(Circular) (Six-base) MAPSORT of: pFN21AB6266.seq Check: 1967 from: 1 to: 7015 pFN21AB6266 7015 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4098 4098 Size: 7015 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3719 4825 278 Size: 53 83 186 936 2183 1106 2468 Fragments arranged by size: 2468 2183 1106 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4358 1937 Size: 2421 4594 Acc65I G'GTAC_C Cuts at: 4336 4336 Size: 7015 AclI AA'CG_TT Cuts at: 1563 5142 5515 1563 Size: 3579 373 3063 Fragments arranged by size: 3579 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 5078 6462 6730 1632 Size: 806 2640 1384 268 1917 Fragments arranged by size: 2640 1917 1384 806 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6818 AflIII A'CryG_T Cuts at: 1203 3192 3496 3498 5914 6891 1203 Size: 1989 304 2 2416 977 1327 Fragments arranged by size: 2416 1989 1327 977 304 2 AgeI A'CCGG_T Cuts at: 5846 5846 Size: 7015 AhdI GACnn_n'nnGTC Cuts at: 5744 5744 Size: 7015 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7015 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2058 2580 1184 Size: 874 522 5619 Fragments arranged by size: 5619 874 522 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 2409 2441 2090 Size: 32 287 32 6664 Fragments arranged by size: 6664 287 32 32 AlwNI CAG_nnn'CTG Cuts at: 2110 3170 3930 4758 6330 2110 Size: 1060 760 828 1572 2795 Fragments arranged by size: 2795 1572 1060 828 760 ApaLI G'TGCA_C Cuts at: 2271 5072 6228 2271 Size: 2801 1156 3058 Fragments arranged by size: 3058 2801 1156 ApoI r'AATT_y Cuts at: 1493 2099 2660 4118 4324 4550 4702 6811 1493 Size: 606 561 1458 206 226 152 2109 1697 Fragments arranged by size: 2109 1697 1458 606 561 226 206 152 AseI AT'TA_AT Cuts at: 160 1032 5569 160 Size: 872 4537 1606 Fragments arranged by size: 4537 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 AvaI C'yCGr_G Cuts at: 1870 1943 2477 2808 4276 4340 1870 Size: 73 534 331 1468 64 4545 Fragments arranged by size: 4545 1468 534 331 73 64 BamHI G'GATC_C Cuts at: 4345 4712 4345 Size: 367 6648 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2022 2993 3887 4336 5791 Size: 325 217 627 235 971 894 449 1455 Cuts at: 5791 618 Size: 1842 Fragments arranged by size: 1842 1455 971 894 627 449 325 235 217 BanII G_rGCy'C Cuts at: 1391 3563 4334 1391 Size: 2172 771 4072 Fragments arranged by size: 4072 2172 771 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6044 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5206 5240 1903 Size: 34 3269 34 3678 Fragments arranged by size: 3678 3269 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4907 6117 4907 Size: 1210 5805 BclI T'GATC_A Cuts at: 1548 1548 Size: 7015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2882 3224 3236 Size: 107 122 71 631 605 1210 342 12 Cuts at: 3236 3549 5626 136 Size: 313 2077 1525 Fragments arranged by size: 2077 1525 1210 631 605 342 313 122 107 71 12 BglII A'GATC_T Cuts at: 3981 7010 3981 Size: 3029 3986 BlpI GC'TnA_GC Cuts at: 2394 4232 4432 2394 Size: 1838 200 4977 Fragments arranged by size: 4977 1838 200 Bme1580I G_kGCm'C Cuts at: 1790 2275 2597 2996 3099 5076 6232 1790 Size: 485 322 399 103 1977 1156 2573 Fragments arranged by size: 2573 1977 1156 485 399 322 103 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7015 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2417 4746 5704 Size: 416 53 83 399 24 1000 2329 958 Cuts at: 5704 442 Size: 1753 Fragments arranged by size: 2329 1753 1000 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7015 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2689 2991 3021 5675 1239 Size: 1450 302 30 2654 2579 Fragments arranged by size: 2654 2579 1450 302 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3097 5140 6005 6303 3097 Size: 2043 865 298 3809 Fragments arranged by size: 3809 2043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3029 5678 882 Size: 617 1530 2649 2219 Fragments arranged by size: 2649 2219 1530 617 BsaAI yAC'GTr Cuts at: 493 1204 3499 4060 4806 493 Size: 711 2295 561 746 2702 Fragments arranged by size: 2702 2295 746 711 561 BsaBI GATnn'nnATC Cuts at: 7009 7009 Size: 7015 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 3396 3716 4822 Size: 53 83 186 936 497 1366 320 1106 Cuts at: 4822 5204 5839 275 Size: 382 635 1451 Fragments arranged by size: 1451 1366 1106 936 635 497 382 320 186 83 53 BsaWI w'CCGG_w Cuts at: 2085 5448 5846 6120 6267 6678 2085 Size: 3363 398 274 147 411 2422 Fragments arranged by size: 3363 2422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3233 3263 3286 3316 3449 Size: 30 165 30 1830 30 23 30 133 Cuts at: 3449 3479 3701 3731 3959 3989 1178 Size: 30 222 30 228 30 4204 Fragments arranged by size: 4204 1830 228 222 165 133 30 30 30 30 30 30 30 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2869 4157 1186 Size: 1683 1288 4044 Fragments arranged by size: 4044 1683 1288 BseYI C'CCAG_C Cuts at: 3570 6218 6714 3570 Size: 2648 496 3871 Fragments arranged by size: 3871 2648 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3616 3797 4040 4070 1163 Size: 573 1880 181 243 30 4108 Fragments arranged by size: 4108 1880 573 243 181 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3228 4465 5226 6254 1540 Size: 275 186 1227 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2275 2515 2574 5076 5161 6232 2275 Size: 240 59 2502 85 1071 3058 Fragments arranged by size: 3058 2502 1071 240 85 59 BsmI GAATG_Cn' Cuts at: 2368 4526 4619 2368 Size: 2158 93 4764 Fragments arranged by size: 4764 2158 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2515 2574 2597 2996 3099 3563 Size: 399 485 240 59 23 399 103 464 Cuts at: 3563 4334 5076 5161 6232 1391 Size: 771 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 485 464 399 399 240 103 85 59 23 BspEI T'CCGG_A Cuts at: 6678 6678 Size: 7015 BspHI T'CATG_A Cuts at: 4904 6634 4904 Size: 1730 5285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3343 3945 4098 4370 844 Size: 2499 602 153 272 3489 Fragments arranged by size: 3489 2499 602 272 153 BsrBI CCG'CTC Cuts at: 1394 1823 4902 1394 Size: 429 3079 3507 Fragments arranged by size: 3507 3079 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4048 5510 5684 65 Size: 1183 2800 1462 174 1396 Fragments arranged by size: 2800 1462 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 2489 5659 5846 1700 Size: 110 209 470 3170 187 2869 Fragments arranged by size: 3170 2869 470 209 187 110 BsrGI T'GTAC_A Cuts at: 96 3501 96 Size: 3405 3610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7015 BssSI C'ACGA_G Cuts at: 3490 5075 6087 3490 Size: 1585 1012 4418 Fragments arranged by size: 4418 1585 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 2795 794 Size: 1592 409 5014 Fragments arranged by size: 5014 1592 409 BstBI TT'CG_AA Cuts at: 5819 5819 Size: 7015 BstEII G'GTnAC_C Cuts at: 1184 2344 1184 Size: 1160 5855 BstYI r'GATC_y Cuts at: 1972 3808 3981 4345 4712 5097 5114 6566 6652 Size: 1836 173 364 367 385 17 1452 86 Cuts at: 6652 6664 7010 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1836 1452 385 367 364 346 173 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2349 2413 2871 3222 3231 3255 3451 Size: 552 1284 64 458 351 9 24 196 Cuts at: 3451 513 Size: 4077 Fragments arranged by size: 4077 1284 552 458 351 196 64 24 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2751 3781 487 Size: 683 56 1525 1030 3721 Fragments arranged by size: 3721 1525 1030 683 56 BtsI GCAGTG_nn' Cuts at: 2491 3099 4029 4531 5325 5345 2491 Size: 608 930 502 794 20 4161 Fragments arranged by size: 4161 930 794 608 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7015 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6980 DraI TTT'AAA Cuts at: 4319 4319 Size: 7015 DraIII CAC_nnn'GTG Cuts at: 2188 2188 Size: 7015 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3920 6022 809 Size: 507 2604 2102 1802 Fragments arranged by size: 2604 2102 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2410 3225 3744 Size: 54 1425 50 275 18 580 815 519 Cuts at: 3744 4462 5351 6980 8 Size: 718 889 1629 43 Fragments arranged by size: 1629 1425 889 815 718 580 519 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3225 4462 1537 Size: 275 1413 1237 4090 Fragments arranged by size: 4090 1413 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4945 1353 Size: 3592 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3722 4195 5602 5976 6122 387 Size: 1064 138 2133 473 1407 374 146 1280 Fragments arranged by size: 2133 1407 1280 1064 473 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2689 2991 3021 5078 5675 6462 Size: 393 806 251 302 30 2057 597 787 Cuts at: 6462 6730 1239 Size: 268 1524 Fragments arranged by size: 2057 1524 806 787 597 393 302 268 251 30 EcoNI CCTnn'n_nnAGG Cuts at: 3941 3941 Size: 7015 EcoO109I rG'GnC_Cy Cuts at: 2770 3676 3927 3934 2770 Size: 906 251 7 5851 Fragments arranged by size: 5851 906 251 7 EcoRI G'AATT_C Cuts at: 2099 4324 4702 2099 Size: 2225 378 4412 Fragments arranged by size: 4412 2225 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2910 3787 2910 Size: 877 6138 FspI TGC'GCA Cuts at: 5521 5521 Size: 7015 HaeII r_GCGC'y Cuts at: 1678 3003 3171 3213 3250 6162 1678 Size: 1325 168 42 37 2912 2531 Fragments arranged by size: 2912 2531 1325 168 42 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2687 2719 3399 3431 3701 Size: 32 135 32 1115 32 680 32 270 Cuts at: 3701 3733 3959 3991 4208 4240 5656 5688 5730 Size: 32 226 32 217 32 1416 32 42 Cuts at: 5730 5762 1373 Size: 32 2626 Fragments arranged by size: 2626 1416 1115 680 270 226 217 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3058 4359 4605 669 Size: 988 281 1120 1301 246 3079 Fragments arranged by size: 3079 1301 1120 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7015 HpaI GTT'AAC Cuts at: 3058 4605 3058 Size: 1547 5468 KpnI G_GTAC'C Cuts at: 4340 4340 Size: 7015 MfeI C'AATT_G Cuts at: 4614 4614 Size: 7015 MluI A'CGCG_T Cuts at: 6891 6891 Size: 7015 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3713 6129 6313 1305 Size: 2408 2416 184 2007 Fragments arranged by size: 2416 2408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2412 3746 10 Size: 54 1425 343 580 1334 3279 Fragments arranged by size: 3279 1425 1334 580 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2562 3800 4092 4247 4973 5332 Size: 648 1059 337 1238 292 155 726 359 Cuts at: 5332 5491 518 Size: 159 2042 Fragments arranged by size: 2042 1238 1059 726 648 359 337 292 159 155 MspA1I CmG'CkG Cuts at: 1606 3071 3224 3864 3955 4084 4431 5108 6256 Size: 1465 153 640 91 129 347 677 1148 Cuts at: 6256 6501 1606 Size: 245 2120 Fragments arranged by size: 2120 1465 1148 677 640 347 245 153 129 91 NaeI GCC'GGC Cuts at: 2021 2491 2021 Size: 470 6545 NcoI C'CATG_G Cuts at: 513 1065 2349 3255 513 Size: 552 1284 906 4273 Fragments arranged by size: 4273 1284 906 552 NdeI CA'TA_TG Cuts at: 387 2209 387 Size: 1822 5193 NgoMIV G'CCGG_C Cuts at: 2019 2489 2019 Size: 470 6545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7015 NotI GC'GGCC_GC Cuts at: 3225 4462 3225 Size: 1237 5778 NspI r_CATG'y Cuts at: 1132 1229 2983 3196 3428 4373 5918 1132 Size: 97 1754 213 232 945 1545 2229 Fragments arranged by size: 2229 1754 1545 945 232 213 97 PasI CC'CwG_GG Cuts at: 1348 3323 3991 1348 Size: 1975 668 4372 Fragments arranged by size: 4372 1975 668 PciI A'CATG_T Cuts at: 3192 5914 3192 Size: 2722 4293 PflMI CCAn_nnn'nTGG Cuts at: 1108 3897 1108 Size: 2789 4226 PfoI T'CCnGG_A Cuts at: 2070 2461 2070 Size: 391 6624 PmeI GTTT'AAAC Cuts at: 4319 4319 Size: 7015 PmlI CAC'GTG Cuts at: 3499 4060 3499 Size: 561 6454 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5084 5116 6597 6629 5084 Size: 32 1481 32 5470 Fragments arranged by size: 5470 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2770 3676 3927 3934 2770 Size: 906 251 7 5851 Fragments arranged by size: 5851 906 251 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7015 PsiI TTA'TAA Cuts at: 4585 4585 Size: 7015 PstI C_TGCA'G Cuts at: 830 2047 2115 3361 3785 3960 4367 830 Size: 1217 68 1246 424 175 407 3478 Fragments arranged by size: 3478 1246 1217 424 407 175 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7015 PvuII CAG'CTG Cuts at: 3071 3864 3955 4084 3071 Size: 793 91 129 6002 Fragments arranged by size: 6002 793 129 91 SacII CC_GC'GG Cuts at: 3225 3225 Size: 7015 SalI G'TCGA_C Cuts at: 1936 4357 1936 Size: 2421 4594 SbfI CC_TGCA'GG Cuts at: 2115 4367 2115 Size: 2252 4763 ScaI AGT'ACT Cuts at: 3661 4150 5263 6975 3661 Size: 489 1113 1712 3701 Fragments arranged by size: 3701 1712 1113 489 SfcI C'TryA_G Cuts at: 826 1045 2043 2111 3357 3781 3956 4293 4363 Size: 219 998 68 1246 424 175 337 70 Cuts at: 4363 5498 6179 6370 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1246 1135 998 681 424 337 219 191 175 70 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7015 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7015 SmaI CCC'GGG Cuts at: 1872 2810 4342 1872 Size: 938 1532 4545 Fragments arranged by size: 4545 1532 938 SmlI C'TyrA_G Cuts at: 820 1017 1943 3076 5119 6020 6282 820 Size: 197 926 1133 2043 901 262 1553 Fragments arranged by size: 2043 1553 1133 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4806 493 Size: 4313 2702 SpeI A'CTAG_T Cuts at: 152 3826 152 Size: 3674 3341 SphI G_CATG'C Cuts at: 1132 3428 4373 1132 Size: 2296 945 3774 Fragments arranged by size: 3774 2296 945 SspI AAT'ATT Cuts at: 5 52 4939 5 Size: 47 4887 2081 Fragments arranged by size: 4887 2081 47 StuI AGG'CCT Cuts at: 1517 3012 1517 Size: 1495 5520 StyI C'CwwG_G Cuts at: 513 1065 2349 3255 3762 4454 513 Size: 552 1284 906 507 692 3074 Fragments arranged by size: 3074 1284 906 692 552 507 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5042 5059 5212 5397 5842 325 Size: 1255 3462 17 153 185 445 1498 Fragments arranged by size: 3462 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3501 3659 4148 Size: 274 80 33 51 1443 1524 158 489 Cuts at: 4148 4260 5261 6973 96 Size: 112 1001 1712 138 Fragments arranged by size: 1712 1524 1443 1001 489 274 158 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2265 2297 3449 3481 6975 7007 2265 Size: 32 1152 32 3494 32 2273 Fragments arranged by size: 3494 2273 1152 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7015 XbaI T'CTAG_A Cuts at: 4351 4351 Size: 7015 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3068 3676 3068 Size: 608 6407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7015 XmaI C'CCGG_G Cuts at: 1870 2808 4340 1870 Size: 938 1532 4545 Fragments arranged by size: 4545 1532 938 XmnI GAAnn'nnTTC Cuts at: 5144 5144 Size: 7015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3717 4823 276 Size: 53 83 186 936 2183 1106 2468 Fragments arranged by size: 2468 2183 1106 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI