################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspHI BsrBI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I EarI Eco47III Eco81I EcoRV EcoT22I FbaI FseI FspI HindIII KasI KpnI Ksp632I MamI MfeI MluI MroI MunI NarI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SexAI SfiI SfoI SfuI SgrAI SnaBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1710 GACGTC GACGT^C Alw44I 266 GTGCAC G^TGCAC ApaLI 266 GTGCAC G^TGCAC BanII 1554 GAGCCC G[GA]GC[CT]^C BglII 1976 AGATCT A^GATCT BsaI 1018 GGTCTC GGTCTC(1/5) BsaWI 80 ACCGGA [AT]^CCGG[AT] BsiEI 1220 CGGCCG CG[GA][CT]^CG BsmI 357 GAATGC GAATGC(1/-1) Bsp1407I 1496 TGTACA T^GTACA BspLU11I 1187 ACATGT A^CATGT BsrDI 2036 GCAATG GCAATG(2/0) BsrGI 1496 TGTACA T^GTACA BssSI 1485 CACGAG CACGAG(-5/-1) BstEII 339 GGTGACC G^GT[ACGT]ACC BstPI 339 GGTGACC G^GT[ACGT]ACC DraIII 178 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1909 GACAACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1220 CGGCCG C^GGCCG EclXI 1220 CGGCCG C^GGCCG Eco52I 1220 CGGCCG C^GGCCG EcoNI 1932 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 339 GGTGACC G^GT[ACGT]ACC EcoRI 94 GAATTC G^AATTC HincII 1051 GTTAAC GT[CT]^[GA]AC HindII 1051 GTTAAC GT[CT]^[GA]AC HpaI 1051 GTTAAC GTT^AAC KspI 1217 CCGCGG CCGC^GG NdeI 203 CATATG CA^TATG NotI 1219 GCGGCCGC GC^GGCCGC PflMI 1886 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacII 1217 CCGCGG CCGC^GG SbfI 105 CCTGCAGG CCTGCA^GG SmaI 803 CCCGGG CCC^GGG SmlI 1071 CTTGAG C^T[CT][GA]AG SpeI 1821 ACTAGT A^CTAGT SphI 1419 GCATGC GCATG^C Sse8387I 105 CCTGCAGG CCTGCA^GG SspBI 1496 TGTACA T^GTACA StuI 1005 AGGCCT AGG^CCT Van91I 1886 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 803 CCCGGG C^CCGGG XmaI 803 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 1264 GATCC GGATC(4/5) 1803 GGATC BalI 405 TGGCCA TGG^CCA 1739 TGGCCA BbrPI 1492 CACGTG CAC^GTG 2053 CACGTG BlpI 388 GCTCAGC GC^T[ACGT]AGC 2226 GCTTAGC Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC 2226 GCTTAGC BsaAI 1492 CACGTG [CT]AC^GT[GA] 2053 CACGTG BseRI 873 CTCCTC GAGGAG(10/8) 2161 CTCCTC BsrFI 14 GCCGGC [GA]^CCGG[CT] 484 GCCGGC BstAPI 375 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 784 GCAAGCCCTGC BstYI 1803 GGATCT [GA]^GATC[CT] 1976 AGATCT CelII 388 GCTCAGC GC^T[ACGT]AGC 2226 GCTTAGC Cfr10I 14 GCCGGC [GA]^CCGG[CT] 484 GCCGGC MboII 772 TCTTC GAAGA(8/7) 1979 TCTTC MflI 1803 GGATCT [GA]^GATC[CT] 1976 AGATCT MluNI 405 TGGCCA TGG^CCA 1739 TGGCCA MscI 405 TGGCCA TGG^CCA 1739 TGGCCA NaeI 14 GCCGGC GCC^GGC 484 GCCGGC NcoI 344 CCATGG C^CATGG 1250 CCATGG NgoMIV 14 GCCGGC G^CCGGC 484 GCCGGC PmaCI 1492 CACGTG CAC^GTG 2053 CACGTG PmlI 1492 CACGTG CAC^GTG 2053 CACGTG ScaI 1654 AGTACT AGT^ACT 2143 AGTACT TaqI 818 TCGA T^CGA 1571 TCGA TthHB8I 818 TCGA T^CGA 1571 TCGA XcmI 1056 CCAAGCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1664 CCATTTGGGACCTGG XhoII 1803 GGATCT [GA]^GATC[CT] 1976 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwNI ApoI AspHI AvaI AvaII BanI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI