################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MboI MfeI MflI MluI MroI MunI MvnI NaeI NarI NdeII NgoMIV NheI NotI NruI NspI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### ApaI 5 GGGCCC GGGCC^C Asp700I 245 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 757 GGACC G^G[AT]CC BalI 732 TGGCCA TGG^CCA BanII 5 GGGCCC G[GA]GC[CT]^C BbsI 967 GTCTTC GAAGAC(2/6) BbvCI 283 CCTCAGC CCTCAGC(-5/-2) BglI 282 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 523 GCTGAGC GC^T[ACGT]AGC BmyI 5 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 523 GCTGAGC GC^T[ACGT]AGC BpuAI 967 GTCTTC GAAGAC(2/6) BsgI 303 GTGCAG GTGCAG(16/14) BsmAI 702 GTCTC GTCTC(1/5) BsmFI 406 GGGAC GGGAC(10/14) BsmI 861 GAATGC GAATGC(1/-1) Bsp1286I 5 GGGCCC G[AGT]GC[ACT]^C BspMI 56 ACCTGC ACCTGC(4/8) BsrDI 603 CATTGC GCAATG(2/0) BstBI 909 TTCGAA TT^CGAA CelII 523 GCTGAGC GC^T[ACGT]AGC DraII 4 GGGGCCC [GA]G^G[ACGT]CC[CT] EaeI 732 TGGCCA [CT]^GGCC[GA] EcoNI 463 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoT22I 454 ATGCAT ATGCA^T FauI 37 GCGGG CCCGC(4/6) HgaI 185 GACGC GACGC(5/10) MaeII 413 ACGT A^CGT MaeIII 368 GTAAC ^GT[ACGT]AC MluNI 732 TGGCCA TGG^CCA MscI 732 TGGCCA TGG^CCA NdeI 764 CATATG CA^TATG NsiI 454 ATGCAT ATGCA^T NspV 909 TTCGAA TT^CGAA PacI 1033 TTAATTAA TTAAT^TAA PflMI 781 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 104 GCATC GCATC(5/9) SfuI 909 TTCGAA TT^CGAA SmaI 8 CCCGGG CCC^GGG SspI 993 AATATT AAT^ATT Van91I 781 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 8 CCCGGG C^CCGGG XmaI 8 CCCGGG C^CCGGG XmnI 245 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 53 GGTACC G^GTACC 598 GGTACC AcsI 214 AAATTC [GA]^AATT[CT] 223 AAATTT ApoI 214 AAATTC [GA]^AATT[CT] 223 AAATTT Asp718I 53 GGTACC G^GTACC 598 GGTACC AvaI 8 CCCGGG C^[CT]CG[GA]G 112 CTCGGG BcgI 104 GCATCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 901 GCAGCAACTTCG BfaI 532 CTAG C^TAG 946 CTAG BsoBI 8 CCCGGG C^[CT]CG[GA]G 112 CTCGGG BsrI 333 ACTGG ACTGG(1/-1) 685 ACTGG CfoI 14 GCGC GCG^C 348 GCGC EarI 275 CTCTTC CTCTTC(1/4) 376 CTCTTC EcoT14I 695 CCATGG C^C[AT][AT]GG 875 CCATGG HaeII 13 GGCGCT [GA]GCGC^[CT] 347 GGCGCT HapII 9 CCGG C^CGG 65 CCGG HhaI 14 GCGC GCG^C 348 GCGC HinP1I 14 GCGC G^CGC 348 GCGC HinfI 138 GAATC G^A[ACGT]TC 838 GAATC HpaII 9 CCGG C^CGG 65 CCGG HphI 208 GGTGA GGTGA(8/7) 872 TCACC KpnI 53 GGTACC GGTAC^C 598 GGTACC Ksp632I 275 CTCTTC CTCTTC(1/4) 376 CTCTTC MaeI 532 CTAG C^TAG 946 CTAG MseI 1033 TTAA T^TAA 1037 TTAA MslI 748 CATATCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 876 CATGGGGATG MspI 9 CCGG C^CGG 65 CCGG NcoI 695 CCATGG C^CATGG 875 CCATGG PvuII 163 CAGCTG CAG^CTG 521 CAGCTG SapI 274 GCTCTTC GCTCTTC(1/4) 375 GCTCTTC StyI 695 CCATGG C^C[AT][AT]GG 875 CCATGG TaqI 243 TCGA T^CGA 910 TCGA TfiI 138 GAATC G^A[AT]TC 838 GAATC Tru9I 1033 TTAA T^TAA 1037 TTAA TspRI 270 CACTG CA[GC]TG[ACGT][ACGT]^ 292 CAGTG TthHB8I 243 TCGA T^CGA 910 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI BanI BbvI BcnI BsaJI BsiYI BslI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DsaI EcoRII Fnu4HI FokI HaeIII ItaI MboII MnlI MspA1I MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp509I