(Linear) (Six-base) MAPSORT of: KIEE4550.seq Check: 2985 from: 1 to: 1971 KIEE4550.orf With 182 enzymes: SgfI * March 22, 2012 18:21 .. AccI GT'mk_AC Cuts at: 0 58 954 1971 Size: 58 896 1017 Fragments arranged by size: 1017 896 58 Acc65I G'GTAC_C Cuts at: 0 117 1971 Size: 117 1854 AclI AA'CG_TT Cuts at: 0 212 1971 Size: 212 1759 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 6 93 272 629 706 717 812 1172 Size: 6 87 179 357 77 11 95 360 Cuts at: 1172 1631 1684 1971 Size: 459 53 287 Fragments arranged by size: 459 360 357 287 179 95 87 77 53 11 6 AflIII A'CryG_T Cuts at: 0 882 1971 Size: 882 1089 AleI CACnn'nnGTG Cuts at: 0 343 1971 Size: 343 1628 AlwNI CAG_nnn'CTG Cuts at: 0 1426 1848 1971 Size: 1426 422 123 Fragments arranged by size: 1426 422 123 ApoI r'AATT_y Cuts at: 0 709 898 1009 1289 1393 1971 Size: 709 189 111 280 104 578 Fragments arranged by size: 709 578 280 189 111 104 AseI AT'TA_AT Cuts at: 0 1901 1971 Size: 1901 70 AvaI C'yCGr_G Cuts at: 0 1255 1971 Size: 1255 716 AvrII C'CTAG_G Cuts at: 0 1266 1971 Size: 1266 705 BanI G'GyrC_C Cuts at: 0 117 1971 Size: 117 1854 BanII G_rGCy'C Cuts at: 0 1488 1971 Size: 1488 483 BbsI GAAGACnn'nnnn_ Cuts at: 0 301 453 1826 1971 Size: 301 152 1373 145 Fragments arranged by size: 1373 301 152 145 BlpI GC'TnA_GC Cuts at: 0 1838 1971 Size: 1838 133 BmtI G_CTAG'C Cuts at: 0 1588 1971 Size: 1588 383 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1529 1971 Size: 1529 442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 828 979 1971 Size: 766 62 151 992 Fragments arranged by size: 992 766 151 62 BsaAI yAC'GTr Cuts at: 0 412 1971 Size: 412 1559 BsaHI Gr'CG_yC Cuts at: 0 10 1971 Size: 10 1961 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 869 899 1971 Size: 869 30 1072 Fragments arranged by size: 1072 869 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 890 1321 1971 Size: 890 431 650 Fragments arranged by size: 890 650 431 BseYI C'CCAG_C Cuts at: 0 391 1740 1971 Size: 391 1349 231 Fragments arranged by size: 1349 391 231 BsiHKAI G_wGCw'C Cuts at: 0 679 1971 Size: 679 1292 Bsp1286I G_dGCh'C Cuts at: 0 679 1488 1971 Size: 679 809 483 Fragments arranged by size: 809 679 483 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 993 1971 Size: 993 978 BsrBI CCG'CTC Cuts at: 0 18 1971 Size: 18 1953 BsrDI GCAATG_nn' Cuts at: 0 426 754 1971 Size: 426 328 1217 Fragments arranged by size: 1217 426 328 BssSI C'ACGA_G Cuts at: 0 1167 1971 Size: 1167 804 BstXI CCAn_nnnn'nTGG Cuts at: 0 121 1740 1971 Size: 121 1619 231 Fragments arranged by size: 1619 231 121 BtsI GCAGTG_nn' Cuts at: 0 132 1971 Size: 132 1839 DraI TTT'AAA Cuts at: 0 1545 1971 Size: 1545 426 EaeI y'GGCC_r Cuts at: 0 488 1971 Size: 488 1483 EarI CTCTTCn'nnn_ Cuts at: 0 25 1237 1971 Size: 25 1212 734 Fragments arranged by size: 1212 734 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 6 93 272 629 706 717 812 1172 Size: 6 87 179 357 77 11 95 360 Cuts at: 1172 1631 1684 1971 Size: 459 53 287 Fragments arranged by size: 459 360 357 287 179 95 87 77 53 11 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 1706 1971 Size: 1706 265 EcoO109I rG'GnC_Cy Cuts at: 0 1276 1971 Size: 1276 695 EcoRI G'AATT_C Cuts at: 0 1393 1971 Size: 1393 578 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1030 1062 1749 1781 1971 Size: 1030 32 687 32 190 Fragments arranged by size: 1030 687 190 32 32 HincII GTy'rAC Cuts at: 0 955 1313 1971 Size: 955 358 658 Fragments arranged by size: 955 658 358 HindIII A'AGCT_T Cuts at: 0 158 1971 Size: 158 1813 HpaI GTT'AAC Cuts at: 0 1313 1971 Size: 1313 658 KpnI G_GTAC'C Cuts at: 0 121 1971 Size: 121 1850 MfeI C'AATT_G Cuts at: 0 686 1971 Size: 686 1285 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 31 691 1459 1971 Size: 31 660 768 512 Fragments arranged by size: 768 660 512 31 MscI TGG'CCA Cuts at: 0 490 1971 Size: 490 1481 MslI CAynn'nnrTG Cuts at: 0 343 1828 1971 Size: 343 1485 143 Fragments arranged by size: 1485 343 143 NheI G'CTAG_C Cuts at: 0 1584 1971 Size: 1584 387 NspI r_CATG'y Cuts at: 0 886 1380 1971 Size: 886 494 591 Fragments arranged by size: 886 591 494 PasI CC'CwG_GG Cuts at: 0 591 1971 Size: 591 1380 PciI A'CATG_T Cuts at: 0 882 1971 Size: 882 1089 PpuMI rG'GwC_Cy Cuts at: 0 1276 1971 Size: 1276 695 PsiI TTA'TAA Cuts at: 0 190 821 1971 Size: 190 631 1150 Fragments arranged by size: 1150 631 190 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1200 1232 1971 Size: 1200 32 739 Fragments arranged by size: 1200 739 32 SalI G'TCGA_C Cuts at: 0 953 1971 Size: 953 1018 SapI GCTCTTCn'nnn_ Cuts at: 0 25 1971 Size: 25 1946 SmlI C'TyrA_G Cuts at: 0 781 843 994 1971 Size: 781 62 151 977 Fragments arranged by size: 977 781 151 62 SnaBI TAC'GTA Cuts at: 0 412 1971 Size: 412 1559 SspI AAT'ATT Cuts at: 0 335 1971 Size: 335 1636 StuI AGG'CCT Cuts at: 0 70 841 1971 Size: 70 771 1130 Fragments arranged by size: 1130 771 70 StyI C'CwwG_G Cuts at: 0 1266 1589 1971 Size: 1266 323 382 Fragments arranged by size: 1266 382 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1732 1971 Size: 1732 239 XbaI T'CTAG_A Cuts at: 0 174 1971 Size: 174 1797 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlwNI ApoI AseI AvaI AvrII BanI BanII BbsI BlpI BmtI BplI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstXI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI MslI NheI NspI PasI PciI PpuMI PsiI PsrI SalI SapI SmlI SnaBI SspI StuI StyI TaqII XbaI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII KasI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SwaI TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI