(Circular) (Six-base) MAPSORT of: pFN21AE6303.seq Check: 6014 from: 1 to: 5746 pFN21AE6303 5746 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 2058 2603 3067 2058 Size: 545 464 4737 Fragments arranged by size: 4737 545 464 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2371 2965 3809 5193 5461 1632 Size: 635 104 594 844 1384 268 1917 Fragments arranged by size: 1917 1384 844 635 594 268 104 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaI G_GGCC'C Cuts at: 2014 2014 Size: 5746 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 2219 2228 3055 3281 3433 5542 1493 Size: 726 9 827 226 152 2109 1697 Fragments arranged by size: 2109 1697 827 726 226 152 9 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2117 3071 1870 Size: 73 70 104 954 4545 Fragments arranged by size: 4545 954 104 73 70 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2587 2620 2587 Size: 33 5713 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2058 2239 2603 3067 4522 Size: 325 217 627 271 181 364 464 1455 Cuts at: 4522 618 Size: 1842 Fragments arranged by size: 1842 1455 627 464 364 325 271 217 181 BanII G_rGCy'C Cuts at: 1391 2014 3065 1391 Size: 623 1051 4072 Fragments arranged by size: 4072 1051 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2965 928 Size: 971 1066 3709 Fragments arranged by size: 3709 1066 971 BbvCI CC'TCA_GC Cuts at: 2289 2289 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2098 2132 2895 2929 3937 3971 1903 Size: 34 161 34 763 34 1008 34 3678 Fragments arranged by size: 3678 1008 763 161 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BfrBI ATG'CAT Cuts at: 2461 2461 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2293 4357 Size: 107 122 71 631 605 337 284 2064 Cuts at: 4357 136 Size: 1525 Fragments arranged by size: 2064 1525 631 605 337 284 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 2529 3163 2529 Size: 634 5112 Bme1580I G_kGCm'C Cuts at: 1790 2014 3807 4963 1790 Size: 224 1793 1156 2573 Fragments arranged by size: 2573 1793 1156 224 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4406 1239 Size: 3167 2579 Bpu10I CC'TnA_GC Cuts at: 2289 2289 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5746 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2329 1163 Size: 573 593 4580 Fragments arranged by size: 4580 593 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3807 3892 4963 3807 Size: 85 1071 4590 Fragments arranged by size: 4590 1071 85 BsmI GAATG_Cn' Cuts at: 2872 3257 3350 2872 Size: 385 93 5268 Fragments arranged by size: 5268 385 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 3065 3807 3892 4963 1391 Size: 399 224 1051 742 85 1071 2174 Fragments arranged by size: 2174 1071 1051 742 399 224 85 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 3101 844 Size: 1226 1031 3489 Fragments arranged by size: 3489 1226 1031 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 4241 4415 65 Size: 1183 1359 1634 174 1396 Fragments arranged by size: 1634 1396 1359 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 2915 4550 2915 Size: 1635 4111 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2516 2614 2759 2887 2516 Size: 98 145 128 5375 Fragments arranged by size: 5375 145 128 98 BstYI r'GATC_y Cuts at: 1972 3076 3443 3828 3845 5297 5383 5395 5741 Size: 1104 367 385 17 1452 86 12 346 Cuts at: 5741 1972 Size: 1977 Fragments arranged by size: 1977 1452 1104 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2700 2758 2880 3016 513 Size: 552 939 696 58 122 136 3243 Fragments arranged by size: 3243 939 696 552 136 122 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2937 487 Size: 683 56 1711 3296 Fragments arranged by size: 3296 1711 683 56 BtsI GCAGTG_nn' Cuts at: 2274 2303 3262 4056 4076 2274 Size: 29 959 794 20 3944 Fragments arranged by size: 3944 959 794 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2737 3193 4082 Size: 54 1425 50 275 18 907 456 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 907 889 456 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2387 3676 1353 Size: 933 101 1289 3423 Fragments arranged by size: 3423 1289 933 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2371 2965 3809 4406 5193 5461 Size: 393 635 104 594 844 597 787 268 Cuts at: 5461 1239 Size: 1524 Fragments arranged by size: 1524 844 787 635 597 594 393 268 104 EcoNI CCTnn'n_nnAGG Cuts at: 2472 2472 Size: 5746 EcoO109I rG'GnC_Cy Cuts at: 2010 2010 Size: 5746 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5746 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 2022 2356 4893 1678 Size: 344 334 2537 2531 Fragments arranged by size: 2537 2531 344 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 4387 4419 4461 Size: 32 135 32 609 32 2174 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 2174 609 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2062 2607 3071 2062 Size: 545 464 4737 Fragments arranged by size: 4737 545 464 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2617 2908 4860 5044 1305 Size: 1312 291 1952 184 2007 Fragments arranged by size: 2007 1952 1312 291 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2739 10 Size: 54 1425 343 907 3017 Fragments arranged by size: 3017 1425 907 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2757 2885 3704 4063 4222 518 Size: 648 1591 128 819 359 159 2042 Fragments arranged by size: 2042 1591 819 648 359 159 128 MspA1I CmG'CkG Cuts at: 1606 2170 2293 2528 3162 3839 4987 5232 1606 Size: 564 123 235 634 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 634 564 245 235 123 NcoI C'CATG_G Cuts at: 513 1065 2004 2700 2880 513 Size: 552 939 696 180 3379 Fragments arranged by size: 3379 939 696 552 180 NdeI CA'TA_TG Cuts at: 387 2770 387 Size: 2383 3363 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NsiI A_TGCA'T Cuts at: 2463 2463 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PacI TTA_AT'TAA Cuts at: 3042 3042 Size: 5746 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 2792 1108 Size: 1684 4062 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 2234 3316 2234 Size: 1082 4664 PspOMI G'GGCC_C Cuts at: 2010 2010 Size: 5746 PstI C_TGCA'G Cuts at: 830 3098 830 Size: 2268 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2170 2528 2170 Size: 358 5388 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 2387 2286 Size: 101 5645 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 3094 4229 4910 5101 826 Size: 219 2049 1135 681 191 1471 Fragments arranged by size: 2049 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2015 3073 1872 Size: 143 1058 4545 Fragments arranged by size: 4545 1058 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3000 3670 5 Size: 47 2948 670 2081 Fragments arranged by size: 2948 2081 670 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2700 2880 3185 513 Size: 552 939 696 180 305 3074 Fragments arranged by size: 3074 939 696 552 305 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2605 3773 3790 3943 4128 4573 325 Size: 1255 1025 1168 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1168 1025 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3992 5704 96 Size: 274 80 33 51 1443 2015 1712 138 Fragments arranged by size: 2015 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5706 5738 5706 Size: 32 5714 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2013 3071 1870 Size: 143 1058 4545 Fragments arranged by size: 4545 1058 143 XmnI GAAnn'nnTTC Cuts at: 2254 3875 2254 Size: 1621 4125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI