[AGT]GC[ACT]^C
993 GAGCAC
Bsp1286I 190 GGGCTC G[AGT]GC[ACT]^C
993 GAGCAC
BssHII 212 GCGCGC G^CGCGC
214 GCGCGC
BstUI 213 CGCG CG^CG
215 CGCG
BstYI 117 AGATCT [GA]^GATC[CT]
438 GGATCT
MflI 117 AGATCT [GA]^GATC[CT]
438 GGATCT
MvnI 213 CGCG CG^CG
215 CGCG
PstI 869 CTGCAG CTGCA^G
1082 CTGCAG
SfaNI 101 GATGC GCATC(5/9)
1260 GCATC
SmlI 1412 CTCAAG C^T[CT][GA]AG
1560 CTTGAG
SspI 380 AATATT AAT^ATT
1497 AATATT
StuI 171 AGGCCT AGG^CCT
1032 AGGCCT
XhoII 117 AGATCT [GA]^GATC[CT]
438 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I