(Circular) (Six-base) MAPSORT of: pFN21AE3946.seq Check: 4792 from: 1 to: 5578 pFN21AE3946 5578 bp With 182 enzymes: SgfI * March 28, 2012 11:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2472 2472 Size: 5578 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3388 278 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2356 2921 1937 Size: 419 565 4594 Fragments arranged by size: 4594 565 419 Acc65I G'GTAC_C Cuts at: 2899 2899 Size: 5578 AclI AA'CG_TT Cuts at: 1563 3705 4078 1563 Size: 2142 373 3063 Fragments arranged by size: 3063 2142 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 2783 3641 5025 5293 1632 Size: 704 447 858 1384 268 1917 Fragments arranged by size: 1917 1384 858 704 447 268 AfeI AGC'GCT Cuts at: 2072 2072 Size: 5578 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5381 AflIII A'CryG_T Cuts at: 1203 4477 5454 1203 Size: 3274 977 1327 Fragments arranged by size: 3274 1327 977 AgeI A'CCGG_T Cuts at: 4409 4409 Size: 5578 AhdI GACnn_n'nnGTC Cuts at: 4307 4307 Size: 5578 AleI CACnn'nnGTG Cuts at: 1166 2441 1166 Size: 1275 4303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5578 AlwNI CAG_nnn'CTG Cuts at: 3321 4893 3321 Size: 1572 4006 ApaI G_GGCC'C Cuts at: 2751 2751 Size: 5578 ApaLI G'TGCA_C Cuts at: 3635 4791 3635 Size: 1156 4422 ApoI r'AATT_y Cuts at: 1493 2887 3113 3265 5374 1493 Size: 1394 226 152 2109 1697 Fragments arranged by size: 2109 1697 1394 226 152 AseI AT'TA_AT Cuts at: 160 1032 4132 160 Size: 872 3100 1606 Fragments arranged by size: 3100 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2274 2329 2903 1870 Size: 73 112 219 55 574 4545 Fragments arranged by size: 4545 574 219 112 73 55 BamHI G'GATC_C Cuts at: 2248 2908 3275 2248 Size: 660 367 4551 Fragments arranged by size: 4551 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2534 2694 2829 2899 Size: 325 217 627 546 201 160 135 70 Cuts at: 2899 4354 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 546 325 217 201 160 135 70 BanII G_rGCy'C Cuts at: 1391 2451 2736 2751 2897 1391 Size: 1060 285 15 146 4072 Fragments arranged by size: 4072 1060 285 146 15 BbeI G_GCGC'C Cuts at: 2337 2337 Size: 5578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2717 2854 928 Size: 971 818 137 3652 Fragments arranged by size: 3652 971 818 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3769 3803 1903 Size: 34 50 34 1748 34 3678 Fragments arranged by size: 3678 1748 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3470 4680 3470 Size: 1210 4368 BclI T'GATC_A Cuts at: 1548 2262 1548 Size: 714 4864 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4189 136 Size: 107 122 71 631 605 2517 1525 Fragments arranged by size: 2517 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5573 5573 Size: 5578 BlpI GC'TnA_GC Cuts at: 2074 2995 2074 Size: 921 4657 Bme1580I G_kGCm'C Cuts at: 1790 2374 2438 2537 2634 2751 2789 3639 4795 Size: 584 64 99 97 117 38 850 1156 Cuts at: 4795 1790 Size: 2573 Fragments arranged by size: 2573 1156 850 584 117 99 97 64 38 BmgBI CAC'GTC Cuts at: 1318 2815 1318 Size: 1497 4081 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2277 3309 4267 Size: 416 53 83 399 24 860 1032 958 Cuts at: 4267 442 Size: 1753 Fragments arranged by size: 1753 1032 958 860 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5578 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2211 2813 4238 1239 Size: 956 16 602 1425 2579 Fragments arranged by size: 2579 1425 956 602 16 Bpu10I CC'TnA_GC Cuts at: 2867 2867 Size: 5578 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 3703 4568 4866 2279 Size: 1424 865 298 2991 Fragments arranged by size: 2991 1424 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2271 2605 4241 882 Size: 617 772 334 1636 2219 Fragments arranged by size: 2219 1636 772 617 334 BsaAI yAC'GTr Cuts at: 493 1204 2215 3369 493 Size: 711 1011 1154 2702 Fragments arranged by size: 2702 1154 1011 711 BsaBI GATnn'nnATC Cuts at: 5572 5572 Size: 5578 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 2400 3385 3767 Size: 53 83 186 936 801 66 985 382 Cuts at: 3767 4402 275 Size: 635 1451 Fragments arranged by size: 1451 985 936 801 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 4011 4409 4683 4830 5241 4011 Size: 398 274 147 411 4348 Fragments arranged by size: 4348 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 1178 Size: 30 165 30 1158 30 4165 Fragments arranged by size: 4165 1158 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2569 1186 Size: 889 494 4195 Fragments arranged by size: 4195 889 494 BseYI C'CCAG_C Cuts at: 2337 4781 5277 2337 Size: 2444 496 2638 Fragments arranged by size: 2638 2444 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5005 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3789 4817 1540 Size: 275 186 1027 761 1028 2301 Fragments arranged by size: 2301 1028 1027 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2736 2873 3639 3724 4795 2736 Size: 137 766 85 1071 3519 Fragments arranged by size: 3519 1071 766 137 85 BsmI GAATG_Cn' Cuts at: 3089 3182 3089 Size: 93 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 2438 2451 2537 2634 2736 2751 Size: 399 584 64 13 86 97 102 15 Cuts at: 2751 2789 2873 2897 3639 3724 4795 1391 Size: 38 84 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 584 399 102 97 86 85 84 64 38 24 15 13 BspEI T'CCGG_A Cuts at: 5241 5241 Size: 5578 BspHI T'CATG_A Cuts at: 3467 5197 3467 Size: 1730 3848 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2472 2933 844 Size: 1628 461 3489 Fragments arranged by size: 3489 1628 461 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3465 1394 Size: 429 272 1370 3507 Fragments arranged by size: 3507 1370 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 4073 4247 65 Size: 1183 1258 1567 174 1396 Fragments arranged by size: 1567 1396 1258 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4222 4409 1700 Size: 110 2412 187 2869 Fragments arranged by size: 2869 2412 187 110 BsrGI T'GTAC_A Cuts at: 96 2769 96 Size: 2673 2905 BssHII G'CGCG_C Cuts at: 1925 2104 2106 1925 Size: 179 2 5397 Fragments arranged by size: 5397 179 2 BssSI C'ACGA_G Cuts at: 2423 3638 4650 2423 Size: 1215 1012 3351 Fragments arranged by size: 3351 1215 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5578 BstBI TT'CG_AA Cuts at: 4382 4382 Size: 5578 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5578 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2215 Size: 5578 BstYI r'GATC_y Cuts at: 1972 2248 2908 3275 3660 3677 5129 5215 5227 Size: 276 660 367 385 17 1452 86 12 Cuts at: 5227 5573 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 660 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5026 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4839 Fragments arranged by size: 4839 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3888 3908 3094 Size: 794 20 4764 Fragments arranged by size: 4764 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5578 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5543 DraI TTT'AAA Cuts at: 2882 2882 Size: 5578 DraIII CAC_nnn'GTG Cuts at: 2496 2701 2496 Size: 205 5373 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4585 809 Size: 507 3269 1802 Fragments arranged by size: 3269 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3025 3914 5543 Size: 54 1425 50 275 18 1195 889 1629 Cuts at: 5543 8 Size: 43 Fragments arranged by size: 1629 1425 1195 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 1537 Size: 275 1213 4090 Fragments arranged by size: 4090 1213 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3508 1353 Size: 2155 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4165 4539 4685 387 Size: 1064 138 2576 374 146 1280 Fragments arranged by size: 2576 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2734 2734 Size: 5578 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2211 2336 2783 2813 3641 4238 Size: 393 563 16 125 447 30 828 597 Cuts at: 4238 5025 5293 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 597 563 447 393 268 125 30 16 EcoO109I rG'GnC_Cy Cuts at: 2169 2748 2169 Size: 579 4999 EcoRI G'AATT_C Cuts at: 2887 3265 2887 Size: 378 5200 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2878 2878 Size: 5578 FspI TGC'GCA Cuts at: 4084 4084 Size: 5578 HaeII r_GCGC'y Cuts at: 1678 2074 2337 2827 4725 1678 Size: 396 263 490 1898 2531 Fragments arranged by size: 2531 1898 490 396 263 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 4219 4251 4293 Size: 32 135 32 1097 32 1518 32 42 Cuts at: 4293 4325 1373 Size: 32 2626 Fragments arranged by size: 2626 1518 1097 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2357 2922 3168 669 Size: 988 281 419 565 246 3079 Fragments arranged by size: 3079 988 565 419 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5578 HpaI GTT'AAC Cuts at: 3168 3168 Size: 5578 KasI G'GCGC_C Cuts at: 2333 2333 Size: 5578 KpnI G_GTAC'C Cuts at: 2903 2903 Size: 5578 MfeI C'AATT_G Cuts at: 3177 3177 Size: 5578 MluI A'CGCG_T Cuts at: 5454 5454 Size: 5578 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2523 4692 4876 1305 Size: 1218 2169 184 2007 Fragments arranged by size: 2169 2007 1218 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3756 Fragments arranged by size: 3756 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 2441 3536 3895 4054 518 Size: 648 1051 224 1095 359 159 2042 Fragments arranged by size: 2042 1095 1051 648 359 224 159 MspA1I CmG'CkG Cuts at: 1606 2994 3671 4819 5064 1606 Size: 1388 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 245 NarI GG'CG_CC Cuts at: 2334 2334 Size: 5578 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5026 NdeI CA'TA_TG Cuts at: 387 387 Size: 5578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5578 NotI GC'GGCC_GC Cuts at: 3025 3025 Size: 5578 NspI r_CATG'y Cuts at: 1132 1229 2936 4481 1132 Size: 97 1707 1545 2229 Fragments arranged by size: 2229 1707 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5578 PciI A'CATG_T Cuts at: 4477 4477 Size: 5578 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5578 PmeI GTTT'AAAC Cuts at: 2882 2882 Size: 5578 PmlI CAC'GTG Cuts at: 2215 2215 Size: 5578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3647 3679 5160 5192 3647 Size: 32 1481 32 4033 Fragments arranged by size: 4033 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2169 2169 Size: 5578 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5578 PsiI TTA'TAA Cuts at: 3148 3148 Size: 5578 PspOMI G'GGCC_C Cuts at: 2747 2747 Size: 5578 PstI C_TGCA'G Cuts at: 830 2930 830 Size: 2100 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5578 SacI G_AGCT'C Cuts at: 2736 2736 Size: 5578 SalI G'TCGA_C Cuts at: 1936 2355 2920 1936 Size: 419 565 4594 Fragments arranged by size: 4594 565 419 SanDI GG'GwC_CC Cuts at: 2169 2169 Size: 5578 SbfI CC_TGCA'GG Cuts at: 2930 2930 Size: 5578 ScaI AGT'ACT Cuts at: 3826 5538 3826 Size: 1712 3866 SfcI C'TryA_G Cuts at: 826 1045 2926 4061 4742 4933 826 Size: 219 1881 1135 681 191 1471 Fragments arranged by size: 1881 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2335 2335 Size: 5578 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5578 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5578 SmaI CCC'GGG Cuts at: 1872 2057 2331 2905 1872 Size: 185 274 574 4545 Fragments arranged by size: 4545 574 274 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 3682 4583 4845 820 Size: 197 926 351 1388 901 262 1553 Fragments arranged by size: 1553 1388 926 901 351 262 197 SnaBI TAC'GTA Cuts at: 493 3369 493 Size: 2876 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5578 SphI G_CATG'C Cuts at: 1132 2936 1132 Size: 1804 3774 SspI AAT'ATT Cuts at: 5 52 3502 5 Size: 47 3450 2081 Fragments arranged by size: 3450 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5578 StyI C'CwwG_G Cuts at: 513 1065 2253 3017 513 Size: 552 1188 764 3074 Fragments arranged by size: 3074 1188 764 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3605 3622 3775 3960 4405 325 Size: 1255 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 3824 5536 Size: 274 80 33 51 1443 792 1055 1712 Cuts at: 5536 96 Size: 138 Fragments arranged by size: 1712 1443 1055 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2561 2593 5538 5570 2561 Size: 32 2945 32 2569 Fragments arranged by size: 2945 2569 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5578 XbaI T'CTAG_A Cuts at: 2914 2914 Size: 5578 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2468 2812 2468 Size: 344 5234 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5578 XmaI C'CCGG_G Cuts at: 1870 2055 2329 2903 1870 Size: 185 274 574 4545 Fragments arranged by size: 4545 574 274 185 XmnI GAAnn'nnTTC Cuts at: 3707 3707 Size: 5578 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3386 276 Size: 53 83 186 936 1852 2468 Fragments arranged by size: 2468 1852 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI