(Circular) (Six-base) MAPSORT of: pFN21AE4527.seq Check: 1205 from: 1 to: 6433 pFN21AE4527 6433 bp With 182 enzymes: SgfI * March 28, 2012 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3080 3080 Size: 6433 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4243 278 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2094 3776 1937 Size: 157 1682 4594 Fragments arranged by size: 4594 1682 157 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 4496 5880 6148 1632 Size: 508 2356 1384 268 1917 Fragments arranged by size: 2356 1917 1384 508 268 AfeI AGC'GCT Cuts at: 2147 2147 Size: 6433 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6236 AflIII A'CryG_T Cuts at: 1203 5332 6309 1203 Size: 4129 977 1327 Fragments arranged by size: 4129 1327 977 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 5162 5162 Size: 6433 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6433 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6433 AlwNI CAG_nnn'CTG Cuts at: 3095 3728 4176 5748 3095 Size: 633 448 1572 3780 Fragments arranged by size: 3780 1572 633 448 ApaLI G'TGCA_C Cuts at: 4490 5646 4490 Size: 1156 5277 ApoI r'AATT_y Cuts at: 1493 2281 2812 3337 3543 3581 3742 3968 4120 Size: 788 531 525 206 38 161 226 152 Cuts at: 4120 6229 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 788 531 525 226 206 161 152 38 AseI AT'TA_AT Cuts at: 160 1032 2310 4987 160 Size: 872 1278 2677 1606 Fragments arranged by size: 2677 1606 1278 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 3758 1870 Size: 73 1815 4545 Fragments arranged by size: 4545 1815 73 AvrII C'CTAG_G Cuts at: 2173 2173 Size: 6433 BamHI G'GATC_C Cuts at: 3763 4130 3763 Size: 367 6066 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3754 5209 618 Size: 325 217 627 1967 1455 1842 Fragments arranged by size: 1967 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2199 3752 1391 Size: 808 1553 4072 Fragments arranged by size: 4072 1553 808 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5462 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4624 4658 1903 Size: 34 2687 34 3678 Fragments arranged by size: 3678 2687 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4325 5535 4325 Size: 1210 5223 BclI T'GATC_A Cuts at: 1548 1548 Size: 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5044 136 Size: 107 122 71 631 605 3372 1525 Fragments arranged by size: 3372 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2122 6428 2122 Size: 4306 2127 BlpI GC'TnA_GC Cuts at: 3850 3850 Size: 6433 Bme1580I G_kGCm'C Cuts at: 1790 4494 5650 1790 Size: 2704 1156 2573 Fragments arranged by size: 2704 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3328 1318 Size: 2010 4423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2644 2730 3147 Size: 416 53 83 399 24 1227 86 417 Cuts at: 3147 4164 5122 442 Size: 1017 958 1753 Fragments arranged by size: 1753 1227 1017 958 417 416 399 86 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 5093 1239 Size: 968 2886 2579 Fragments arranged by size: 2886 2579 968 Bpu10I CC'TnA_GC Cuts at: 3225 3225 Size: 6433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3402 3586 4558 5423 5721 3402 Size: 184 972 865 298 4114 Fragments arranged by size: 4114 972 865 298 184 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3515 5096 882 Size: 617 2016 1581 2219 Fragments arranged by size: 2219 2016 1581 617 BsaAI yAC'GTr Cuts at: 493 1204 4224 493 Size: 711 3020 2702 Fragments arranged by size: 3020 2702 711 BsaBI GATnn'nnATC Cuts at: 6427 6427 Size: 6433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4240 4622 5257 275 Size: 53 83 186 936 2707 382 635 1451 Fragments arranged by size: 2707 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4866 5264 5538 5685 6096 4866 Size: 398 274 147 411 5203 Fragments arranged by size: 5203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3603 3633 1178 Size: 30 165 30 2200 30 3978 Fragments arranged by size: 3978 2200 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2283 2369 2734 1186 Size: 1097 86 365 4885 Fragments arranged by size: 4885 1097 365 86 BseYI C'CCAG_C Cuts at: 2786 2915 3525 5636 6132 2786 Size: 129 610 2111 496 3087 Fragments arranged by size: 3087 2111 610 496 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2985 1163 Size: 573 1249 4611 Fragments arranged by size: 4611 1249 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3883 4644 5672 1540 Size: 275 186 1882 761 1028 2301 Fragments arranged by size: 2301 1882 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3002 4494 4579 5650 3002 Size: 1492 85 1071 3785 Fragments arranged by size: 3785 1492 1071 85 BsmI GAATG_Cn' Cuts at: 3944 4037 3944 Size: 93 6340 BsmBI CGTCTCn'nnnn_ Cuts at: 2012 2012 Size: 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2199 3002 3752 4494 4579 5650 1391 Size: 399 409 803 750 742 85 1071 2174 Fragments arranged by size: 2174 1071 803 750 742 409 399 85 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 4322 6052 4322 Size: 1730 4703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3080 3568 3736 3788 844 Size: 2236 488 168 52 3489 Fragments arranged by size: 3489 2236 488 168 52 BsrBI CCG'CTC Cuts at: 1394 1823 4320 1394 Size: 429 2497 3507 Fragments arranged by size: 3507 2497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4928 5102 65 Size: 1183 3680 174 1396 Fragments arranged by size: 3680 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5077 5264 1700 Size: 110 3267 187 2869 Fragments arranged by size: 3267 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6433 BssHII G'CGCG_C Cuts at: 1925 2217 2219 1925 Size: 292 2 6139 Fragments arranged by size: 6139 292 2 BssSI C'ACGA_G Cuts at: 4493 5505 4493 Size: 1012 5421 BstAPI GCAn_nnn'nTGC Cuts at: 794 3095 794 Size: 2301 4132 BstBI TT'CG_AA Cuts at: 5237 5237 Size: 6433 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6433 BstYI r'GATC_y Cuts at: 1972 2122 2443 3763 4130 4515 4532 5984 6070 Size: 150 321 1320 367 385 17 1452 86 Cuts at: 6070 6082 6428 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 321 150 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4942 Fragments arranged by size: 4942 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5694 Fragments arranged by size: 5694 683 56 BtsI GCAGTG_nn' Cuts at: 2631 3949 4743 4763 2631 Size: 1318 794 20 4301 Fragments arranged by size: 4301 1318 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6398 DraI TTT'AAA Cuts at: 3737 3737 Size: 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5440 809 Size: 507 4124 1802 Fragments arranged by size: 4124 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3880 4769 6398 Size: 54 1425 50 275 18 2050 889 1629 Cuts at: 6398 8 Size: 43 Fragments arranged by size: 2050 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3880 1537 Size: 275 2068 4090 Fragments arranged by size: 4090 2068 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2256 2399 2532 3046 3175 3192 4363 1353 Size: 903 143 133 514 129 17 1171 3423 Fragments arranged by size: 3423 1171 903 514 143 133 129 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2129 5020 5394 5540 387 Size: 1064 138 540 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 540 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2207 4496 5093 5880 6148 1239 Size: 393 508 67 2289 597 787 268 1524 Fragments arranged by size: 2289 1524 787 597 508 393 268 67 EcoO109I rG'GnC_Cy Cuts at: 2166 2166 Size: 6433 EcoRI G'AATT_C Cuts at: 2281 3742 4120 2281 Size: 1461 378 4594 Fragments arranged by size: 4594 1461 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3221 3221 Size: 6433 FspI TGC'GCA Cuts at: 4939 4939 Size: 6433 HaeII r_GCGC'y Cuts at: 1678 2149 5580 1678 Size: 471 3431 2531 Fragments arranged by size: 3431 2531 471 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2741 2773 3443 Size: 32 135 32 525 32 612 32 670 Cuts at: 3443 3475 5074 5106 5148 5180 1373 Size: 32 1599 32 42 32 2626 Fragments arranged by size: 2626 1599 670 612 525 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2095 3777 4023 669 Size: 988 281 157 1682 246 3079 Fragments arranged by size: 3079 1682 988 281 246 157 HindIII A'AGCT_T Cuts at: 748 3443 748 Size: 2695 3738 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 4032 4032 Size: 6433 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3403 5547 5731 1305 Size: 2098 2144 184 2007 Fragments arranged by size: 2144 2098 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4611 Fragments arranged by size: 4611 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4391 4750 4909 518 Size: 648 3225 359 159 2042 Fragments arranged by size: 3225 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3410 3849 4526 5674 5919 1606 Size: 1804 439 677 1148 245 2120 Fragments arranged by size: 2120 1804 1148 677 439 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4942 Fragments arranged by size: 4942 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 3241 3791 5336 1132 Size: 97 2012 550 1545 2229 Fragments arranged by size: 2229 2012 1545 550 97 PasI CC'CwG_GG Cuts at: 1348 3068 1348 Size: 1720 4713 PciI A'CATG_T Cuts at: 5332 5332 Size: 6433 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6433 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2638 2670 2722 2754 4502 4534 6015 6047 2638 Size: 32 52 32 1748 32 1481 32 3024 Fragments arranged by size: 3024 1748 1481 52 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3387 1613 Size: 1774 4659 PsiI TTA'TAA Cuts at: 4003 4003 Size: 6433 PstI C_TGCA'G Cuts at: 830 2878 3091 3785 830 Size: 2048 213 694 3478 Fragments arranged by size: 3478 2048 694 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 3410 3410 Size: 6433 SalI G'TCGA_C Cuts at: 1936 2093 3775 1936 Size: 157 1682 4594 Fragments arranged by size: 4594 1682 157 SbfI CC_TGCA'GG Cuts at: 3785 3785 Size: 6433 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 2286 2874 3087 3385 3781 4916 5597 Size: 219 1241 588 213 298 396 1135 681 Cuts at: 5597 5788 826 Size: 191 1471 Fragments arranged by size: 1471 1241 1135 681 588 396 298 219 213 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 3760 1872 Size: 1888 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3417 3565 4537 5438 5700 820 Size: 197 926 1474 148 972 901 262 1553 Fragments arranged by size: 1553 1474 972 926 901 262 197 148 SnaBI TAC'GTA Cuts at: 493 4224 493 Size: 3731 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 3241 3791 1132 Size: 2109 550 3774 Fragments arranged by size: 3774 2109 550 SspI AAT'ATT Cuts at: 5 52 2387 3504 4357 5 Size: 47 2335 1117 853 2081 Fragments arranged by size: 2335 2081 1117 853 47 StuI AGG'CCT Cuts at: 1517 2178 3039 1517 Size: 661 861 4911 Fragments arranged by size: 4911 861 661 StyI C'CwwG_G Cuts at: 513 1065 2004 2173 3872 513 Size: 552 939 169 1699 3074 Fragments arranged by size: 3074 1699 939 552 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2513 3388 4460 4477 4630 4815 5260 Size: 1255 933 875 1072 17 153 185 445 Cuts at: 5260 325 Size: 1498 Fragments arranged by size: 1498 1255 1072 933 875 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2617 3719 4679 Size: 274 80 33 51 1443 640 1102 960 Cuts at: 4679 6391 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1102 960 640 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2692 2724 6393 6425 2692 Size: 32 3669 32 2700 Fragments arranged by size: 3669 2700 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6433 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 3758 1870 Size: 1888 4545 XmnI GAAnn'nnTTC Cuts at: 4562 4562 Size: 6433 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4241 276 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI