Restriction map for
pFN21AE5303
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AclI 200 AACGTT AA^CGTT
AcsI 520 AAATTT [GA]^AATT[CT]
AfaI 360 GTAC GT^AC
AlwI 451 GATCC GGATC(4/5)
AlwNI 449 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 141 CCTCAGG CC^T[ACGT]AGG
ApaI 9 GGGCCC GGGCC^C
ApoI 520 AAATTT [GA]^AATT[CT]
Asp700I 118 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 5 CCCGGG C^[CT]CG[GA]G
AvaII 100 GGTCC G^G[AT]CC
BalI 767 TGGCCA TGG^CCA
BanII 9 GGGCCC G[GA]GC[CT]^C
BbvCI 111 GCTGAGG CCTCAGC(-5/-2)
BbvI 37 GCAGC GCAGC(8/12)
BclI 581 TGATCA T^GATCA
BmyI 9 GGGCCC G[AGT]GC[ACT]^C
BpmI 170 CTGGAG CTGGAG(16/14)
BsgI 478 GTGCAG GTGCAG(16/14)
BsiEI 567 CGGTCG CG[GA][CT]^CG
BsmI 728 GCATTC GAATGC(1/-1)
BsoBI 5 CCCGGG C^[CT]CG[GA]G
Bsp1286I 9 GGGCCC G[AGT]GC[ACT]^C
BspHI 423 TCATGA T^CATGA
BsrDI 686 GCAATG GCAATG(2/0)
BssSI 657 CACGAG CACGAG(-5/-1)
BstAPI 48 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 450 AGATCC [GA]^GATC[CT]
Bsu36I 141 CCTCAGG CC^T[ACGT]AGG
Cac8I 765 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 40 GCGC GCG^C
DraIII 412 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 767 TGGCCA [CT]^GGCC[GA]
Eco81I 141 CCTCAGG CC^T[ACGT]AGG
FbaI 581 TGATCA T^GATCA
Fnu4HI 37 GCAGC GC^[ACGT]GC
HaeII 39 AGCGCC [GA]GCGC^[CT]
HapII 6 CCGG C^CGG
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HincII 165 GTTGAC GT[CT]^[GA]AC
HindII 165 GTTGAC GT[CT]^[GA]AC
HpaII 6 CCGG C^CGG
ItaI 37 GCAGC GC^[ACGT]GC
MaeII 201 ACGT A^CGT
MflI 450 AGATCC [GA]^GATC[CT]
MluNI 767 TGGCCA TGG^CCA
MscI 767 TGGCCA TGG^CCA
MseI 25 TTAA T^TAA
MspI 6 CCGG C^CGG
NspI 624 GCATGT [GA]CATG^[CT]
PpuMI 99 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 200 AACGTT AA^CGTT
RcaI 423 TCATGA T^CATGA
RsaI 360 GTAC GT^AC
SexAI 413 ACCTGGT A^CC[AT]GGT
SmaI 5 CCCGGG CCC^GGG
SmlI 154 CTCAAG C^T[CT][GA]AG
Tru9I 25 TTAA T^TAA
TseI 37 GCAGC G^C[AT]GC
XhoII 450 AGATCC [GA]^GATC[CT]
XmaCI 5 CCCGGG C^CCGGG
XmaI 5 CCCGGG C^CCGGG
XmnI 118 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 110 AGCT AG^CT
649 AGCT
BcnI 5 CCCGG CC^[GC]GG
6 CCGGG
BsmFI 493 GGGAC GGGAC(10/14)
101 GTCCC
BsrI 169 ACTGG ACTGG(1/-1)
496 ACTGG
DdeI 112 CTGAG C^T[ACGT]AG
142 CTCAG
EarI 349 GAAGAG CTCTTC(1/4)
748 GAAGAG
EcoT14I 314 CCAAGG C^C[AT][AT]GG
700 CCTTGG
Ksp632I 349 GAAGAG CTCTTC(1/4)
748 GAAGAG
MaeIII 238 GTCAC ^GT[ACGT]AC
418 GTGAC
MslI 339 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
459 CATCAAGGTG
MwoI 21 GCACTTAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
48 GCATTGGATGC
NciI 5 CCCGG CC^[GC]GG
6 CCGGG
PleI 152 GACTC GAGTC(4/5)
420 GACTC
StyI 314 CCAAGG C^C[AT][AT]GG
700 CCTTGG
TfiI 254 GATTC G^A[AT]TC
404 GAATC
Tsp45I 238 GTCAC ^GT[GC]AC
418 GTGAC
TspRI 344 CAGTG CA[GC]TG[ACGT][ACGT]^
783 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI BbsI BpuAI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DpnII DraII EcoO109I EcoRII FokI
HaeIII HinfI HphI MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
Tsp509I TthHB8I