################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 350 GTCGAC GT^[AC][GT]AC Aor51HI 65 AGCGCT AGC^GCT ApaI 742 GGGCCC GGGCC^C BamHI 243 GGATCC G^GATCC BbeI 328 GGCGCC GGCGC^C BbrPI 208 CACGTG CAC^GTG BclI 257 TGATCA T^GATCA BlpI 68 GCTCAGC GC^T[ACGT]AGC Bpu1102I 68 GCTCAGC GC^T[ACGT]AGC BsaAI 208 CACGTG [CT]AC^GT[GA] BsmFI 165 GTCCC GGGAC(10/14) Bsp1407I 764 TGTACA T^GTACA BspMI 458 ACCTGC ACCTGC(4/8) BsrBI 88 GAGCGG CCGCTC(-3/-3) BsrDI 494 GCAATG GCAATG(2/0) BsrGI 764 TGTACA T^GTACA BssSI 418 CACGAG CACGAG(-5/-1) BstXI 203 CCAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 243 GGATCC [GA]^GATC[CT] CelII 68 GCTCAGC GC^T[ACGT]AGC Eco47III 65 AGCGCT AGC^GCT EcoT14I 248 CCAAGG C^C[AT][AT]GG FbaI 257 TGATCA T^GATCA HgaI 395 GCGTC GACGC(5/10) HincII 350 GTCGAC GT[CT]^[GA]AC HindII 350 GTCGAC GT[CT]^[GA]AC KasI 328 GGCGCC G^GCGCC MflI 243 GGATCC [GA]^GATC[CT] NarI 328 GGCGCC GG^CGCC PmaCI 208 CACGTG CAC^GTG PmlI 208 CACGTG CAC^GTG PpuMI 163 GGGTCCC [GA]G^G[AT]CC[CT] SacI 727 GAGCTC GAGCT^C SalI 350 GTCGAC G^TCGAC SfoI 328 GGCGCC GGC^GCC SmlI 289 CTCAAG C^T[CT][GA]AG SspBI 764 TGTACA T^GTACA StyI 248 CCAAGG C^C[AT][AT]GG TfiI 123 GATTC G^A[AT]TC XhoII 243 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 328 GGCGCC G[GA]^CG[CT]C 394 GGCGTC AspHI 727 GAGCTC G[AT]GC[AT]^C 864 GAGCAC AvaII 164 GGTCC G^G[AT]CC 469 GGTCC BbsI 705 GAAGAC GAAGAC(2/6) 856 GTCTTC BpuAI 705 GAAGAC GAAGAC(2/6) 856 GTCTTC BsaHI 328 GGCGCC G[GA]^CG[CT]C 394 GGCGTC BsaI 272 GAGACC GGTCTC(1/5) 594 GGTCTC BseRI 573 CTCCTC GAGGAG(10/8) 55 GAGGAG BsiHKAI 727 GAGCTC G[AT]GC[AT]^C 864 GAGCAC BsmAI 272 GAGAC GTCTC(1/5) 595 GTCTC BsrI 369 ACTGG ACTGG(1/-1) 278 CCAGT BssHII 99 GCGCGC G^CGCGC 101 GCGCGC DraII 163 GGGTCCC [GA]G^G[ACGT]CC[CT] 742 GGGCCCC DraIII 486 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 691 CACCATGTG EcoO109I 163 GGGTCCC [GA]G^G[ACGT]CC[CT] 742 GGGCCCC Hin1I 328 GGCGCC G[GA]^CG[CT]C 394 GGCGTC MslI 208 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 432 CACACCTGTG PleI 236 GAGTC GAGTC(4/5) 854 GAGTC SfaNI 181 GATGC GCATC(5/9) 423 GCATC SmaI 50 CCCGGG CCC^GGG 324 CCCGGG TaqI 239 TCGA T^CGA 351 TCGA Tsp45I 199 GTGAC ^GT[GC]AC 397 GTCAC TthHB8I 239 TCGA T^CGA 351 TCGA XcmI 456 CCACCTGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 800 CCACAGCAGACGTGG XmaCI 50 CCCGGG C^CCGGG 324 CCCGGG XmaI 50 CCCGGG C^CCGGG 324 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI