(Circular) (Six-base) MAPSORT of: pFN21AB6683.seq Check: 866 from: 1 to: 5986 pFN21AB6683 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3065 3065 Size: 5986 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3796 278 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3329 1937 Size: 1392 4594 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3290 4049 5433 5701 1632 Size: 1658 759 1384 268 1917 Fragments arranged by size: 1917 1658 1384 759 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 4885 5862 1203 Size: 3682 977 1327 Fragments arranged by size: 3682 1327 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 3729 5301 3729 Size: 1572 4414 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2113 2511 2758 2861 3295 3521 3673 5782 Size: 620 398 247 103 434 226 152 2109 Cuts at: 5782 1493 Size: 1697 Fragments arranged by size: 2109 1697 620 434 398 247 226 152 103 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2168 3311 1870 Size: 73 225 1143 4545 Fragments arranged by size: 4545 1143 225 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 3307 4762 618 Size: 325 217 627 291 1229 1455 1842 Fragments arranged by size: 1842 1455 1229 627 325 291 217 BanII G_rGCy'C Cuts at: 1391 3305 1391 Size: 1914 4072 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 5986 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4177 4211 1903 Size: 34 2240 34 3678 Fragments arranged by size: 3678 2240 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BfrBI ATG'CAT Cuts at: 2355 2406 2355 Size: 51 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 4597 136 Size: 107 122 71 631 605 348 2577 1525 Fragments arranged by size: 2577 1525 631 605 348 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 3403 3403 Size: 5986 Bme1580I G_kGCm'C Cuts at: 1790 4047 5203 1790 Size: 2257 1156 2573 Fragments arranged by size: 2573 2257 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5986 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 3717 4675 Size: 416 53 83 399 24 882 1418 958 Cuts at: 4675 442 Size: 1753 Fragments arranged by size: 1753 1418 958 882 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3004 4646 1239 Size: 1765 1642 2579 Fragments arranged by size: 2579 1765 1642 Bpu10I CC'TnA_GC Cuts at: 2420 2420 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3098 4111 4976 5274 3098 Size: 1013 865 298 3810 Fragments arranged by size: 3810 1013 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3168 4649 882 Size: 617 1669 1481 2219 Fragments arranged by size: 2219 1669 1481 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3793 4175 4810 Size: 53 83 186 936 546 1714 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1714 1451 936 635 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2242 4419 4817 5091 5238 5649 2242 Size: 2177 398 274 147 411 2579 Fragments arranged by size: 2579 2177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5986 BseYI C'CCAG_C Cuts at: 5189 5685 5189 Size: 496 5490 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2125 3179 1163 Size: 573 389 1054 3970 Fragments arranged by size: 3970 1054 573 389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4047 4132 5203 4047 Size: 85 1071 4830 Fragments arranged by size: 4830 1071 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3305 4047 4132 5203 1391 Size: 399 1515 742 85 1071 2174 Fragments arranged by size: 2174 1515 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2242 5649 2242 Size: 3407 2579 BspHI T'CATG_A Cuts at: 3875 5605 3875 Size: 1730 4256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3065 3341 844 Size: 2221 276 3489 Fragments arranged by size: 3489 2221 276 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2747 4481 4655 65 Size: 1183 1499 1734 174 1396 Fragments arranged by size: 1734 1499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2054 4630 4817 1700 Size: 110 204 40 2576 187 2869 Fragments arranged by size: 2869 2576 204 187 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5986 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2552 Size: 5986 BstYI r'GATC_y Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981 Size: 1344 367 385 17 1452 86 12 346 Cuts at: 5981 1972 Size: 1977 Fragments arranged by size: 1977 1452 1344 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 3502 4296 4316 3502 Size: 794 20 5172 Fragments arranged by size: 5172 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3160 3290 3160 Size: 130 5856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 3433 4322 Size: 54 1425 50 275 18 182 1421 889 Cuts at: 4322 5951 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1421 889 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3916 1353 Size: 2563 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3004 3290 4049 4646 5433 5701 1239 Size: 393 1372 286 759 597 787 268 1524 Fragments arranged by size: 1524 1372 787 759 597 393 286 268 EcoO109I rG'GnC_Cy Cuts at: 2301 2301 Size: 5986 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 2042 2082 5133 1678 Size: 364 40 3051 2531 Fragments arranged by size: 3051 2531 364 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 2272 2304 2366 Size: 32 135 32 574 32 94 32 62 Cuts at: 2366 2398 2870 2902 4627 4659 4701 4733 1373 Size: 32 472 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 574 472 135 94 62 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3330 3576 669 Size: 988 281 1392 246 3079 Fragments arranged by size: 3079 1392 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KasI G'GCGC_C Cuts at: 2078 2078 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 5862 5862 Size: 5986 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5100 5284 1305 Size: 3795 184 2007 Fragments arranged by size: 3795 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4164 Fragments arranged by size: 4164 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2520 2724 3044 3944 4303 4462 518 Size: 648 1354 204 320 900 359 159 2042 Fragments arranged by size: 2042 1354 900 648 359 320 204 159 MspA1I CmG'CkG Cuts at: 1606 3402 4079 5227 5472 1606 Size: 1796 677 1148 245 2120 Fragments arranged by size: 2120 1796 1148 677 245 NaeI GCC'GGC Cuts at: 2016 2056 2016 Size: 40 5946 NarI GG'CG_CC Cuts at: 2079 2079 Size: 5986 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4495 Fragments arranged by size: 4495 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NgoMIV G'CCGG_C Cuts at: 2014 2054 2014 Size: 40 5946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NsiI A_TGCA'T Cuts at: 2357 2408 2357 Size: 51 5935 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5986 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2617 1108 Size: 902 607 4477 Fragments arranged by size: 4477 902 607 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2301 2301 Size: 5986 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PstI C_TGCA'G Cuts at: 830 3338 830 Size: 2508 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3328 1936 Size: 1392 4594 SanDI GG'GwC_CC Cuts at: 2301 2301 Size: 5986 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 3090 4234 5946 3090 Size: 1144 1712 3130 Fragments arranged by size: 3130 1712 1144 SfcI C'TryA_G Cuts at: 826 1045 3249 3334 4469 5150 5341 826 Size: 219 2204 85 1135 681 191 1471 Fragments arranged by size: 2204 1471 1135 681 219 191 85 SfoI GGC'GCC Cuts at: 2080 2080 Size: 5986 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 2054 1700 Size: 354 5632 SmaI CCC'GGG Cuts at: 1872 3313 1872 Size: 1441 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3113 4090 4991 5253 820 Size: 197 926 1170 977 901 262 1553 Fragments arranged by size: 1553 1170 977 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2004 3231 3425 513 Size: 552 939 1227 194 3074 Fragments arranged by size: 3074 1227 939 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4013 4030 4183 4368 4813 325 Size: 1255 2433 17 153 185 445 1498 Fragments arranged by size: 2433 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3088 4232 5944 Size: 274 80 33 51 1443 1111 1144 1712 Cuts at: 5944 96 Size: 138 Fragments arranged by size: 1712 1443 1144 1111 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5986 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5986 XmaI C'CCGG_G Cuts at: 1870 3311 1870 Size: 1441 4545 XmnI GAAnn'nnTTC Cuts at: 2511 4115 2511 Size: 1604 4382 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3794 276 Size: 53 83 186 936 2260 2468 Fragments arranged by size: 2468 2260 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI