(Linear) (Six-base) MAPSORT of: KIEE4527.seq Check: 3282 from: 1 to: 1728 KIEE4527.orf With 182 enzymes: SgfI * March 22, 2012 18:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1075 1728 Size: 1075 653 AccI GT'mk_AC Cuts at: 0 89 1728 Size: 89 1639 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 1728 Size: 135 1593 AfeI AGC'GCT Cuts at: 0 142 1728 Size: 142 1586 AlwNI CAG_nnn'CTG Cuts at: 0 1090 1723 1728 Size: 1090 633 5 Fragments arranged by size: 1090 633 5 ApoI r'AATT_y Cuts at: 0 276 807 1332 1538 1576 1728 Size: 276 531 525 206 38 152 Fragments arranged by size: 531 525 276 206 152 38 AseI AT'TA_AT Cuts at: 0 305 1728 Size: 305 1423 AvrII C'CTAG_G Cuts at: 0 168 1728 Size: 168 1560 BanII G_rGCy'C Cuts at: 0 194 1728 Size: 194 1534 BglII A'GATC_T Cuts at: 0 117 1728 Size: 117 1611 BmgBI CAC'GTC Cuts at: 0 1323 1728 Size: 1323 405 BmrI ACTGGGnnnn_n' Cuts at: 0 639 725 1142 1728 Size: 639 86 417 586 Fragments arranged by size: 639 586 417 86 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1728 Size: 202 1526 Bpu10I CC'TnA_GC Cuts at: 0 1220 1728 Size: 1220 508 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1397 1581 1728 Size: 1397 184 147 Fragments arranged by size: 1397 184 147 BsaI GGTCTCn'nnnn_ Cuts at: 0 1510 1728 Size: 1510 218 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1598 1628 1728 Size: 1598 30 100 Fragments arranged by size: 1598 100 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 278 364 729 1728 Size: 278 86 365 999 Fragments arranged by size: 999 365 278 86 BseYI C'CCAG_C Cuts at: 0 781 910 1520 1728 Size: 781 129 610 208 Fragments arranged by size: 781 610 208 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 980 1728 Size: 980 748 BsiHKAI G_wGCw'C Cuts at: 0 997 1728 Size: 997 731 BsmBI CGTCTCn'nnnn_ Cuts at: 0 7 1728 Size: 7 1721 Bsp1286I G_dGCh'C Cuts at: 0 194 997 1728 Size: 194 803 731 Fragments arranged by size: 803 731 194 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1075 1563 1728 Size: 1075 488 165 Fragments arranged by size: 1075 488 165 BssHII G'CGCG_C Cuts at: 0 212 214 1728 Size: 212 2 1514 Fragments arranged by size: 1514 212 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1090 1728 Size: 1090 638 BstYI r'GATC_y Cuts at: 0 117 438 1728 Size: 117 321 1290 Fragments arranged by size: 1290 321 117 BtsI GCAGTG_nn' Cuts at: 0 626 1728 Size: 626 1102 EarI CTCTTCn'nnn_ Cuts at: 0 251 394 527 1041 1170 1187 1728 Size: 251 143 133 514 129 17 541 Fragments arranged by size: 541 514 251 143 133 129 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 124 1728 Size: 124 1604 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 135 202 1728 Size: 135 67 1526 Fragments arranged by size: 1526 135 67 EcoO109I rG'GnC_Cy Cuts at: 0 161 1728 Size: 161 1567 EcoRI G'AATT_C Cuts at: 0 276 1728 Size: 276 1452 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1216 1728 Size: 1216 512 HaeII r_GCGC'y Cuts at: 0 144 1728 Size: 144 1584 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 92 124 736 768 1438 1470 1728 Size: 92 32 612 32 670 32 258 Fragments arranged by size: 670 612 258 92 32 32 32 HincII GTy'rAC Cuts at: 0 90 1728 Size: 90 1638 HindIII A'AGCT_T Cuts at: 0 1438 1728 Size: 1438 290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1398 1728 Size: 1398 330 MspA1I CmG'CkG Cuts at: 0 1405 1728 Size: 1405 323 NspI r_CATG'y Cuts at: 0 1236 1728 Size: 1236 492 PasI CC'CwG_GG Cuts at: 0 1063 1728 Size: 1063 665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 633 665 717 749 1728 Size: 633 32 52 32 979 Fragments arranged by size: 979 633 52 32 32 PshAI GACnn'nnGTC Cuts at: 0 1382 1728 Size: 1382 346 PstI C_TGCA'G Cuts at: 0 873 1086 1728 Size: 873 213 642 Fragments arranged by size: 873 642 213 PvuII CAG'CTG Cuts at: 0 1405 1728 Size: 1405 323 SalI G'TCGA_C Cuts at: 0 88 1728 Size: 88 1640 SfcI C'TryA_G Cuts at: 0 281 869 1082 1380 1728 Size: 281 588 213 298 348 Fragments arranged by size: 588 348 298 281 213 SmlI C'TyrA_G Cuts at: 0 1412 1560 1728 Size: 1412 148 168 Fragments arranged by size: 1412 168 148 SphI G_CATG'C Cuts at: 0 1236 1728 Size: 1236 492 SspI AAT'ATT Cuts at: 0 382 1499 1728 Size: 382 1117 229 Fragments arranged by size: 1117 382 229 StuI AGG'CCT Cuts at: 0 173 1034 1728 Size: 173 861 694 Fragments arranged by size: 861 694 173 StyI C'CwwG_G Cuts at: 0 168 1728 Size: 168 1560 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 508 1383 1728 Size: 508 875 345 Fragments arranged by size: 875 508 345 TatI w'GTAC_w Cuts at: 0 612 1714 1728 Size: 612 1102 14 Fragments arranged by size: 1102 612 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 687 719 1728 Size: 687 32 1009 Fragments arranged by size: 1009 687 32 Enzymes that do cut: AarI AccI AcuI AfeI AlwNI ApoI AseI AvrII BanII BglII BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BssHII BstAPI BstYI BtsI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MmeI MspA1I NspI PasI PpiI PshAI PstI PvuII SalI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI