(Linear) (Six-base) MAPSORT of: KIBB6266.seq Check: 1939 from: 1 to: 2310 KIBB6266.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2093 2310 Size: 2093 217 AatII G_ACGT'C Cuts at: 0 1714 2310 Size: 1714 596 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 2310 Size: 433 1877 AflIII A'CryG_T Cuts at: 0 1187 1491 1493 2310 Size: 1187 304 2 817 Fragments arranged by size: 1187 817 304 2 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 53 575 2310 Size: 53 522 1735 Fragments arranged by size: 1735 522 53 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 85 117 404 436 2310 Size: 85 32 287 32 1874 Fragments arranged by size: 1874 287 85 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 105 1165 1925 2310 Size: 105 1060 760 385 Fragments arranged by size: 1060 760 385 105 ApaLI G'TGCA_C Cuts at: 0 266 2310 Size: 266 2044 ApoI r'AATT_y Cuts at: 0 94 655 2113 2310 Size: 94 561 1458 197 Fragments arranged by size: 1458 561 197 94 AvaI C'yCGr_G Cuts at: 0 472 803 2271 2310 Size: 472 331 1468 39 Fragments arranged by size: 1468 472 331 39 BanI G'GyrC_C Cuts at: 0 17 988 1882 2310 Size: 17 971 894 428 Fragments arranged by size: 971 894 428 17 BanII G_rGCy'C Cuts at: 0 1558 2310 Size: 1558 752 BglI GCCn_nnn'nGGC Cuts at: 0 877 1219 1231 1544 2310 Size: 877 342 12 313 766 Fragments arranged by size: 877 766 342 313 12 BglII A'GATC_T Cuts at: 0 1976 2310 Size: 1976 334 BlpI GC'TnA_GC Cuts at: 0 389 2227 2310 Size: 389 1838 83 Fragments arranged by size: 1838 389 83 Bme1580I G_kGCm'C Cuts at: 0 270 592 991 1094 2310 Size: 270 322 399 103 1216 Fragments arranged by size: 1216 399 322 270 103 BmrI ACTGGGnnnn_n' Cuts at: 0 412 2310 Size: 412 1898 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 684 986 1016 2310 Size: 684 302 30 1294 Fragments arranged by size: 1294 684 302 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1092 2310 Size: 1092 1218 BsaI GGTCTCn'nnnn_ Cuts at: 0 1024 2310 Size: 1024 1286 BsaAI yAC'GTr Cuts at: 0 1494 2055 2310 Size: 1494 561 255 Fragments arranged by size: 1494 561 255 BsaHI Gr'CG_yC Cuts at: 0 25 1391 1711 2310 Size: 25 1366 320 599 Fragments arranged by size: 1366 599 320 25 BsaWI w'CCGG_w Cuts at: 0 80 2310 Size: 80 2230 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1228 1258 1281 1311 1444 1474 1696 1726 Size: 1228 30 23 30 133 30 222 30 Cuts at: 1726 1954 1984 2310 Size: 228 30 326 Fragments arranged by size: 1228 326 228 222 133 30 30 30 30 30 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 864 2152 2310 Size: 864 1288 158 Fragments arranged by size: 1288 864 158 BseYI C'CCAG_C Cuts at: 0 1565 2310 Size: 1565 745 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1611 1792 2035 2065 2310 Size: 1611 181 243 30 245 Fragments arranged by size: 1611 245 243 181 30 BsiEI CG_ry'CG Cuts at: 0 1223 2310 Size: 1223 1087 BsiHKAI G_wGCw'C Cuts at: 0 270 510 569 2310 Size: 270 240 59 1741 Fragments arranged by size: 1741 270 240 59 BsmI GAATG_Cn' Cuts at: 0 363 2310 Size: 363 1947 Bsp1286I G_dGCh'C Cuts at: 0 270 510 569 592 991 1094 1558 2310 Size: 270 240 59 23 399 103 464 752 Fragments arranged by size: 752 464 399 270 240 103 59 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1338 1940 2093 2310 Size: 1338 602 153 217 Fragments arranged by size: 1338 602 217 153 BsrDI GCAATG_nn' Cuts at: 0 2043 2310 Size: 2043 267 BsrFI r'CCGG_y Cuts at: 0 14 484 2310 Size: 14 470 1826 Fragments arranged by size: 1826 470 14 BsrGI T'GTAC_A Cuts at: 0 1496 2310 Size: 1496 814 BssSI C'ACGA_G Cuts at: 0 1485 2310 Size: 1485 825 BstAPI GCAn_nnn'nTGC Cuts at: 0 381 790 2310 Size: 381 409 1520 Fragments arranged by size: 1520 409 381 BstEII G'GTnAC_C Cuts at: 0 339 2310 Size: 339 1971 BstYI r'GATC_y Cuts at: 0 1803 1976 2310 Size: 1803 173 334 Fragments arranged by size: 1803 334 173 BtgI C'CryG_G Cuts at: 0 344 408 866 1217 1226 1250 1446 2310 Size: 344 64 458 351 9 24 196 864 Fragments arranged by size: 864 458 351 344 196 64 24 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 746 1776 2310 Size: 746 1030 534 Fragments arranged by size: 1030 746 534 BtsI GCAGTG_nn' Cuts at: 0 486 1094 2024 2310 Size: 486 608 930 286 Fragments arranged by size: 930 608 486 286 DraIII CAC_nnn'GTG Cuts at: 0 183 2310 Size: 183 2127 DrdI GACnn_nn'nnGTC Cuts at: 0 1915 2310 Size: 1915 395 EaeI y'GGCC_r Cuts at: 0 405 1220 1739 2310 Size: 405 815 519 571 Fragments arranged by size: 815 571 519 405 EagI C'GGCC_G Cuts at: 0 1220 2310 Size: 1220 1090 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1717 2190 2310 Size: 1717 473 120 Fragments arranged by size: 1717 473 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 684 986 1016 2310 Size: 433 251 302 30 1294 Fragments arranged by size: 1294 433 302 251 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1936 2310 Size: 1936 374 EcoO109I rG'GnC_Cy Cuts at: 0 765 1671 1922 1929 2310 Size: 765 906 251 7 381 Fragments arranged by size: 906 765 381 251 7 EcoRI G'AATT_C Cuts at: 0 94 2310 Size: 94 2216 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 905 1782 2310 Size: 905 877 528 Fragments arranged by size: 905 877 528 HaeII r_GCGC'y Cuts at: 0 998 1166 1208 1245 2310 Size: 998 168 42 37 1065 Fragments arranged by size: 1065 998 168 42 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 682 714 1394 1426 1696 1728 1954 1986 Size: 682 32 680 32 270 32 226 32 Cuts at: 1986 2203 2235 2310 Size: 217 32 75 Fragments arranged by size: 682 680 270 226 217 75 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1053 2310 Size: 1053 1257 HpaI GTT'AAC Cuts at: 0 1053 2310 Size: 1053 1257 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1708 2310 Size: 1708 602 MscI TGG'CCA Cuts at: 0 407 1741 2310 Size: 407 1334 569 Fragments arranged by size: 1334 569 407 MslI CAynn'nnrTG Cuts at: 0 220 557 1795 2087 2242 2310 Size: 220 337 1238 292 155 68 Fragments arranged by size: 1238 337 292 220 155 68 MspA1I CmG'CkG Cuts at: 0 1066 1219 1859 1950 2079 2310 Size: 1066 153 640 91 129 231 Fragments arranged by size: 1066 640 231 153 129 91 NaeI GCC'GGC Cuts at: 0 16 486 2310 Size: 16 470 1824 Fragments arranged by size: 1824 470 16 NcoI C'CATG_G Cuts at: 0 344 1250 2310 Size: 344 906 1060 Fragments arranged by size: 1060 906 344 NdeI CA'TA_TG Cuts at: 0 204 2310 Size: 204 2106 NgoMIV G'CCGG_C Cuts at: 0 14 484 2310 Size: 14 470 1826 Fragments arranged by size: 1826 470 14 NotI GC'GGCC_GC Cuts at: 0 1220 2310 Size: 1220 1090 NspI r_CATG'y Cuts at: 0 978 1191 1423 2310 Size: 978 213 232 887 Fragments arranged by size: 978 887 232 213 PasI CC'CwG_GG Cuts at: 0 1318 1986 2310 Size: 1318 668 324 Fragments arranged by size: 1318 668 324 PciI A'CATG_T Cuts at: 0 1187 2310 Size: 1187 1123 PflMI CCAn_nnn'nTGG Cuts at: 0 1892 2310 Size: 1892 418 PfoI T'CCnGG_A Cuts at: 0 65 456 2310 Size: 65 391 1854 Fragments arranged by size: 1854 391 65 PmlI CAC'GTG Cuts at: 0 1494 2055 2310 Size: 1494 561 255 Fragments arranged by size: 1494 561 255 PpuMI rG'GwC_Cy Cuts at: 0 765 1671 1922 1929 2310 Size: 765 906 251 7 381 Fragments arranged by size: 906 765 381 251 7 PstI C_TGCA'G Cuts at: 0 42 110 1356 1780 1955 2310 Size: 42 68 1246 424 175 355 Fragments arranged by size: 1246 424 355 175 68 42 PvuII CAG'CTG Cuts at: 0 1066 1859 1950 2079 2310 Size: 1066 793 91 129 231 Fragments arranged by size: 1066 793 231 129 91 SacII CC_GC'GG Cuts at: 0 1220 2310 Size: 1220 1090 SbfI CC_TGCA'GG Cuts at: 0 110 2310 Size: 110 2200 ScaI AGT'ACT Cuts at: 0 1656 2145 2310 Size: 1656 489 165 Fragments arranged by size: 1656 489 165 SfcI C'TryA_G Cuts at: 0 38 106 1352 1776 1951 2288 2310 Size: 38 68 1246 424 175 337 22 Fragments arranged by size: 1246 424 337 175 68 38 22 SmaI CCC'GGG Cuts at: 0 805 2310 Size: 805 1505 SmlI C'TyrA_G Cuts at: 0 1071 2310 Size: 1071 1239 SpeI A'CTAG_T Cuts at: 0 1821 2310 Size: 1821 489 SphI G_CATG'C Cuts at: 0 1423 2310 Size: 1423 887 StuI AGG'CCT Cuts at: 0 1007 2310 Size: 1007 1303 StyI C'CwwG_G Cuts at: 0 344 1250 1757 2310 Size: 344 906 507 553 Fragments arranged by size: 906 553 507 344 TatI w'GTAC_w Cuts at: 0 1496 1654 2143 2255 2310 Size: 1496 158 489 112 55 Fragments arranged by size: 1496 489 158 112 55 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 260 292 1444 1476 2310 Size: 260 32 1152 32 834 Fragments arranged by size: 1152 834 260 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1063 1671 2310 Size: 1063 608 639 Fragments arranged by size: 1063 639 608 XmaI C'CCGG_G Cuts at: 0 803 2310 Size: 803 1507 ZraI GAC'GTC Cuts at: 0 1712 2310 Size: 1712 598 Enzymes that do cut: AarI AatII AcuI AflIII AlfI AloI AlwNI ApaLI ApoI AvaI BanI BanII BglI BglII BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NotI NspI PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII SacII SbfI ScaI SfcI SmaI SmlI SpeI SphI StuI StyI TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI Bpu10I BsaBI BsiWI BsmBI BspEI BspHI BsrBI BssHII BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI EarI EcoICRI EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI MluI NarI NheI NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmnI